1 research outputs found
The use of non- treated starch for butanol production by Clostridium acetobutylicum
Introduction: Greenhouse
effect problems, environmental pollution, global increase in oil demand, and reduced fossil fuel resources have boosted research on
the production of renewable energies such as bioenegries in recent years. Amongst various biofuels, biobutanol has been recently
introduced as a replacement liquid fuel for gasoline and gas oil. Anaerobic
bacteria such as Clostridium acetobutylicum are
able to produce acetone, butanol, and ethanol using
different sugar sources. This bacterium exhibits amylolytic activity and
therefore is able to hydrolyzes starch to glucose and use it directly as carbon source. Materials and methods: In this study, three substrates including glucose,
treated starch and starch were used in different concentrations to produce
butanol by Clostridium acetobutylicum PTCC
1492. Results: For all three carbon sources, 60 g/ l of
substrate was found as the optimum concentration. The results revealed that
this bacterium is
capable of producing butanol using all three carbon sources without any
treatment. Butanol concentrations of 6.45, 5.81, and 4.64 g/ l were obtained
using non-treated starch, treated starch, and glucose as carbon sources,
respectively. Discussion and conclusion: The results suggested the possibility of using
non-hydrolyzed starch as carbon source for butanol production. Also it was shown that non-treated starch produces
more butanol compared to the treated starch