Differentiated Stem Cells Derived from Rabbit Adipose Tissue Exhibited in ‎Vitro Adipogenesis and Osteogenesis

The multipotent characteristic of rabbit adipose-derived stem cells makes them available and ‎convenient sources for isolating mesenchymal stem cells. The aim of this study was to assess ‎the differentiation in rabbit adipose-derived stem cells pre-committed to produce several ‎mesenchymal lineages in response to inductive extracellular cues to multipotent stromal cells. ‎Three grams of adipose tissue was taken from a subcutaneous region of the nape of the neck ‎and was carefully isolated to obtain mesenchymal stem cells for expanded by fourth passage. In ‎the 4th passage, active growth of mesenchymal stem cells was observed. Furthermore, the ‎research demonstrated the inherent ability of rabbit MSCs to induce differentiation in ‎osteogenic and adipogenic lineages. These mesenchymal stem cells were successfully isolated ‎from adipose tissue which differentiated into either osteocytes or adipocyte-like cells after 21 ‎and 14 days of culturing in specific osteogenic and adipogenic media, respectively. The ‎remarkable differentiation potential of rabbit mesenchymal stem cells is indicated by ‎mineralized deposition to the osteocytes and lipid droplets accumulated in the cytoplasm lipid ‎vacuoles in the adipocytes‎

Effects of omental pedicle transposition on regeneration of neurotmesis sciatic nerve in rabbit

The present study was aimed at providing an understanding of the role of omental pedicle transposition in peripheral nerve regeneration by utilizing an established rabbit sciatic nerve regeneration model. Twelve adult New Zealand White rabbits (2-2.3kg) were divided into two groups (n=6) and acclimatized for 3 weeks. Complete blood examination, liver and kidney function tests were carried out during this period. In Group A, an end-to-end of sciatic nerve segment anastomosis was done, while that of Group B, the nerve anastomosis wrapped with omental pedicle was performed. The nerve specimens were collected from both groups for histopathological and ultrastructural evaluation after 16 weeks post surgery. Results showed that omental pedicle transpositioned (Group B) had more newly developed nerve fibres and less scar tissue. Ultrastructural examinations showed neuronal sprouting, whereas directions of regenerative nerve fibres were intraneural, but in the end-to-end anastomosis of group B showed that some of nerve fibres had extraneural

Neurobiological observations of bone mesenchymal stem cells in vitro and in vivo of injured sciatic nerve in rabbit

The PKH26 is a fluorescent lipophilic dyes used for the study of Asymmetric cell Divisions (ASDs) and efficiently purifies the stem cell fraction. The aim of this study was to explore the neurobiological characteristics in vitro and in vivo and tracking fate of the transplanted rabbit Bone Marrow-Mesenchymal Stem Cells (rBM-MSCs). A fluorescent microscope was used to determine the changes in cell size, fluorescence intensity during tissue culture, track cell divisions and the distribution of PKH26 dye between daughter cells. The results showed the identification of ASDs based on fluorescence intensity of the PKH26 dye was distributed equally between daughter cells at each division in vitro. The labeling BMSCs with PKH26 showed within the wall of the neurons in the dorsal root ganglia in vivo. Labeled BMSCs which are fibroblastic-like cells in P4 showed oval shaped and less density than P2. Direct examine of the labeled BMSCs in the cryosections at 16 weeks post operation showed the BMSCs were differentiated and appeared as like Schwann cells in an anastomosed sciatic nerve in the Local Treated Group (LTG). In the Systemic Treated Group (STG) sections, the labeled BMSCs were migrated to the anastomosed sciatic nerve, ipsilateral lumber dorsal root ganglia resembling glial and stellate cells and some of the labeled cells migrated to the anterior horn of spinal cord (motor neuron). In conclusion, the biological behaviors of BMSCs in vitro and in vivo showed highly mitosis at P2, activated fibroblast-like cells, differentiated to functional myelinating Schwann-like cells in LTG. The BMSCs in STG migrated and engrafted at the dorsal root ganglia as a neuron and glial cell, glial cells and satellite in the spinal cord

Identification of adipogenesis and osteogenesis pathways of differentiated bone marrow stem cells in vitro in rabbits

Transdifferentiation is a process whereby a cell type committed to and progressing along a specific developmental lineage switches into another cell types. The objective of this study was to assess whether rabbit mesenchymal stem cells (rMSCs) precommitted to give mesenchymal cell lineage transdifferentiate in response to inductive extracellular cues to expand adult MSCs. Bone mesenchymal stem cells (BMSCs) obtained from ilium of adult male rabbit comprised heterogeneous groups of cells after seeding and growing in culture plates. After initial plating, the adherent cells exhibited small rounded, spindle-shaped and exhibited fibroblast-like morphology in reaching confluence. Rabbit BMSCs differentiated into adipocytes and osteocyte as a accumulation of intracellular lipid droplets and calcium deposition throughout the culture after 21 days

Bone marrow stromal cells implantation and suture repair of peripheral nerve: a comparative study of functional, histopathological, morphometric and relative gastrocnemius muscle weight in rabbits

The peripheral nervous system has the ability to regenerate after injury. Peripheral nerve injuries are caused by penetrating injury, crush, traction and ischemia compression. However, the availability of various nerve coaptation and other techniques for the attainment of functional nerve regeneration is still inadequate. The objective of this study was to compare the effectiveness of bone marrow stromal cells (BMSCs) implantation and epineural nerve suture on peripheral nerve regeneration in a rabbit model. Ten male New Zealand white rabbits were divided into two groups. In the primary epineural repair group (control group), the left sciatic nerve was skeletonized from the sciatic notch to the point of bifurcation, with the nerve been transected at the mid-shaft of the femoral bone and repaired with six epineural sutures. In the treated group, the epineural repaired nerve was implanted with BMSCs in the proximal and distal segments of the transected sciatic nerve. Assessment of the nerve regeneration was based on functional (motor and sensory), histological and morphometric criteria, including the number of myelinated nerve fibers, nerve fiber diameter, axon diameter, myelin sheath thickness, g ratio and relative gastrocnemius muscle weight. The results of the examination showed that the treated group had the best regeneration and functional recovery

Prospective open pilot study on the use of cam oleum ointment for feline calicivirus disease in cats in Baghdad City

Feline calicivirus (FCV) is a highly contagious virus that causes a mild to severe respiratory infection and oral disease in cats. It is especially common in shelters and breeding colonies, and often infects young cats, this study contained 50 different cats and samples were collected from September 2020 to January 2021. Samples taken by swabs from oropharyngeal and conjunctival area depending on the lesion of FCV infection to investigate viral nucleic acid from collected swabs, then extracting RNA from the swabs and converting it to a cDNA molecule, and last detecting the open reading template gene 2 using specific primer, these samples isolated from veterinary clinics, and shelters, all samples were collected from Baghdad city .Rapid test AG kit and Real time PCR were done to confirm the detection and the results gave us 28 positive results on rapid AG kit and RT-PCR results gave us 32 positive samples and the other 18 samples gave us negative results by detection with nanodrop device before sending all samples for molecular investigation by polymerase chain reaction. Most cats recover completely after a calicivirus infection when we treated with Camoleum ointmentprepared in veterinary medicine college lab

Prospective Open Pilot Study on the Use of Cam Oleum Ointment for Feline Calicivirus Disease in Cats in Baghdad City

Feline calicivirus (FCV) is a highly contagious virus that causes a mild to severe respiratory infection and oral disease in cats. It is especially common in shelters and breeding colonies, and often infects young cats, this study contained 50 different cats and samples were collected from September 2020 to January 2021. Samples taken by swabs from oropharyngeal and conjunctival area depending on the lesion of FCV infection to investigate viral nucleic acid from collected swabs, then extracting RNA from the swabs and converting it to a cDNA molecule, and last detecting the open reading template gene 2 using specific primer, these samples isolated from veterinary clinics, and shelters, all samples were collected from Baghdad city .Rapid test AG kit and Real time PCR were done to confirm the detection and the results gave us 28 positive results on rapid AG kit and RT-PCR results gave us 32 positive samples and the other 18 samples gave us negative results by detection with nanodrop device before sending all samples for molecular investigation by polymerase chain reaction. Most cats recover completely after a calicivirus infection when we treated with Camoleum ointmentprepared in veterinary medicine college lab