Screening and optimization of biosurfactant producing bacteria from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are amphiphilic compounds produced on living surfaces, mostly on microbial cell surfaces. They are biodegradable non- toxic and ecofreindly materials. Pseudomonas aeruginosa isolated from oil mill area produced biosurfactant at 370C at pH 7 on YPG medium and mineral salt medium. The biosurfactant production depends on the fermentation conditions, environmental factors and nutrient availability. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure & the qualitative and quantitative analysis has been discussed with appropriates equipment details

Separation and characterization of biosurfactant from P.aeruginosa sp1 isolated from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are an amphiphilic compound that reduces surface and interfacial tensions by accumulating at the interface of immiscible fluids and increase the surface areas of insoluble compounds leading to increased mobility. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure and the quantitative analysis has been discussed. The application of the biosurfactant includes biomedical, cosmetics and bioremediation rhamnolipid biosurfactant produced by Pseudomonas aeruginosa sp showed significant applications in the bioremediation of hydrocarbon in gasoline spilled oil and petroleum oily sludge in this review we discuss the potential roles and applications of biosurfactant, mainly focusing on areas such as food and food related industries, biomedicine and therapeutics

Studies on production of peptide antibiotic by thermotolerant Bacillus sp.

The soil sample was collected and screened for isolation of higher antibiotic producing bacterial strain. The isolated bacterial strain was tentatively identified as Bacillus sp. by biochemical characters using Bergey’s manual. The present study is focused on the optimization of different parameters for production of peptide antibiotic (Bacitracin) using synthetic medium. The antibacterial activity of isolate was analyzed against S. aureus. The nutritional ingredients for bacitracin production were found as glucose (1%) and L-glutamic acid (0.5 %) as carbon and nitrogen source respectively. The prominent bacitracin production was obtained after 48 hrs incubation at 40°C temperature and at 7 pH