Feasibility Study for Investigations of (pol.p, p'γ) Reactions with the K600 Spectrometer

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Measurements of (p,p) and (p,p'γ) Observables for 1+ States in 12-C at 200 MeV

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Simultaneous Measurements of (p,p') and (p,p'γ) Observables for the 15.11 MeV, 1+, T=1 State in 12-C at 200 MeV

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Antigenic Constituents in Pregnancy Plasma which are Undetectable in Normal Non-Pregnant Female or Male Plasma

Up to four antigenic constituents have been found in the plasma of pregnant women which are not detectable in specimens from normal untreated non-pregnant women, or from men. They are visualized by immunodiffusion methods with hyperimmune rabbit antiserum to pregnancy plasma, after exhaustive absorption with lyophilized normal untreated non-pregnant female plasma. These constituents are distinct from each other as judged by their differing electrophoretic mobilites. It is unlikely that they represent any of the known normal plasma proteins visualized by gel diffusion because of the absorption methods used. In addition, it has been shown that none of them represent human chorionic gonadotropin, human placental lactogen, oxytocin, C-reactive protein, oxytocinase (cystine-amino-peptidase), alkaline phosphatase or esterase, and they are unrelated to bovine prolactin. Preliminary data revealed that one of these pregnancy-associated plasma constituents is synthesized during the course of oral hormone contraception.</jats:p

Immunological Comparison of Various Human Pregnancy-Associated Plasma Proteins

Direct immunodiffusion comparicon with specific antisera demonstrated that all of the four pregnancy-associated plasma proteins (PAPPs) described in our laboratory are distinct from the pregnancy zone protein (von Schoultz); (α&lt;sub&gt;2&lt;/sub&gt;-pregnoglobulin (Berne); pregnancy-associated α&lt;sub&gt;2&lt;/sub&gt;-glycoprotein (SP&lt;sub&gt;3&lt;/sub&gt;) (Bohn); new serum α&lt;sub&gt;2&lt;/sub&gt;-macroglobulin (Stimson); PAG (Horne); pregnancy-associated α&lt;sub&gt;2&lt;/sub&gt;-globulin (Kasukawa); Pal (McLaren), and Xh protein (Dunston). All the latter proved to be immunologically identical to each other, and apparently represent the same protein described under different names. It was confirmed that none of the PAPPs is immunochemically related to placental alkaline phosphatase. PAPP-A, PAPP-C, and the pregnancy zone protein, but not HPL (PAPP-D), showed a decreased anodic mobility when treated with neuraminidase; their reactivities with antibody were essentially unaffected by the enzyme, however. Certain detergents had no effect on the immunological reactivities of the PAPPs and the pregnancy zone protein in whole plasma, while butanol and desoxycholate partially, and urea, SDS and cetylpyridinium largely inactivated them.</jats:p

Studies on the Origins of ‘Tissue’ Antigens and Enzymes in Normal Human Urine

Antisera to a variety of nongenitourinary tissues (pancreas, submaxillary gland, liver and heart) revealed numerous constituents in normal human urine. By the methods used, these urinary antigens were not related to plasma proteins, and in the case of antipancreas and antisubmaxillary gland sera, several of them apparently were also &lt;i&gt;not&lt;/i&gt; derived from kidney, or from the remainder of the genitourinary tract. The evidence suggested that some of these urinary antigens may have originated in nongenitourinary tissues, entered the circulation in trace quantities, being preferentially concentrated and excreted by the kidney into the urine. Urine concentrates appeared to be relatively enriched in several of the ‘tissue’ antigens detected with these antisera, as compared to extracts of the tissue used for immunization. Five of the urinary ‘tissue’ antigens were found to possess enzyme activities (esterase, phosphatase, cystine-amino-peptidase, glucosaminidase and glucuronidase). Their organ distribution was variable, ranging from presence in all the tissues tested, to detection only in urine.</jats:p