Measuring the through-plane and in-plane oxygen apparent diffusion coefficients in the gas diffusion layer

Accurately predicting oxygen mass transport resistance and current distribution in fuel cells requires significant knowledge of oxygen diffusion coefficients in both in-plane and through-plane directions. However, there are few methods for measuring in-plane oxygen diffusion coefficients, which are important parameters for simulating oxygen flux distribution in gas diffusion layers (GDLs). In this study, we establish measurement methods and calculations for both in-plane and through-plane oxygen diffusion coefficients. Using in-house designed cells, we measure the in-plane and through-plane oxygen apparent diffusion coefficients of a commercial carbon paper (AvCarb EP40) at various torques and gas flow rates. We also simulate oxygen flux distributions in the GDL under each torque and gas flow rate. Our results show that the channel part is the major contributor to total oxygen flux at high torque conditions and that an increase in torque leads to a decrease in the contribution from the land part. Simulation results also suggest that a higher gas flow rate and lower torque contribute to a more uniform distribution of oxygen flux in the GDL

Multi-wavelength quantitative polarization and phase microscope

We introduce a snapshot multi-wavelength quantitative polarization and phase microscope (MQPPM) for measuring spectral dependent quantitative polarization and phase information. The system uniquely integrates a polarized light microscope and a snap-shot quantitative phase microscope in a single system, utilizing a novel full-Stokes camera operating in the red, green, and blue (RGB) spectrum. The linear retardance and fast axis orientation of a birefringent sample can be measured simultaneously in the visible spectra. Both theoretical analysis and experiments have been performed to demonstrate the capability of the proposed microscope. Data from liquid crystal and different biological samples are presented. We believe that MQPPM will be a useful tool in measuring quantitative polarization and phase information of live cells. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing AgreementNational Science Foundation (NSF) [1455630, 1607358]; National Institutes of Health (NIH) [S10OD018061]; TRIF Space Exploration & Optical Sciences (SEOS) of University of ArizonaOpen access journal.This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Abundant aftershock sequence of the 2015 M_w7.5 Hindu Kush intermediate-depth earthquake

The 2015 M_w7.5 Hindu Kush earthquake occurred at a depth of 213 km beneath the Hindu Kush region of Afghanistan. While many early aftershocks were missing from the global earthquake catalogues, this sequence was recorded continuously by eight broad-band stations within 500 km. Here we use a waveform matching technique to systematically detect earthquakes around the main shock. More than 3000 events are detected within 35 d after the main shock, as compared with 42 listed in the Advanced National Seismic System catalogue (or 196 in the International Seismological Centre catalogue). The aftershock sequence generally follows the Omori's law with a decay constant p = 0.92. We also apply the recently developed double-pair double-difference technique to relocate all detected aftershocks. Most of them are located to the west of the hypocentre of the main shock, consistent with the westward propagation of the main-shock rupture. The aftershocks outline a nearly vertical southward dipping plane, which matches well with one of the nodal planes of the main shock. We conclude that the aftershock sequence of this intermediate-depth earthquake shares many similarities with those for shallow earthquakes and infer that there are some common mechanisms responsible for shallow and intermediate-depth earthquakes

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway

Oxidative stress can induce bone tissue damage and the occurrence of multiple diseases. As a type of traditional medicine, tocopherol has been reported to have a strong antioxidant effect and contributes to osteogenic differentiation. The purpose of this study was to investigate the protective effect of tocopherol on the oxidative stress of rat bone marrow-derived mesenchymal stem cells (BMSCs) and the underlying mechanisms. By establishing an oxidative stress model in vitro, the cell counting kit-8 (CCK-8), reactive oxygen species (ROS) analysis, Western blot (WB), real-time PCR (RT-PCR), alkaline phosphatase (ALP) staining, and Alizarin Red staining (ARS) evaluated the effects of tocopherol on the cell viability, intracellular ROS levels, and osteogenic differentiation in BMSCs. In addition, ferroptosis-related markers were examined via Western blot, RT-PCR, and Mito-FerroGreen. Eventually, the PI3K/AKT/mTOR signaling pathway was explored. We found that tocopherol significantly maintained the cell viability, reduced intracellular ROS levels, upregulated the levels of anti-oxidative genes, promoted the levels of osteogenic-related proteins, and the mRNA of BMSCs stimulated by H2O2. More importantly, tocopherol inhibited ferroptosis and upregulated the phosphorylation levels of PI3K, AKT, and mTOR of BMSCs upon H2O2 stimulation. In summary, tocopherol protected BMSCs from oxidative stress damage via the inhibition of ferroptosis through the PI3K/AKT/mTOR pathway

Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood–Spinal Cord Barrier After Spinal Cord Injury in Mice

Spinal cord injury (SCI) often leads to severe and permanent paralysis and places a heavy burden on individuals, families, and society. Until now, the therapy of SCI is still a big challenge for the researchers. Transplantation of mesenchymal stem cells (MSCs) is a hot spot for the treatment of SCI, but many problems and risks have not been resolved. Some studies have reported that the therapeutic effect of MSCs on SCI is related to the paracrine secretion of cells. The exosomes secreted by MSCs have therapeutic potential for many diseases. There are abundant pericytes which possess the characteristics of stem cells in the neurovascular unit. Due to the close relationship between pericytes and endothelial cells, the exosomes of pericytes can be taken up by endothelial cells more easily. There are fewer studies about the therapeutic potential of the exosomes derived from pericytes on SCI now. In this study, exosomes of pericytes were transplanted into the mice with SCI to study the restoration of motor function and explore the underlying mechanism. We found that the exosomes derived from pericytes could reduce pathological changes, improve the motor function, the blood flow and oxygen deficiency after SCI. In addition, the exosomes could improve the endothelial ability to regulate blood flow, protect the blood-spinal cord barrier, reduce edema, decrease the expression of HIF-1α, Bax, Aquaporin-4, and MMP2, increase the expression of Claudin-5, bcl-2 and inhibit apoptosis. The experiments in vitro proved that exosomes derived from pericytes could protect the barrier of spinal cord microvascular endothelial cells under hypoxia condition, which was related to PTEN/AKT pathway. In summary, our study showed that exosomes of pericytes had therapeutic prospects for SCI