Polymorphism in Growth Hormone Gene and its Association with Growth Traits in Siniperca chuatsi

Growth hormone (GH) is a candidate gene for growth traits in fish. In this study, we assessed associations between single nucleotide polymorphisms (SNPs) in GH gene with growth traits in 357 Siniperca chuatsi individuals using high-resolution melting. Two SNPs were identified in GH gene, with one mutation in exon 5 (g.5045T>C), and one mutation in intron 5 (g.5234T>G). The corrections analysis of SNPs with the four growth traits was carried out using General Linear Model (GLM) estimation. Results showed that both of them were significantly associated with growth performance in S. chuatsi. For g.5234T>G, it was significantly associated with body weight (P＜0.01), body length (P＜0.05), body depth (P＜0.01), and body width (P＜0.01), and the individuals of genotype GG grew faster than those of genotypes TT and TG (P＜0.05). A further diplotype-trait association analysis confirmed that in fish with H3H2 (TC-GG) diplotype body weight, body length, and body width was greater than in those with other diplotypes (P＜0.05). These results demonstrated GH gene SNPs could be used as potential genetic markers in future marker assisted selection of S. chuatsi

Experimental investigation into rock burst proneness of rock materials considering strain rate and size effect

In deep rock engineering, evaluating the likelihood of rock burst is imperative to ensure safety. This study proposes a new metric, the post-peak dissipated energy index, which accounts for strain rate and size effects in assessment of the rock burst proneness of a rock mass. To investigate rock burst proneness, conventional compression tests were conducted on limestone and slate samples with different length to diameter (L/D) ratios (ranging from 0.3 to 1.5) at four different strain rates (0.005, 0.01, 0.5, and 1.0 s−1). Based on the testing observations, the actual rock burst proneness was classified into three categories (no risk, low risk, and high risk). A new criterion was also established using the post-peak dissipated energy index, which is the ratio of elastic energy to total dissipated energy. The impact of the strain rate and L/D ratio on rock burst proneness was analyzed. The results indicated that increased strain rates cause a strong hardening effect, leading to staged growth of rock burst proneness. However, the rock burst proneness decreases non-linearly with the increasing L/D ratio. The accuracy of the proposed criterion was validated by comparison with existing criteria, demonstrating that the energy-based index ensures a reliable evaluation of the rock burst proneness of a rock mass. The proposed method has excellent potential for practical application in deep rock engineering

Estimation of mechanics parameters of rock in consideration of confining pressure using monitoring while drilling data

During the drilling process, high-strength rock can lead to various issues such as drilling suppression, bit wear, and increased operational costs. To ensure safe and efficient drilling operations, it is crucial to accurately predict the strength parameters of the rock and recommend modifications to operational procedures. This paper proposes a low-cost and fast measurement method for predicting the strength parameters of rock in the field. To evaluate the effectiveness of this method, a drilling process monitoring experiment was conducted on sandstone, limestone, and granite. The experiment studied the effect of confining pressure on the response of cutting with an impregnated diamond bit. By analyzing the relationship between the thrust force, torque force, and penetration depth under different confining pressures, the researchers developed an analytical model for drilling that considers confining pressure, compressed crushed zone, and bit geometry. The results show that the confining pressure has a significant effect on the cutting response. As the confining pressure increases, the thrust force, torque force, and penetration depth at the cutting point also increase. Furthermore, a new measurement method was proposed to determine the strength parameters, such as cohesion, internal friction angle, and unconfined compressive strength. The estimated strength parameters for the three rock types using the drilling method were in good agreement with those of the standard laboratory test, with an error range of 10%. This method of estimating rock strength parameters is a practical tool for engineers. It can continuously and quickly obtain the drilling parameters of in-situ rocks

Short-interval second ejaculation improves sperm quality, blastocyst formation in oligoasthenozoospermic males in ICSI cycles: a time-lapse sibling oocytes study

BackgroundDoes short-interval second ejaculation improve sperm quality, embryo development and clinical outcomes for oligoasthenozoospermia males received intracytoplasmic sperm injection (ICSI) treatment?MethodsAll enrolled male patients underwent short-interval secondary ejaculation on the day of oocyte retrieval, and 786 sibling MII oocytes from 67 cycles were equally divided into two groups based on whether the injected spermatozoons originated from the first or second ejaculation. Semen parameters, embryo development efficiency, morphokinetic parameters and clinical outcomes were compared between the two groups to assess the efficiency and clinical value of short-interval second ejaculation in ICSI cycles.ResultsShort-interval second ejaculation significantly improved sperm motility, normal morphological rate, and sperm DNA integrity both before and after sperm swim-up. The high-quality blastocyst rate (24.79% versus 14.67%), available blastocyst rate (57.56% versus 48.44%), and oocyte utilization rate (52.93% versus 45.29%) were significantly higher in the second ejaculation group (P<0.05). The clinical pregnancy rate (59.09% versus 47.37%), implantation rate (42.11% versus 32.35%) and live birth rate (40.91% versus 31.58%) were higher in the second ejaculation group, but the differences were not significant (P>0.05). Time-lapse analysis showed that morphokinetic time points after the 7-cell stage were earlier in the second ejaculation group but without a significant difference (P>0.05), and abnormal embryo cleavage patterns between the two groups were not significantly different (P>0.05).ConclusionsShort-interval second ejaculation significantly improves sperm quality in oligoasthenozoospermic males, and is beneficial for blastocyst formation efficiency in ICSI cycles. This study suggested a non-invasive and simple but effective strategy for improving ICSI treatment outcomes

p150 ADAR1 isoform involved in maintenance of HeLa cell proliferation

BACKGROUND: RNA-specific adenosine deaminase ADAR1 is ubiquitously expressed in a variety of mammalian cells and tissues. Although its physiological importance in non-nervous tissues has been confirmed by analysis of null mutation phenotypes, few endogenous editing substrates have been identified in numerous peripheral tissues and biological function of ADAR1 has not been fully understood. METHODS: A conditional site-specific, ribozyme-based gene knock-down strategy was utilized to study the function of full-length isoform of ADAR1 (p150 protein) in HeLa cell. Double-stable HeLa cell lines were developed by transfecting HeLa Tet-On cells with a pTRE-derived plasmid that can express a hammerhead ribozyme against mRNA of p150 ADAR1 isoform under induction condition. Semi-quantitative RT-PCR and Western blotting were performed to measure the expression of p150 in selected cell clones. Cell proliferation was evaluated by means of MTT assay and growth curve analysis. Cellular morphological changes were observed under light microscope. Flow Cytometry was used for cell cycle analysis. Growth rate of cell transplants in BALB/c nude mice was also investigated. RESULTS: Both HeLa cell proliferation in vitro and the growth rate of transplanted HeLa cell-derived tumors in nude mice in vivo were significantly inhibited due to reduced expression of ADAR1 p150. Additionally, cell cycle analysis showed that cell progression from G1 phase to S phase was retarded in the ADAR1 p150 suppressed cells. CONCLUSION: Our results suggest that normal expression and functioning of p150 ADAR1 is essential for the maintenance of proper cell growth. The mechanisms underlying ADAR1's action might include both editing of currently unknown double-stranded RNAs and interacting with other cellular dsRNA-related processes

ERROR PERFORMANCE OF SINGLE PULSE PPK SYSTEM WITH MAXIMUM VALUE TYPE DECISION MODEL

International Telemetering Conference Proceedings / October 24-27, 1983 / Sheraton-Harbor Island Hotel and Convention Center, San Diego, CaliforniaThe error performance of single pulse PPK system with maximum value type decision model is discussed in this paper. The word error probability in the case when noises exist is analysed and the energy necessary for transmitting each information bit is calculated. The detection characteristics of this system are made clear by comparing it with a threshold type decision model and ordinary systems. It is shown that the maximum value type of decision scheme is always superior to the threshold type. It can reduce the word error probability of PPK system further or make the energy efficiency of PPK system higher under the condition of given tolerable error probability.International Foundation for TelemeteringProceedings from the International Telemetering Conference are made available by the International Foundation for Telemetering and the University of Arizona Libraries. Visit http://www.telemetry.org/index.php/contact-us if you have questions about items in this collection

Gibberellins regulate iron deficiency-response by influencing iron transport and translocation in rice seedlings (oryza sativa)

Background and aims Gibberellins (GAs) are a class of plant hormones with diverse functions. However, there has been little information on the role of GAs in response to plant nutrient deficiency. Methods To evaluate the roles of GAs in regulation of Fe homeostasis, the effects of GA on Fe accumulation and Fe translocation in rice seedlings were investigated using wild-type, a rice mutant (euil)displaying enhnaced endogenous GA concentrations due to a defect in GA deactivation, and transgenic rice plants overexpressing OsEUI. Key Results Exposure to Fe-deficient medium significantly reduced biomass of rice plants. Both exogenous application of GA and an endogenous increase of bioactive GA enhanced Fe-deficiency response by exaggerating foliar chlorosis and reducing growth. Iron deficiency significantly suppressed production of GA(1) and GA(4), the biologically active GAs in rice. Exogenous application of GA significantly decreased leaf Fe concentration regardless of Fe supply. Iron concentration in shoot of eui1 mutants was lower than that of WT plants under both Fe-sufficient and Fe-deficient conditions. Paclobutrazol, an inhibitor of GA biosynthesis, alleviated Fe-deficiency responses, and overexpression of EUI significantly increased Fe concentration in shoots and roots. Furthermore, both exogenous application of GA and endogenous increase in GA resulting from EUI mutation inhibited Fe translocation within shoots by suppressing OsYSL2 expression, which is involved in Fe transport and translocation. Conclusions The novel findings provide compelling evidence to support the involvement of GA in mediation of Fe homeostasis in strategy II rice plants by negatively regulating Fe transport and translocation