Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells

The transduction efficiency of Ad (adenovirus) depends, to some extent, on the expression level of CAR (coxsackievirus and Ad receptor) of a target cell. The low level of CAR on the cell surface is a potential barrier to efficient gene transfer. To overcome this problem, PTD.AdeGFP (where eGFP is enhanced green fluorescent protein) was constructed by modifying the HI loop of Ad5 (Ad type 5) fibre with the Tat (trans-activating) PTD (protein transduction domain) derived from HIV. The present study showed that PTD.AdeGFP significantly improved gene transfer to multiple cell types deficient in expression of CAR. The improvement in gene transfer was not the result of charge-directed binding between the virus and the cell surface. Although PTD.AdeGFP formed aggregates, it infected target cells in a manner different from AdeGFP aggregates precipitated by calcium phosphate. In addition, PTD.AdeGFP was able to transduce target cells in a dynamin-independent pathway. The results provide some new clues as to how PTD.AdeGFP infects target cells. This new vector would be valuable in gene-function analysis and for gene therapy in cancer

Local Density Approximation for the Short-Range Exchange Free Energy Functional

10.1021/acsomega.9b00303ACS Omega447675-768

Shallow Crustal Structure of S-Wave Velocities in the Coastal Area of South China Constrained by Receiver Function Amplitudes

As a traditional method, passive seismic exploration is used to construct the body-wave velocity structure of the upper crust, but it is cost-ineffective and depth-limited when applied to large areas. In this study, we use another more economical method to determine the S-wave velocity (SWV) of the upper crust based on the principle that the amplitude of the direct P-wave on the teleseismic receiver function is sensitive to the upper crust. Using the amplitudes of the massive receiver functions from permanent broadband seismic stations, the SWV structure of the upper crust is obtained in the coastal area of South China (CASC). A pattern of high to low SWVs is exhibited across the study area, with SWVs varying about 2.5–3.7 km/s from west to east. In the profile parallel to the coastline, lateral variations in the SWV correspond to the fault zone, indicating that the cutting depth of most coastal faults is approximately 10 km. Referring to previous studies, we deduce that the low SWV in most sub-areas can be interpreted as the joint effect of the sedimentary layer of the alluvial plain and the accumulation of underground heat flows, in addition to multistage fracturing tectonism. Moreover, the gradual change in the SWV in each profile from the surface to approximately 10 km is correlated with multiple invasions and the coverage of volcanic rocks, to a certain extent

Shallow Crustal Structure of S-Wave Velocities in the Coastal Area of South China Constrained by Receiver Function Amplitudes

As a traditional method, passive seismic exploration is used to construct the body-wave velocity structure of the upper crust, but it is cost-ineffective and depth-limited when applied to large areas. In this study, we use another more economical method to determine the S-wave velocity (SWV) of the upper crust based on the principle that the amplitude of the direct P-wave on the teleseismic receiver function is sensitive to the upper crust. Using the amplitudes of the massive receiver functions from permanent broadband seismic stations, the SWV structure of the upper crust is obtained in the coastal area of South China (CASC). A pattern of high to low SWVs is exhibited across the study area, with SWVs varying about 2.5–3.7 km/s from west to east. In the profile parallel to the coastline, lateral variations in the SWV correspond to the fault zone, indicating that the cutting depth of most coastal faults is approximately 10 km. Referring to previous studies, we deduce that the low SWV in most sub-areas can be interpreted as the joint effect of the sedimentary layer of the alluvial plain and the accumulation of underground heat flows, in addition to multistage fracturing tectonism. Moreover, the gradual change in the SWV in each profile from the surface to approximately 10 km is correlated with multiple invasions and the coverage of volcanic rocks, to a certain extent

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Plant-specific WRKY transcription factors are widely involved in abiotic stress responses. In this study, a WRKY gene was isolated from Malus baccata (L.) Borkh and designated it as MbWRKY2. Subcellular localization showed that MbWRKY2 was localized in the nucleus. The expression levels of MbWRKY2 were up-regulated by abiotic stresses in M. baccata. When MbWRKY2 was introduced into tobacco, it improved drought stress tolerance in transgenic plants. The transgenic tobaccos had the higher contents of chlorophyl, proline, relative water content, AsA, and GSH, increased activities of CAT, APX, SOD, and POD, and decreased levels of MDA, H2O2, and electrolyte leakage than wild-type, especially when dealt with dehydration treatment. Moreover, the MbWRKY2-OE plants enhanced the expression of oxidative stress response and stress-related genes involved in osmotic adjustment and membrane protection. These results suggest that MbWRKY2 gene plays a positive regulatory role in drought stress response

Isolation and characterization of MbWRKY1, a WRKY transcription factor gene from Malus baccata (L.) Borkh involved in drought tolerance

WRKY transcription factors are involved in stress responses in plants. However, their roles in abiotic stresses are still not well known in Malus plants. In the present study, a WRKY gene was isolated from Malus baccata (L.) Borkh and designated as MbWRKY1. Subcellular localization revealed that MbWRKY1 was localized in nucleus. The expression levels of MbWRKY1 were up-regulated by dehydration, salinity, and ABA treatments in M. baccata seedlings. When MbWRKY1 was introduced into tobacco, it improved drought stress tolerance in transgenic plants. When dealt with drought treatment, the transgenic plants had the higher contents of chlorophyll, proline, relative water content, AsA and GSH than WT. Compared to WT plants, the over-expression of MbWRKY1 in transgenic tobacco also led to decreased levels of H2O2, MDA, and elecrolyte leakage when dealt with drought stress. There were increased activities of POD, CAT, SOD and APX in transgenic tobaccos, especially when dealt with drought treatment. Moreover, the MbWRKY1 transgenic plants enhanced the expressions of oxidative stress response (NtPOD, NtCAT, NtSOD and NtAPX) and stress-related genes (NtP5CS and NtLEA5) when dealt with drought stress. These results suggest that MbWRKY1 gene plays a positive regulatory role in drought stress response.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Isolation and functional analysis of MxNAS3 involved in enhanced iron stress tolerance and abnormal flower in transgenic Arabidopsis

Metal trace elements, such as Fe, Zn, and Mn, are necessary micronutrients required by all plants. In this study, the MxNAS3 gene was cloned from Malus xiaojinensis and MxNAS3 was localized in the cytoplasmic membrane. The expression level of MxNAS3 in root and new leaf was higher than in mature leaf and phloem, which was greatly influenced by high and low Fe stresses, IAA and ABA treatments in M. xiaojinensis. Over-expression of MxNAS3 in transgenic Arabidopsis thaliana contributed to enhanced Fe stress tolerance, as well as higher levels of root length, fresh weight, concentrations of chlorophyll, nicotianamine, Fe, Zn, and Mn, especially under high and low Fe stresses. More importantly, it was the first time for us to find that higher expression of MxNAS3 in transgenic A. thaliana contributed to misshappen flowers. Moreover, the MxNAS5-OE A. thaliana had increased expression levels of flowering-related genes (AtYSL1, AtYSL3, AtAFDL, AtAP1, ATMYB21, and AtSAP)