ANTIMICROBIAL ACTIVITY OF CALLYSPONGIA DIFFUSA (MARINE SPONGE) ASSOCIATED ENDOPHYTIC BACTERIA L STRAINS

Objective: To screen the antimicrobial activity Of Callyspongia Diffusa (Marine Sponge) Associated Endophytic Bacterial Strains.Methods: We have isolated endophytic bacterias CDB-1 and CDB-2 from marine sponge Callyspongia diffusa and identified as Pseudomonas taiwanensis strain and Lysinibacillussphaericus strain respectively by the phylogenetic analysis. Fractions of CDB-1 and CDB-2 were screened for in vitro and in vivo antimicrobial activities against pathogenic bacteria and mycobacterium tuberculosis H37 RV strain by Minimum Inhibitory Concentration (MIC) method.Results: The lowest MIC against Kleibesella pnumoniae, Escherichia coli and Enterococcus feacalis was found to be 0.2 µg/ml and 0.4 µg/ml respectively for CDB-2. A significant antifungal activity was observed against Candida albicans (0.2-0.8 µg/ml) and Aspergillus niger (0.2-0.4 µg/ml). Further, Chloroform fraction of CDB-1 and ethyl acetate fraction of CDB-2 have shown significant anti-tubercular activity against the tested organism with MIC of 6.25µg/ml. This was supported by in vivo antimicrobial activity against K. Pneumonia infection in mice and least haemolytic activity against erythrocytes was observed. Compared to chloramphenicol.Conclusion: In this study, we have reported the marine natural species offer a rich source of bioactive metabolites that can exploit to develop novel, useful and potential therapeutic agents

Trans-arterial hepatic radioembolisation of yttrium-90 microspheres

The liver represents a frequent site for metastatic disease, in addition to being a site for primary cancer. Hepatic metastases from certain neoplasms, such as colon, neuroendocrine, melanoma and gastrointestinal stromal tumour have a distinct predilection to metastasize the liver, which in many cases may represent the only or the dominant site of disease. In these circumstances, cytoreduction via surgery or in situ ablative techniques aims to influence the natural history of the disease progression and improve clinical outcomes

ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITE FROM HABENARIA INTERMEDIA D. DON FOR SCREENING OF HEPATOPROTECITVE POTENTIAL AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY IN ALBINO RAT LIVER

The purpose of the study was to isolate the coumarin glycoside constituent from the ethyl acetate fraction of tubers of Habenaria intermedia D. Don by column chromatography using the gradient elution technique. The isolated coumarin glycoside was characterized by spectral studies and screened for hepatoprotecitve potential on CCl4 induced toxicity in rat liver. The coumarin glycoside (Scopoletin) protective activity was evaluated by the assay of liver function biochemical parameters such as SGOT, SGPT, Bilirubin, total protein and histopathological studies of the liver. Results showed that the toxic effect of CCl4 was controlled significantly by restoration of the levels of serum bilirubin, proteins and enzymes as compared to the normal and standard drug Silymarin treated groups. Histology of the liver sections of the rats treated with isolated coumarin glycoside showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further substantiated hepatoprotecitve activity. Therefore, outcome of the present study ascertains that the isolated coumarin glycoside possesses significant hepatoprotecitve activity

Synthesis and properties of liquid-crystal polyesters and polyesteramides

Available from British Library Document Supply Centre- DSC:D86609 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Prospective gated 64 slice computed tomography in assessment of coronary artery disease: A single center experience and relationship of body mass index to outcomes

Coronary artery disease (CAD) is the most common cause of morbidity and mortality in the United States. While Coronary Angiography (CA) is the gold standard test to investigate coronary artery disease, Prospective gated-64 Slice Computed Tomography (Prosp-64CT) is a new non-invasive technology that uses the 64Slice computed tomography (64CT) with electrocardiographic gating to investigate coronary artery disease. The aim of the current study was to investigate the role of Body Mass Index (BMI) as a factor affecting occurrence of CA after a Prosp-64CT, as well as the quality of the Prosp-64CT. Demographic and clinical characteristics of the study population were described. A secondary analysis of data on patients who underwent a Prosp-64CT for evaluation of coronary artery disease was performed. Seventy seven patients who underwent Prosp-64CT for evaluation for coronary artery disease were included. Fifteen patients were excluded because they had missing data regarding BMI, quality of the Prosp-64CT or CA. Thus, a total of 62 patients were included in the final analysis. The mean age was 56.2 years. The mean BMI was 31.3 kg/m 2. Eight (13%) patients underwent a CA within one month of Prosp-64CT. Eight (13%) patients had a poor quality Prosp-64CT. There was significant association of higher BMI as a factor for occurrence of CA post Prosp-64CT (P\u3c0.05). There was a trend, but no statistical significance was observed for the association of being obese and occurrence of CA (P=0.06). BMI, as well as obesity, were not found to be significantly associated with poor quality of Prosp-64CT (P=0.19 and P=0.76, respectively). In conclusion, BMI was significantly associated with occurrence of CA within one month of Prosp-64CT. Thus, in patients with a higher BMI, diagnostic investigation with both tests could be avoided; rather, only a CA could be performed. However, the relationship of BMI to quality of Prosp-64CT needs to be further investigated since the sample size of the current study was small

Assessment of implementation of COTPA-2003 in Bengaluru city, India: A cross-sectional study

Introduction: Tobacco as a slow and modern epidemic remains a serious public health problem for the country. Despite the existence of a comprehensive law to reduce tobacco burden, India still faces the uphill task of its acceptance and successful implementation. Aim: To assess the implementation of Cigarettes and Other Tobacco Products Act 2003 (COTPA)-2003 (Section 4, 6b, and 7) in public places of Bengaluru city and to assess the awareness of the head of these institutions/offices regarding COTPA-2003 and its enforcement in their premises. Materials and Methods: A cross-sectional study was conducted in 175 public places selected as sources of data using cluster random sampling. The tool in the form of a checklist was prepared based on the sections of COTPA-2003 (Cigarettes and Other Tobacco Products' Act), and data were recorded through direct observation. A structured interview was conducted of the institutional heads regarding the implementation of COTPA-2003 in their premises. Informed consent was obtained from the institutional heads or in-charges of the public places. Results: Section 4 (Prohibition of smoking in public places) was not complied by 58%. Only 16.7% educational institutions complied with the Section 6b (Prohibition of sale of tobacco products near educational institutions). More than 50% of the head of the institutions were unaware of their role in the implementation of this law. Conclusion: Although the law has been drafted comprehensively, it is implemented only to a certain extent. Hence, all concerned departments and ministries responsible for meeting the framework convention on tobacco control objectives and enforcing COTPA, at central and state levels, should act urgently and in coordination