12 research outputs found
Highly conductive and pure gold nanostructures grown by electron beam induced deposition
Microstructural analysis and Transport Properties of MoO and MoC nanostructures prepared by focused electron beam-induced deposition
Resonant light scattering from a single dielectric nano-antenna formed by electron beam-induced deposition
The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These receptors interact with the Fc portion of the antibody and are therefore collectively called Fc receptors. Here, using high-speed atomic force microscopy, we observe interactions of human, humanized, and mouse/human-chimeric immunoglobulin G1 (IgG1) antibodies and their cognate Fc receptor, FcγRIIIa. Our results demonstrate that not only Fc but also Fab positively contributes to the interaction with the receptor. Furthermore, hydrogen/deuterium exchange mass spectrometric analysis reveals that the Fab portion of IgG1 is directly involved in its interaction with FcγRIIIa, in addition to the canonical Fc-mediated interaction. By targeting the previously unidentified receptor-interaction sites in IgG-Fab, our findings could inspire therapeutic antibody engineering