Functional properties of enterococcus faecalis isolated from colostrum drawn from thai mothers

International audienceColostrum from Thai mothers who went through vaginal delivery is an abundant source of antibacterial lactic acid bacteria (LAB). Twenty-two strains were isolated and selected according to the antibacterial activity against Staphylococcus aureus TISTR 1466, Escherichia coli TISTR 780, Escherichia coli O157:H7 DMST 12743, Shigella sonnei, Shigella flexneri and Salmonella Typhimurium SA2093. Among the isolates, Enterococcus faecalis was a dominant species. It also exhibited essential probiotic characteristics such as excellent adhesion to mucin and high survival under gut stresses. In addition, the functional characteristic in terms of protein digestibility of colostrum-isolated E faecalis M125 was observed through the hydrolysis of the major allergenic bovine milk proteins. This strain was susceptible to all tested antibiotics, and had no enterococcal virulence factor encoding genes. These results supported that colostrum is a crucial source of beneficial bacteria for infant. E. faecalis M125 poses to be useful as a potential probiotic candidate for the development of functional infant formula

Functional properties of enterococcus faecalis isolated from colostrum drawn from thai mothers

International audienceColostrum from Thai mothers who went through vaginal delivery is an abundant source of antibacterial lactic acid bacteria (LAB). Twenty-two strains were isolated and selected according to the antibacterial activity against Staphylococcus aureus TISTR 1466, Escherichia coli TISTR 780, Escherichia coli O157:H7 DMST 12743, Shigella sonnei, Shigella flexneri and Salmonella Typhimurium SA2093. Among the isolates, Enterococcus faecalis was a dominant species. It also exhibited essential probiotic characteristics such as excellent adhesion to mucin and high survival under gut stresses. In addition, the functional characteristic in terms of protein digestibility of colostrum-isolated E faecalis M125 was observed through the hydrolysis of the major allergenic bovine milk proteins. This strain was susceptible to all tested antibiotics, and had no enterococcal virulence factor encoding genes. These results supported that colostrum is a crucial source of beneficial bacteria for infant. E. faecalis M125 poses to be useful as a potential probiotic candidate for the development of functional infant formula

In vitro three-stage continuous fermentation of gluco-oligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora

Gluco-oligosaccharides produced by Gluconobacter oxydans NCIMB 4943 from maltodextrin as the source, were evaluated for their fermentability by the human colonic microflora. The selectivity of growth of desirable bacteria in the human colon was studied in a three-stage continuous model of the human large intestine. Populations of bacteria, and their fluctuations as a response to the fermentation, were enumerated using fluorescent in situ hybridization (FISH). The gluco-oligosaccharides resulted in increases in numbers of bifidobacteria and the Lactobacillus/Enterococcus group in all 3 vessels of the system, representing the proximal, transverse and distal colonic areas. The prebiotic indices of the glucooligosaccharides were 2.29, 4.23 and 2.74 in V1, V2 and V3 respectively

In vitro fermentation of mixed linkage glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora

The aim of this study was to develop selectively fermented (prebiotic) carbohydrate molecules which would also result in the generation of butyric acid. Glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 from various types of maltodextrins were evaluated for their fermentation by mixed cultures of human colonic microflora. The selectivity of growth of desirable bacteria (bifidobacteria, lactobacilli) was studied in stirred pH-controlled (6.8) batch cultures. Bacterial populations were enumerated using fluorescent in situ hybridization (FISH). Gluco-oligosaccharides resulted in significantly (P<0.05) increased numbers of bifidobacteria and lactobacilli within 24 hours. Bacteroides, clostridial and eubacterial populations were slightly decreased at 48 h. There was very little difference in selectivity between the maltodextrin substrates and the products, although maltodextrin displayed a slightly less selective fermentation than the gluco-oligosaccharide products, also stimulating the growth of bacteroides, clostridia and eubacteria. Gluco-oligosaccharides, produced from G19 maltodextrin, resulted in the best prebiotic effect with the highest prebiotic index (PI) of 5.90 at 48 hours. Acetate, propionate and butyrate were all produced from glucooligosaccharides, derived from G19 maltodextrin, at 48 hours but no lactate or formate were detected