27 research outputs found
Infection of CD8+CD45RO+ Memory T-Cells by HIV-1 and Their Proliferative Response
Get PDFCD8+ T-cells are involved in controlling HIV-1 infection by eliminating infected cells and secreting soluble factors that inhibit viral replication. To investigate the mechanism and significance of infection of CD8+ T-cells by HIV-1 in vitro, we examined the susceptibility of these cells and their subsets to infection. CD8+ T-cells supported greater levels of replication with T-cell tropic strains of HIV-1, though viral production was lower than that observed in CD4+ T-cells. CD8+ T-cell infection was found to be productive through ELISA, RT-PCR and flow cytometric analyses. In addition, the CD8+CD45RO+ memory T-cell population supported higher levels of HIV-1 replication than CD8+CD45RA+ naïve T-cells. However, infection of CD8+CD45RO+ T-cells did not affect their proliferative response to the majority of mitogens tested. We conclude, with numerous lines of evidence detecting and measuring infection of CD8+ T-cells and their subsets, that this cellular target and potential reservoir may be central to HIV-1 pathogenesis
Comparison of stimulating effect on subpopulations of lymphocytes in human peripheral blood by methionine enkephalin with IL-2 and IFN-γ
No full textInvestigation of performance enhancement of solar still incorporated with Gallus gallus domesticus cascara as sensible heat storage material
No full textAbuse of older adults before moving to old age homes in Pokhara Lekhnath Metropolitan City, Nepal: A cross-sectional study
No full textPCR amplification of the hrcV gene through specific primers for detecting Pseudomonas syringae pathovars
No full textCubic-to-inverted micellar and the cubic-to-hexagonal-to-micellar transitions on phytantriol-based cubosomes induced by solvents
No full textApplication of hybrid dimensionality reduction for fault diagnosis of three-phase inverter in PMSM drive system
No full textA two-warehouse inventory model for non-instantaneous deteriorating items with interval-valued inventory costs and stock-dependent demand under inflationary conditions
No full textThis research work develops a two-warehouse inventory model for non-instantaneous deteriorating items with interval-valued inventory costs and stock-dependent demand under inflationary conditions. The proposed inventory model permits shortages, and the backlogging rate is variable and dependent on the waiting time for the next order, and inventory parameters are interval-valued. The main aim of this research is to obtain the retailer’s optimal replenishment policy that minimizes the present worth of total cost per unit time. The optimization problems of the inventory model have been formulated and solved using two variants of particle swarm optimization (PSO) and interval order relations. The efficiency and effectiveness of the inventory model are validated with numerical examples and a sensitivity analysis. The proposed inventory model can assist a decision maker in making important replenishment decisions.</p
