8 research outputs found
Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.
Get PDFIn this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs
Combining micro-RNA and protein sequencing to detect robust biomarkers for Graves’ disease and orbitopathy
No full textMolecular Diagnosis of Urinary Tract Infections by Semi-Quantitative Detection of Uropathogens in a Routine Clinical Hospital Setting
No full textRapid identification of uropathogens by combining Alfred 60 system with matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry technology
No full textMALDI-TOF MS performance compared to direct examination, culture, and 16S rDNA PCR for the rapid diagnosis of bone and joint infections
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