202 research outputs found
Redox regulation of cyanobacterial chimera F1-ATPase comprises interplay between the γ-subunit “neck” region and the turn region of the βDELSEED-loop
Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes
Thiol modulation of the chloroplast ATP synthase γ subunit has been recognized as an important regulatory system for the activation of ATP hydrolysis activity, although the physiological significance of this regulation system remains poorly characterized. Since the membrane potential required by this enzyme to initiate ATP synthesis for the reduced enzyme is lower than that needed for the oxidized form, reduction of this enzyme was interpreted as effective regulation for efficient photophosphorylation. However, no concrete evidence has been obtained to date relating to the timing and mode of chloroplast ATP synthase reduction and oxidation in green plants. In this study, thorough analysis of the redox state of regulatory cysteines of the chloroplast ATP synthase γ subunit in intact chloroplasts and leaves shows that thiol modulation of this enzyme is pivotal in prohibiting futile ATP hydrolysis activity in the dark. However, the physiological importance of efficient ATP synthesis driven by the reduced enzyme in the light could not be demonstrated. In addition, we investigated the significance of the electrochemical proton gradient in reducing the γ subunit by the reduced form of thioredoxin in chloroplasts, providing strong insights into the molecular mechanisms underlying the formation and reduction of the disulfide bond on the γ subunit in vivo. © 2012 The Author
Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin.
The PedR transcriptional regulator interacts with thioredoxin to connect photosynthesis with gene expression in cyanobacteria.
Asymmetry of the three catalytic sites on beta subunits of TF1 from a thermophilic Bacillus strain PS3.
Spatio-Temporal Gene Induction Systems in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp PCC 7120
Determining the Rate-Limiting Step for Light-Responsive Redox Regulation in Chloroplasts
Inverse regulation of F_1_-ATPase activity by a mutation at the regulatory region on the gamma subunit of chloroplast ATP synthase
Structural asymmetry of F1-ATPase caused by the gamma subunit generates a high affinity nucleotide binding site.
- …