Minimal inhibitory concentrations of sulbactam/ampicillin against drug sensitive and drug resistant isolates of Mycobacterium tuberculosis

A total of 92 isolates of Mycobacteriurn tuberculosis consisting of equal numbers of sensitive and resistant strains was tested for their susceptibility to sulbactam and ampicillin (in the ratio of 1:2) on Lowenstein-Jensen (LJ) and 7H11 agar media. The geometric mean MIC was 63.97 μg/ml for the drug sensitive strains and 65.92 μg/ml for the resistant strains, and the overall mean was 65.01 μg/ml. The high MIC on LJ medium could be attributed to the higher protein content which resulted in greater binding of sulbactam/ampicillin. On the other hand, the geometric mean MIC on 7H11 medium was 26.73 μg/ml for sensitive strains and 23.82 μg/ml for resistant strains; the overall mean being 25.23 μg/ml. Although these MlCs of sulbactamampicillin are higher than those reported earlier, they can be easily achieved in serum. Further studies on experimental tuberculosis and in humans will be needed to prove the efficacy of sulbactam/ampicillin in the treatment of patients with multidrug resistant tuberculosis

Evaluation of the BACTEC radiometric method in the early diagnosis of tuberculosis

A comparison of the BACTEC radiometric method with the conventional culture and drug susceptibility testing methods on isolates from clinical specimens in pulmonary and extrapulmonary tuberculosis, childhood TB and TB in HIV-infected individuals was undertaken. In the case of pulmonary TB, the rate of isolation of positive cultures was significantly faster with the BACTEC method, with 87 per cent of the positives being obtained by 7 days, and 96 per cent by 14 days. However, while there was no difference in the total number of positive cultures by the two methods in smear positive pulmonary tuberculosis, in smear negative pulmonary TB, the BACTEC method yielded more number of positive cultures. In extrapulmonary TB, HIV-TB and childhood TB, although the BACTEC method did not yield additional positives, the detection of positives was considerably faster than by the conventional methods, in which the degree of growth was also scanty. The agreement in drug susceptibility tests was 94 per cent for streptomycin and isoniazid, 99 per cent for rifampicin and 91 per cent for ethambutol. Further, most of the drug susceptibility test results became available within 8 days by the BACTEC method. By facilitating early diagnosis, the BACTEC method may prove to be cost effective in a population with a high prevalence of tuberculosis, particularly in the extrapulmonary and paucibacillary forms of the disease

Apoptosis in the chorion laeve of term patients with histologic chorioamnionitis.

OBJECTIVE: The balance between cell survival and cell death (apoptosis) is critical during development and may affect organ function. Apoptosis is accelerated in the presence of infection and inflammation in a variety of organ systems. The objective of this investigation was to determine if apoptosis was increased in the chorion laeve of term patients with and without histologic chorioamnionitis. METHODS: Records of placental pathology were reviewed with respect to the presence/absence of histologic chorioamnionitis. Sections from formalin-fixed, paraffin-embedded fetal membrane rolls were stained using the TUNEL method. The proportion of apoptotic nuclei was calculated in seven high-powered fields/section. Those with and without histologic chorioamnionitis were compared. Data were analyzed using the Mann-Whitney U test, with significance defined as p < 0.05. RESULTS: There was no significant difference in demographic or clinical characteristics between the two groups. The chorion laeve from subjects with histologic chorioamnionitis had significantly more apoptotic nuclei when compared to those without chorioamnionitis (11.2% vs. 5%, p = 0.02). CONCLUSION: Apoptosis is more prevalent in the chorion laeve of fetal membranes with histologic chorioamnionitis. This finding suggests that infection/inflammation may impact cell survival within fetal membranes. The implications of these findings warrant further investigation

Generalized Symmetries of Impulsive Gravitational Waves

We generalize previous \cite{AiBa2} work on the classification of ($C^\infty$) symmetries of plane-fronted waves with an impulsive profile. Due to the specific form of the profile it is possible to extend the group of normal-form-preserving diffeomorphisms to include non-smooth transformations. This extension entails a richer structure of the symmetry algebra generated by the (non-smooth) Killing vectors.Comment: 18 pages, latex2e, no figure

Dependence of the 0.5(2e2/h) conductance plateau on the aspect ratio of InAs quantum point contacts with in-plane side gates

The observation of a 0.5 conductance plateau in asymmetrically biased quantum point contacts with in-plane side gates has been attributed to the onset of spin-polarized current through these structures. For InAs quantum point contacts with the same width but longer channel length, there is roughly a fourfold increase in the range of common sweep voltage applied to the side gates over which the 0.5 conductance plateau is observed when the QPC aspect ratio (ratio of length over width of the narrow portion of the structure) is increased by a factor 3. Non-equilibrium Green s function simulations indicate that the increase in the size of the 0.5 conductance plateau is due to an increased importance, over a larger range of common sweep voltage, of the effects of electron-electron interactions in QPC devices with larger aspect ratio. The use of asymmetrically biased QPCs with in-plane side gates and large aspect ratio could therefore pave the way to build robust spin injectors and detectors for the successful implementation of spin field effect transistorsComment: 30 pages, 9 figure

The interactive effect of water-borne cadmium and environmental hypoxia on common carp (Cyprinus carpio) metabolism

Regarding to the tight association between aquatic hypoxia and heavy metal contaminations in one hand and the role of both parameters on fish respiration, metabolism of carp could be assessed under single and mutual exposures to hypoxia and cadmium. Following measuring LC50-96h of cadmium (43.679 mg/l) for this species, 80 common carp were exposed to 10 different treatments, including control, acute (43.68 mg/l), sub-lethal (21.84 mg/l) and chronic (4.37 mg/l) cadmium as well as hypoxia for immediately (20% of saturation), 24h (40%) and 7 days (60%), and joint exposure of each similar treatment. By using of respirometer technique, we measured oxygen consumption rate in different time spans to calculate each individual standard metabolic rate (SMR), maximum metabolic rate (MMR), aerobic scope (AS), factorial aerobic scope (FAS) and critical oxygen tension (PCrit). Obtained data show that acute and sub-lethal cadmium treatments led to significant (P<0.05) increases in all metabolic indices in comparison with control group whilst the MMR and AS have been reduced (P<0.05) following hypoxia treatments. Combined treatments of hypoxia and cadmium led to reduce SMR and PCrit in all treatments and MMR, AS and FAS only in acute and sub-lethal treatments. In overall, hypoxia can act as a limiting stressor in carp while cadmium can account as a loading stressor

Domino tilings and the six-vertex model at its free fermion point

At the free-fermion point, the six-vertex model with domain wall boundary conditions (DWBC) can be related to the Aztec diamond, a domino tiling problem. We study the mapping on the level of complete statistics for general domains and boundary conditions. This is obtained by associating to both models a set of non-intersecting lines in the Lindstroem-Gessel-Viennot (LGV) scheme. One of the consequence for DWBC is that the boundaries of the ordered phases are described by the Airy process in the thermodynamic limit.Comment: 14 pages, 8 figure