A human endothelial cell-based recycling assay for screening of FcRn targeted molecules.

Albumin and IgG have remarkably long serum half-lives due to pH-dependent FcRn-mediated cellular recycling that rescues both ligands from intracellular degradation. Furthermore, increase in half-lives of IgG and albumin-based therapeutics has the potential to improve their efficacies, but there is a great need for robust methods for screening of relative FcRn-dependent recycling ability. Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. Nat Commun 2018 Feb 12; 9(1):621

Public Private Partnership for Equitable Provision of Quality Health Services

This report presents the findings of an independent Technical Review that focused on the promotion of Public Private Partnership (PPP) for equitable provision of quality health services in Tanzania. The report is meant to contribute to the Annual Joint Health Sector Review 2005. The ToR has been broadly defined, signifying the interest that many stakeholders currently take in PPP. The Review Team (RT), with two international and three national consultants, undertook efforts to consult stakeholders for prioritisation of the issues included in the ToR. The RT had access to a large number of official documents such as laws, by-laws, policy documents, guidelines, etc. and a wide range of studies on PPP set in Tanzania complemented by international literature. The RT undertook efforts to interview relevant persons and committees, associations, organisations, ministries, donors, etc. at the national level as well as in four districts, two rural and two urban. Nevertheless, time constraints did force the RT to limit itself and consequently not all actors that constitute the private health sector could be contacted. It is hoped that the Annual Joint Health Sector Review 2005 will allow a representative participation of all actors in the field of PPP to discuss this review and compensate for any issue or actor that was left out unintended

Infinitely Complex Machines

Infinite machines (IMs) can do supertasks. A supertask is an infinite series of operations done in some finite time. Whether or not our universe contains any IMs, they are worthy of study as upper bounds on finite machines. We introduce IMs and describe some of their physical and psychological aspects. An accelerating Turing machine (an ATM) is a Turing machine that performs every next operation twice as fast. It can carry out infinitely many operations in finite time. Many ATMs can be connected together to form networks of infinitely powerful agents. A network of ATMs can also be thought of as the control system for an infinitely complex robot. We describe a robot with a dense network of ATMs for its retinas, its brain, and its motor controllers. Such a robot can perform psychological supertasks - it can perceive infinitely detailed objects in all their detail; it can formulate infinite plans; it can make infinitely precise movements. An endless hierarchy of IMs might realize a deep notion of intelligent computing everywhere

Production of J/ψJ/\psi-pairs at HERA-N⃗\vec{{\rm N}}

The production of $J/\psi$-pairs as a possible measure of the polarized gluon distribution $\Delta G(x)$ is studied for proton--nucleon collisions at \sqrt{s} =40\;\mbox{GeV}^2 (HERA-$\vec{{\rm N}}$). Possibilities of reconstructing the helicity state of at least one of the $J/\psi$'s are critically reviewed. The observation of production asymmetries in the single polarized mode of HERA-$\vec{{\rm N}}$ is found to be not feasible.Comment: 8 pages, LATeX, 3 figures availabe as .uu-fil

A Multi-variate Discrimination Technique Based on Range-Searching

We present a fast and transparent multi-variate event classification technique, called PDE-RS, which is based on sampling the signal and background densities in a multi-dimensional phase space using range-searching. The employed algorithm is presented in detail and its behaviour is studied with simple toy examples representing basic patterns of problems often encountered in High Energy Physics data analyses. In addition an example relevant for the search for instanton-induced processes in deep-inelastic scattering at HERA is discussed. For all studied examples, the new presented method performs as good as artificial Neural Networks and has furthermore the advantage to need less computation time. This allows to carefully select the best combination of observables which optimally separate the signal and background and for which the simulations describe the data best. Moreover, the systematic and statistical uncertainties can be easily evaluated. The method is therefore a powerful tool to find a small number of signal events in the large data samples expected at future particle colliders.Comment: Submitted to NIM, 18 pages, 8 figure

The beast initiate: the lycanthropy of Heracles

The obscurantist Hellenistic poet Lycophron referenced the initiation of Heracles as a beast suckling the breast of the goddess Hera. This was the event that was the mythological origin of the Galaxy and of the lily flower that incarnated the same deifying essence as the celestial milk of the goddess and it was the etiology for the domestication of felines. As the Lion of Nemea, Heracles was the greatest of the wild cats. The lily was an analogue of a sacred mushroom, as the narkissos of Persephone’s abduction by Hades. The event of the lactation of Heracles is depicted on four Etruscan mirrors and a Faliscan-Hellenic red-figure krater. The deifying milk-flower of the goddess was a ritual of adoption into the family of the celestial deities, that Hera performed also with two other bastard sons of Zeus, Hermes and Dionysus. As the beast being initiated, Heracles became a wolf. Like the motif of the domestication of the cat, the lycanthropy of Heracles involves the whole family of canines, from the domesticated dog to its wilder antecedents in the wolf and its analogue as the fox. The lycanthropy initiation is a bacchanalian rite of root- cutters and is a motif of warrior brotherhood widespread among the Indo-European peoples.Published versio

Capturing Control Room Simulator Data with the HERA Database

The Human Event Repository and Analysis (HERA) system has been developed as a tool for classifying and recording human performance data extracted from primary data sources. This paper reviews the process of extracting data from simulator studies for use in HERA. Simulator studies pose unique data collection challenges, both in types and quality of data measures, but such studies are ideally suited to gather operator performance data, including the full spectrum of performance shaping factors used in a HERA analysis. This paper provides suggestions for obtaining relevant human performance data for a HERA analysis from a control room simulator study and for inputting those data in a format suitable for HERA

Color-Octet Contributions to J/psi Photoproduction via Fragmentation at HERA

We study J/psi photoproduction via fragmentation at next-to-leading order in the QCD-improved parton model, using the nonrelativistic factorization formalism proposed by Bodwin, Braaten, and Lepage. We consider direct and resolved photoproduction of prompt J/psi mesons and chi_{cJ} mesons radiatively decaying to J/psi+gamma, taking into account the formation of both color-singlet and color-octet c anti-c states. Adopting the values of the long-distance color-octet matrix elements extracted from fits to prompt-J/psi data recently taken at the Fermilab Tevatron, we predict that measurements of J/psi photoproduction at DESY HERA should show a distinctive excess over the expectation based on the color-singlet model at small values of the inelasticity variable z. This is complementary to the expected enhancement at z close to 1 due to the color-octet contribution to photon-gluon fusion.Comment: 10 pages (Latex), 3 figures (Postscript