14 research outputs found
Characterisation of self-supporting submicron-thick graphitic carbon foils with polarisation spectroscopy
Proteomics in Cell Culture: From Genomics to Combined âOmics for Cell Line Engineering and Bioprocess Development
Proteomics in Cell Culture: From Genomics to Combined âOmics for Cell Line Engineering and Bioprocess Development
Chemical isotope labeling for quantitative proteomics
Advancements in liquid chromatography and mass spectrometry over the last decades have led to a significant development in mass spectrometry-based proteome quantification approaches. An increasingly attractive strategy is multiplex isotope labeling, which significantly improves the accuracy, precision and throughput of quantitative proteomics in the data-dependent acquisition mode. Isotope labeling-based approaches can be classified into MS1-based and MS2-based quantification. In this review, we give an overview of approaches based on chemical isotope labeling and discuss their principles, benefits, and limitations with the goal to give insights into fundamental questions and provide a useful reference for choosing a method for quantitative proteomics. As a perspective, we discuss the current possibilities and limitations of multiplex, isotope labeling approaches for the data-independent acquisition mode, which is increasing in popularity.</p