Potential of bacteriophage application as an intervention strategy against Salmonella in pigs

Bacteriophage (phage) lysate was used for reducing the dissemination of Salmonella in pigs. The phage lysate was administrated into pigs beginning 1 hour after challenge for 2-hour duration. Three hours postchallenge, pigs were killed and necropsied. The numbers of Salmonella were enumerated in various tissue samples. Administration of the phage lysate lowered the numbers of Salmonella by 2 logs compared with controls in the colon and cecum contents. It suggests that use of phage could be considered as an alternative intervention strategy against Salmonella in pigs at the preharvest stage with further studies

Evaluation of Highly Targeted dsRNA for the Treatment of Infectious Myonecrosis Virus (IMNV) in Litopenaeus vannamei

Double stranded RNA was synthesized in vitro with sequences corresponding to portions of the IMNV genome and were injected into shrimp 2 days after a lethal challenge with IMNV. This is the first time these methods have been used to treat IMNV infections in shrimp

Passive Immunization of Piglets Using Equine Plasma Containing PRRS Virus-Neutralizing Antibodies

Horses were inoculated with several strains of virulent PRRS virus. Sera from the horses were tested for the presence of PRRS virus-neutralizing antibodies. Large volumes of equine plasma were collected and used to passively immunize piglets. Sera were collected at various time points after immunization and tested for virus-neutralizing activity. These results show that horses are capable of generating high neutralizing antibody titers to PRRSV when exposed to virulent virus. Piglets develop neutralizing antibody titers to PRRSV when passively immunized with a sufficient volume of ά-PRRSV equine plasma

The effect of bacteriophage treatment to reduce the rapid dissemination of Salmonella typhimurium in pigs

Bacteriophage treatment significantly reduced the rapid dissemination of Salmonella typhimurium in tonsil and cecum, where the highest number of Salmonella was recovered in pigs experimentally infected with S. typhimurium. The rapid dissemination of Salmonella in market weight pigs prior to slaughter may pose a potential risk in contaminating pork products. Phage treatment should be considered as an intervention strategy to reduce the number of Salmonella in pigs

Molecular Characterization of Multidrug Resistant Salmonella Isolates From a Single Finisher Building for Determination of Horizontal Transmission of Resistance Genes

Salmonella enterica subsp. enterica serovar Typhimurium Phage Type DT104 contains a region where five major antibiotic genes are located, which has been named Salmonella Genomic Island 1 (SGI1) (Boyd, et al, 2002) and bound by Class I integrons which have been shown to aid in transferring genes horizontally. Antibiotic resistance profiles, Pulsed Field Gel Electrophoresis (PFGE) and Polymerase Chain Reaction (PCR) were used to determine if these genes are present in the serovars

Isolation of Salmonella using pooled pen feces from 37 U.S. swine farms

The purpose of this study was to culture the pooled pen feces of market weight pigs to determine the most common Salmonella serotypes being shed and to see if farms positive by culture were also positive by serology. Isolates were analyzed to determine if they had Oantigens capable of inducing an immune response detectable by the Danish Mix-ELISA. There were a total of 286 isolates of Salmonella recovered, 263 of which shared O-antigens with the Mix-ELISA

Direct Delivery of VP19 Double-Stranded RNA into Litopenaeus vannamei by Reverse Gavage Induces Protection against White Spot Syndrome Virus Disease

Double stranded RNA was synthesized in vitro and was delivered by reverse gavage (RG) compared to traditional intramuscular injection (IM) 3 days prior to challenge with a lethal dose of WSSV in both groups

Effect of sodium bicarbonate and sodium bentonite on digestion and rumen fermentation characteristics of forage sorghum silage-based diets fed to growing steers

One percent sodium bicarbonate (NaHCo3) increased intake of a 50% silage - 50% grain diet, but had no effect on intake of a full-feed sorghum silage diet. The addition of concentrate (rolled milo) slightly lowered rumen pH and decreased acid detergent fiber (ADF) and starch digestion. NaHC03 had no effect on digestibility, but 2% bentonite lowered digestibility of NDF and ADF. Neither compound affected rumen fermentation characteristics.; Dairy Day, 1985, Kansas State University, Manhattan, KS, 1985

Injection of Double Stranded RNA Enhances Survival of Litopenaeus vannamei Challenged with White Spot Syndrome Virus

Double stranded RNA was synthesized in vitro with sequences corresponding to portions of the WSSV genome and were injected into shrimp 3 days prior to challenge with a lethal dose of WSSV