17 research outputs found
Rheology of suspensions of hydrophilic and hydrophobic solid particles in nonaqueous media
A note on the avulsion of Ghagghar river in the alluvial plains of Punjab and Haryana, North-West India
Bloodstream infections among children living in rural settings in Uganda and predictors of mortality outcome
Bloodstream infections among children living in rural settings in Uganda and predictors of mortality outcome
The cloning and characterization of a second alpha-amylase of A-hydrophila JMP636
Aims: The aim of this study was to identify, clone and characterize the second amylase of Aeromonas hydrophila JMP636, AmyB, and to compare it to AmyA. Methods and Results: The amylase activity of A. hydrophila JMP636 is encoded by multiple genes. A second genetically distinct amylase gene, amyB, has been cloned and expressed from its own promoter in Escherichia coli. AmyB is a large alpha-amylase of 668 amino acids. Outside the conserved domains of alpha-amylases there is limited sequence relationship between the two alpha-amylases of A. hydrophila JMP636 AmyA and AmyB. Significant (80%) similarity exists between amyB and an alpha-amylase of A. hydrophila strain MCC-1. Differences in either the functional properties or activity under different environmental conditions as possible explanations for multiple copies of amylases in JMP636 is less likely after an examination of several physical properties, with each of the properties being very similar for both enzymes (optimal pH and temperature, heat instability). However the reaction end products and substrate specificity did vary enough to give a possible reason for the two enzymes being present. Both enzymes were confirmed to be alpha-type amylases. Conclusions: AmyB has been isolated, characterized and then compared to AmyA. Significance and Impact of Study: The amylase phenotype is rarely encoded by more than one enzyme within one strain, this study therefore allows the better understanding of the unusual amylase production by A. hydrophila
Experimental determination of core relaxation and screening effects on the wavefunction at a nucleus for stable isotopes of — 151,153Eu II
Performance of the Montreal classification for inflammatory bowel diseases
RESULTS: The inter-observer agreement was excellent for diagnosis (k = 0.96), perianal disease (k = 0.92) and disease location in CD (k = 0.82) and good for age of onset (k = 0.67), upper gastrointestinal disease (k = 0.62), disease behaviour in CD (k = 0.79) and disease extent in UC (k = 0.65). Disease severity in UC was scored poor (k = 0.23). The additional items resulted in a good inter-observer agreement for EIM (k = 0.68) and a moderate agreement for disease severity in CD (k = 0.44). Percentages of correct answers over all Montreal items give a good reflection of the inter-observer agreement (> 80%), except for disease severity (48%-74%). IBD-nurses were significantly worse in scoring upper gastrointestinal disease in CD compared to gastroenterologists (P = 0.008) and gastroenterologists in training (P = 0.040). Observers with less than 10 years of experience were significantly better at scoring UC severity than observers with 10-20 years (P = 0.003) and more than 20 years (P = 0.003) of experience with IBD patient care. Observers with 10-20 years of experience with IBD patient care were significantly better at scoring upper gastrointestinal disease in CD than observers with less than 10 years (P = 0.007) and more than 20 years (P = 0.007) of experience with IBD patient care. CONCLUSION: We found a good to excellent interobserver agreement for all Montreal items except for disease severity in UC (poor). AIM: To validate the Montreal classification system for Crohn's disease (CD) and ulcerative colitis (UC) within the Netherlands. METHODS: A selection of 20 de-identified medical records with an appropriate representation of the inflammatory bowel disease (IBD) sub phenotypes were scored by 30 observers with different professions (gastroenterologist specialist in IBD, gastroenterologist in training and IBD-nurses) and experience level with IBD patient care. Patients were classified according to the Montreal classification. In addition, participants were asked to score extra-intestinal manifestations (EIM) and disease severity in CD based on their clinical judgment. The inter-observer agreement was calculated by percentages of correct answers (answers identical to the "expert evaluation") and Fleiss-kappa (k ). Kappa cutoffs: 0.8 excellent