Государственное регулирование электроэнергетики

Цель работы – разработка теоретических и практических положений, направленных на совершенствование механизмов государственного регулирования электроэнергетики в России, выявление основных тенденций и возможных путей дальнейшего развития названной сферы в российской экономике. Объектом исследования являются отношения, возникающие в процессе осуществления государственного регулирования электроэнергетической отрасли в России.Purpose - to develop theoretical and practical provisions aimed at improving the mechanisms of state regulation of electric power industry in Russia, identifying the main trends and opportunities for further development of the said spheres of the Russian economy. The object of the study are the relations arising in the implementation of state regulation of electric power industry in Russia

3-Methyl-5-isopropylhydantoin as hemoglobin adduct of N,N-dimethylformamide and methylisocyanate [Biomonitoring Methods, 2015]

The analytical method describes the specific determination of 3‐methyl‐5‐isopropylhydantoin (MIH), a hemoglobin adduct of N,N‐dimethylformamide. Following addition of 13C5,15N‐3‐methyl‐5‐isopropylhydantoin (13C5,15N‐MIH) as internal standard, isolated globin is subjected to acid hydrolysis for one hour at 110°C. After pH adjustment (pH 3 – 5) the hydrolysate is extracted twice with ethyl acetate. The combined extracts are washed with sodium hydrogen carbonate solution and dried under a stream of nitrogen. The residue is dissolved in ethyl acetate. An aliquot of the solution is analyzed using GC–MS. For calibration purposes, calibration standards are prepared in pooled globin. They are processed in the same way as the samples to be analyzed

Protein expression of prognostic genes in primary melanoma and benign nevi

Purpose!#!To evaluate the protein expression characteristics of genes employed in a recently introduced prognostic gene expression assay for patients with cutaneous melanoma (CM).!##!Methods!#!We studied 37 patients with CM and 10 with benign (melanocytic) nevi (BN). Immunohistochemistry of primary tumor tissue was performed for eight proteins: COL6A6, DCD, GBP4, KLHL41, KRT9, PIP, SCGB1D2, SCGB2A2.!##!Results!#!The protein expression of most markers investigated was relatively low (e.g., DCD, KRT9, SCGB1D2) and predominantly cytoplasmatic in melanocytes and keratinocytes. COL6A6, GBP4, and KLHL41 expression was significantly enhanced in CM when compared to BN. DCD protein expression was significantly correlated with COL6A6, GBP4, and KLHL41. GBP4 was positively correlated with KLHL41 and inversely correlated with SCGB2B2. The latter was also inversely correlated with serum S100B levels at time of initial diagnosis. The presence of SCGB1D2 expression was significantly associated with ulceration of the primary tumor. KRT9 protein expression was significantly more likely found in acral lentiginous melanoma. The presence of DCD expression was less likely associated with superficial spreading melanoma subtype but significantly associated with non-progressive disease. The absence of SCGB2A2 expression was significantly more often observed in patients who did not progress to stage III or IV.!##!Conclusions!#!The expression levels observed were relatively low but differed in part with those found in BN. Even though we detected some significant correlations between the protein expression levels and clinical parameters (e.g., CM subtype, course of disease), there was no major concordance with the protective or risk-associated functions of the corresponding genes included in a recently introduced prognostic gene expression assay