Oral hyaluronan relieves wrinkles: a double-blinded, placebo-controlled study over a 12-week period

Mariko Oe,1 Seigo Sakai,1 Hideto Yoshida,1 Nao Okado,1 Haruna Kaneda,1 Yasunobu Masuda,1 Osamu Urushibata2 1R&D Division, Kewpie Corporation, Sengawa-cho, Chofu-shi, 2Department of Dermatology, Toho University Ohashi Medical Center, Ohashi, Meguro-ku, Tokyo, Japan Background: Hyaluronan (HA) has critical moisturizing property and high water retention capacity especially for human skin. This study aimed to evaluate the effect of oral intake of HA. Methods: The mean molecular weight (MW) of HA is 2 k and 300 k. Sixty Japanese male and female subjects aged 22–59 years who presented with crow’s feet wrinkles were randomly assigned to the HA 2 k or HA 300 k at 120 mg/day or the placebo group. The subjects were administered HA at a rate of 120 mg/day or a placebo for 12 weeks. The skin wrinkles were evaluated by image analysis of skin wrinkle replicas, and their skin condition was evaluated using a questionnaire survey. Results: During the study period, the HA groups showed better level of the whole sulcus ­volume ratio, wrinkle area ratio, and wrinkle volume ratio than the placebo group. After 8 weeks of ingestion, the HA 300 k group showed significantly diminished wrinkles compared with the placebo group. Skin luster and suppleness significantly improved after 12 weeks in all groups compared with the baseline. Conclusion: The results suggest that oral HA (both HA 2 k and HA 300 k) inhibits skin wrinkles and improves skin condition. Keywords: hyaluronic acid, dietary supplement, skin, wrinkle volume, molecular weigh

Advantages of fluid and white matter suppression (FLAWS) with MP2RAGE compared with double inversion recovery turbo spin echo (DIR-TSE) at 7T.

Cerebrospinal fluid (CSF) and white matter (WM) signal suppression techniques allow better visualization of both WM and gray matter (GM) lesions in such disorders as multiple sclerosis and epilepsy. Recently, a technique, FLuid And White matter Suppression "FLAWS", has been proposed at 3 T based on the magnetization-prepared with two rapid gradient echoes (MP2RAGE) sequence. In this study, the FLAWS-MP2RAGE pulse sequence was compared with a double inversion recovery turbo spin echo (DIR-TSE) sequence at 7 T. Twenty-two healthy volunteers were examined. Isotropic spatial resolution of 1 mm and a scan time of approximately 6 min were chosen due to a restricted clinical schedule. Homogeneity of CSF and WM signal suppression was compared with GM signal as an intensity reference. Volumes of GM visualization and specific absorption rates (SARs) were compared using Wilcoxon-rank sum tests with Bonferroni-Holm correction for multiple comparisons. WM-to-GM signal ratios in FLAWS-MP2RAGE images were significantly lower than DIR-TSE (median: 24.5% vs 59.0%, P <  0.0001), whereas CSF-to-GM signal ratios in FLAWS-MP2RAGE were significantly higher than DIR-TSE (57.1% vs 38.3%, P =  0.0001). Ranges of the signal ratios between 20 and 80 percentiles were lower in FLAWS-MP2RAGE than DIR-TSE for WM (24.1% vs 37.2%, P <  0.0001) but were higher in FLAWS-MP2RAGE compared with DIR-TSE for CSF (80.8% vs 63.0%, P =  0.0001). Pixels of low GM signal (< 20% of the median) were mainly distributed at the skull base, and these low signal GM volume ratios were lower in FLAWS-MP2RAGE than DIR-TSE (2.27% vs 6.18%, P <  0.0001). Median SAR in sixteen subjects was 2.5 times higher in DIR-TSE than in FLAWS-MP2RAGE. FLAWS-MP2RAGE showed superior and more homogenous WM signal suppression, better GM visualization at the skull base and lower SAR compared with DIR-TSE, suggesting superiority of FLAWS-MP2RAGE at 7 T