Effect of CZB medium on the two cell block of preimplantation mouse emryos

This experiment was designed to determine the effects of CZB medium on in vitro culture of murine embryos obtained from FVB/N, CD-1, and CB6F1 strains. Pronuclear stage embryos were recovered from superovulated and mated females 17 h post-hCG. Two-cell stage block, morula and blastocyst stages were used as the end point of the experiments. There is no statistical difference among strains and media for the development to the two-cell stage. However, more FVB/N embryos (88.3 and 82.4%) than CD-1 (57.4 and 57.1%) and CB6F1 (69.8 and 28.9%) reached the 3-8 cell stage in CZB and M16 media, respectively. CB6F1 embryos developed better (69.8%) in CZB than in M16 (28.9%). Morula development for FVB/N embryos was superior (75.0%) in CZB when compared to M16. Collectively, these data suggest that there are strain differences for in vitro embryo development and the in vitro culture medium has a profound effect on in vitro development

Farklı ırktan fare oositlerin in vitro fertilizasyonunda kumulus hücrelerinin etkileri

In this study, the influences of cumulus cells during in vitro fertilization (IVF) and their subsequent effects on mouse embryo development in vitro were studied in inbred and hybrid strain mice. Embryos at the 2-cell stage obtained after in vitro fertilization were transferred to the oviduct of foster mothers to assess their implantation rate. Mouse oocytes, which were intact at the time of in vitro fertilization, demonstrated less polyspermic fertilization and more blastocyst development than those inseminated without any cumulus cells. The data demonstrate that more oocytes were arrested at the 2-cell stage in groups that contained no cumulus cells. There was no statistical difference between cumulus-enclosed and cumulus-free groups for embryo implantation. Data suggest that although there was no difference in terms of implantation rate, more blastocyst stage embryos were obtained from cumulus cell enclosed oocytes. Therefore, we suggest that cumulus cells play many roles during in vitro fertilization, and they should be intact during in vitro fertilization