Combined protein and calcium β-hydroxy-β-methylbutyrate induced gains in leg fat free mass: a double-blinded, placebo-controlled study

Background The leucine metabolite beta-hydroxy-beta-methylbutyrate (HMB) is widely used as an ergogenic supplement to increase resistance-training induced gains in fat free mass (FFM) and strength in healthy adults. Recent studies have questioned the effectiveness of HMB, particularly when a high protein diet is habitually consumed. To investigate the additive resistance-training induced effects of HMB and protein in untrained individuals, we conducted a randomized double-blind, placebo-controlled study that compared the effects of combined protein and HMB supplementation to protein supplementation alone on FFM and muscle strength after 12-week resistance training. Methods Sixteen healthy men (22 +/- 2 yrs) performed a periodized resistance-training program for twelve weeks (four sessions per week). The program comprised two mesocycles, characterized by a linear periodization and non-linear periodization, respectively, and separated by a 1-week tapering period. All participants received 60 g of whey protein on training days and 30 g of whey protein (WP) on non-training days. Participants were randomly assigned to additionally receive 3 g of calcium HMB (WP + HMB) or a placebo (WP + PLA). Body composition and physical fitness were tested before and after the 12-week training program. Whole-body and arm and leg fat free mass (FFM) were assessed by bioimpedance spectroscopy; upper arm and leg fat free cross sectional areas were also quantified using magnetic resonance imaging (MRI); upper and lower body strength were measured by One-repetition maximum (1-RM) bench press and leg press. Results Whole-body and segmental FFM increased in both groups (P < 0.001). However, gains in leg FFM were higher in WP + HMB vs. WP + PLA (arm FFM: + 6.1% vs. + 9.2%, P = 0.2; leg FFM: + 14.2% vs. + 7.0%, P < 0.01). No change in fat mass was observed (P = 0.59). 1-RM increased in both groups (P < 0.001). Conclusions Combined protein and HMB supplementation resulted in segmental, but not whole-body increases in FFM compared to protein supplementation alone. These findings could explain some of the controversial effects of HMB reported in previous studies and have practical implications for maximizing training-induced gains in FFM and clinical conditions associated with skeletal muscle deconditioning such as aging, sedentary lifestyles, bed rest and spaceflight

Gravitational tolerance and size of <i>Brachiosaurus brancai</i>

In an earlier study, Gunga et al. (1999) determined body size and body volume distribution by photogrammetry in sauropods from the Upper Jurassic in Tendaguru (Tanzania, East Africa). Specifically, they found a body mass of about 74400 kg for a specimen of Brachiosaurus brancai. By means of dimensional analysis and a theory of biological similarity, moreover, it was possible to estimate the numerical value of the allometric exponent (b = −0.17) for gravitational tolerance (Gmax) of animals living on earth, which changes with the body mass. This theoretical exponent is close to Economos' empirical finding (b = −0.14). Our results show that there remains an unsolved contradiction between the theoretical assumptions for Gmax for the body mass of the largest fully terrestrial animals. In einer vorangehenden Studie (Gunga et al. 1999) wurde mit Hilfe der Photogrammetrie die Körpermassen und Körpervolumenverteilung von jurassischen Sauropoden aus Tendaguru (Tansania, Ostafrika) ermittelt. Diese Bestimmungen ergaben für Brachiosaurus brancai eine Körpermasse von von ca. 74400 kg. Weitere Studien aus der vergleichenden Physiologie haben gezeigt, dass die Toleranz bei Schwerkraftbelastung (Gmax) mit der Körpermasse variert. Durch dimensionale Analyse und vergleichende Studien konnte ein allometrischer Exponent für die Toleranz bei Schwerkraftbelastung mit b = −0.17 für terrestrische Organismen bestimmt werden. Dieser theoretische Exponent kommt den empirischen Befunden von Economos (b = −0.14) nahe. Dennoch weisen diese vorliegenden Berechnungen damit auf einen Widerspruch bei den bislang vorhandenen theoretischen Überlegungen zur Gmax bei den grössten maximalen Körpermassen für terrestrisch lebende Organismen hin. doi:10.1002/mmng.20020050115</a

The Association of Fatigue With Decreasing Regularity of Locomotion During an Incremental Test in Trained and Untrained Healthy Adults

Fatigue is a key factor that affects human motion and modulates physiology, biochemistry, and performance. Prolonged cyclic human movements (locomotion primarily) are characterized by a regular pattern, and this extended activity can induce fatigue. However, the relationship between fatigue and regularity has not yet been extensively studied. Wearable sensor methodologies can be used to monitor regularity during standardized treadmill tests (e.g., the widely used Bruce test) and to verify the effects of fatigue on locomotion regularity. Our study on 50 healthy adults [27 males and 23 females; &lt;40&nbsp;years; five dropouts; and 22 trained (T) and 23 untrained (U) subjects] showed how locomotion regularity follows a parabolic profile during the incremental test, without exception. At the beginning of the trial, increased walking speed in the absence of fatigue is associated with increased regularity (regularity index, RI, a. u., null/unity value for aperiodic/periodic patterns) up until a peak value (RI&nbsp;=&nbsp;0.909 after 13.8&nbsp;min for T and RI&nbsp;=&nbsp;0.915 after 13.4&nbsp;min for U subjects; median values, n. s.) and which is then generally followed (after 2.8 and 2.5&nbsp;min, respectively, for T/U, n. s.) by the walk-to-run transition (at 12.1&nbsp;min for both T and U, n. s.). Regularity then decreases with increased speed/slope/fatigue. The effect of being trained was associated with significantly higher initial regularity [0.845 (T) vs 0.810 (U), p&nbsp;&lt;&nbsp;0.05 corrected], longer test endurance [23.0&nbsp;min (T) vs 18.6&nbsp;min (U)], and prolonged decay of locomotor regularity [8.6&nbsp;min (T) vs 6.5&nbsp;min (U)]. In conclusion, the monitoring of locomotion regularity can be applied to the Bruce test, resulting in a consistent time profile. There is evidence of a progressive decrease in regularity following the walk-to-run transition, and these features unveil significant differences among healthy trained and untrained adult subjects

Cardiac autonomic modulations and psychological correlates in the Yukon Arctic Ultra : the longest and the coldest ultramarathon

Studies on human physical performance in extreme environments have effectively approached the investigation of adaptation mechanisms and their physiological limits. As scientific interest in the interplay between physiological and psychological aspects of performance is growing, we aimed to investigate cardiac autonomic control, by means of heart rate variability, and psychological correlates, in competitors of a subarctic ultramarathon, taking place over a 690 km course (temperatures between +5 and -47\uc2\ub0C). At baseline (PRE), after 277 km (D1), 383 km (D2), and post-race (POST, 690 km), heart rate (HR) recordings (supine, 15 min), psychometric measurements (Profile of Mood States/POMS, Borg fatigue, and Karolinska Sleepiness Scale scores both upon arrival and departure) were obtained in 16 competitors (12 men, 4 women, 38.6 \uc2\ub1 9.5 years). As not all participants reached the finish line, comparison of finishers (FIN, n = 10) and non-finishers (NON, n = 6), allowed differential assessment of performance. Resting HR increased overall significantly at D1 (FIN +15.9; NON +14.0 bpm), due to a significant decrease in parasympathetic drive. This decrease was in FIN only partially recovered toward POST. In FIN only, baseline HR was negatively correlated with mean velocity [r -0.63 (P.04)] and parasympathetic drive [pNN50+: r -0.67 (P.03)], a lower HR and a higher vagal tone predicting a better performance. Moreover, in FIN, a persistent increase of the long-term self-similarity coefficient, assessed by detrended fluctuation analysis (DFAa2 was retrieved, possibly due to higher alertness. As for psychometrics, at D1, POMS Vigor decreased (FIN: -7.0; NON: -3.8), while Fatigue augmented (FIN: +6.9; NON: +5.0). Sleepiness increased only in NON, while Borg scales did not exhibit changes. Baseline comparison of mood states with normative data for athletes displayed significantly higher positive mood in our athletes. Results show that: the race conditions induced early decreases in parasympathetic drive; the extent of vagal withdrawal, associated to the timing of its recovery, is crucial for success; pre-competition lower resting HR predicts a better performance; psychological profile is reliably depicted by POMS, but not by Borg fatigue scales. Therefore, assessment of heart rate variability and psychological profile may monitor and partly predict performance in long-duration ultramarathon in extreme cold environment

A new body mass estimation of <i>Brachiosaurus brancai</i> Janensch, 1914 mounted and exhibited at the Museum of Natural History (Berlin, Germany)

Body mass and surface areas are important in several aspects for an organism living today. Therefore, mass and surface determinations for extinct dinosaurs could be important for paleo-biological aspects as well. Based on photogrammetrical measurement the body mass and body surface area of the Late Jurassic Brachiosaurus brancai Janensch, 1914 from Tendaguru (East Africa), a skeleton mounted and exhibited at the Museum of Natural History in Berlin (Germany), has been re-evaluated. We determined for a slim type of 3D reconstruction of Brachiosaurus brancai a total volume of 47.9 m3 which represents, assuming a mean tissue density of 0.8 kg per 1,000 cm3, a total body mass of 38,000 kg. The volume distributions from the head to the tail were as follows: 0.2 m3 for the head, neck 7.3 m3, fore limbs 2.9 m3, hind limbs 2.6 m3, thoracic-abdominal cavity 32.4 m3, tail 2.2 m3. The total body surface area was calculated to be 119.1 m2, specifically 1.5 m2 for the head, 26 m2 neck, fore limbs 18.8 m2, hind limbs 16.4 m2, 44.2 m2 thoracic-abdominal cavity, and finally the tail 12.2 m2. Finally, allometric equations were used to estimate presumable organ sizes of this extinct dinosaur and to test whether their dimensions really fit into the thoracic and abdominal cavity of Brachiosaurus brancai if a slim body shape of this sauropod is assumed. doi:10.1002/mmng.200700011</a

Negative Energy Balance Does Not Alter Fat-Free Mass During the Yukon Arctic Ultra—The Longest and the Coldest Ultramarathon

Purpose: The objective of this study was to determine alterations in caloric balance, body composition, metabolites, and cytokines in athletes participating in the Yukon Arctic Ultra.Methods: Ten participants traveling on foot in the 2017 692-km event were recruited for the study. Measurements and samples were obtained at pre-event, 278 km (C1), 384 km (C2), and post-event. Body composition measurements were obtained using bioelectrical impedance analysis. Accelerometer devices were utilized to provide an estimation of caloric expenditure and dietary recalls provided assessments of caloric intake. Blood serum samples were collected, processed, and analyzed using enzyme-linked immunosorbent assays or nuclear magnetic resonance. Results were analyzed using linear mixed model, presented as means ± SD, and considered significant at p &lt; 0.05.Results: Participants (8 males, 2 females; age: 37 ± 10 years; body mass index: 24.4 ± 2.5 kg/m2) were recruited. Four males and one female completed the entire event in 260 ± 19 h. Caloric intake/expenditure was 4,126 ± 1,115 kcal/day and 6,387 ± 781 kcal/day, respectively, indicating a caloric deficit of 2,261 ± 1,543 kcal/day. Total mass, body mass index, and fat mass were reduced at each time point of the event. Fat-free mass (FFM) was unchanged throughout the event. Follistatin was increased at C1 (1,715 ± 876 pg/ml) in comparison to baseline. Acetoacetate increased significantly at post-event (6.1 ± 1.5 mg/ml).Conclusions: Despite a pronounced caloric deficit and sustained activity under extreme cold conditions, FFM was preserved with an increase in serum follistatin and acetoacetate. Future studies should be directed at the role of nutrient strategies and/or training methods on the retention of FFM under these conditions

Анализ технологических параметров работы мембранных систем извлечения гелия из природного газа в процессе разработки газовых месторождений

Объектом исследования являются технологии выделения гелия из состава природного газа. Цель исследования – анализ технологических параметров работы мембранных систем выделения гелия из природного газа в процессе разработки газовых месторождений. В процессе исследования были рассмотрены существующие технологии выделения гелиевого концентрата, проанализированы основные производственные характеристики данных систем. Проведен анализ технологии мембранного выделения гелиевого концентрата и рассмотрены перспективы в использовании современных установок на основе мембранного разделения. В результате проведенного анализа был выявлен положительный эффект от внедрения модульных установок мембранного выделения гелиевого концентрата.The object of the research is technologies for the separation of helium from natural gas. The aim of the study is to analyze the technological parameters of the operation of membrane systems for the extraction of helium from natural gas during the development of gas fields. In the course of the research, the existing technologies for the separation of helium concentrate were considered, the main production characteristics of these systems were analyzed. The analysis of the technology of membrane separation of helium concentrate is carried out and the prospects for the use of modern installations based on membrane separation are considered

Predation upon Hatchling Dinosaurs by a New Snake from the Late Cretaceous of India

A new snake from Upper Cretaceous rocks in India is found with hatchling sauropod dinosaurs, demonstrating that large, gape-limited snakes were probably capable of taking in moderate-sized vertebrate prey