Cystic mesothelioma of the testis in an adolescent patient.

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135297/1/jum2000196423.pd

Isolation and characterization of cellulose degrading ability in Paenibacillus isolates from landfill leachate

Aims: Cellulases are enzymes that convert cellulose into glucose molecules, and are produced by various microorganisms in the environment. Due to their importance to the biofuel industry, there is a need to screen for more efficient varieties of cellulases. In this study, leachate samples from a landfill site were screened for cellulolytic bacteria. Methodology and results: Leachate samples obtained from a landfill collection pond were cultured in an enriched cellulose medium. Two cellulolytic isolates, designated MAEPY1 and MAEPY2, were isolated and further characterized. Phenotypic profiles and phylogenetic analyses using sequences of 16S rRNA, gyrB and whole genome suggested that these isolates are new strains of the Paenibacillus genera. The crude enzyme extracts from both isolates have cellulose degradation activity at approximately 0.1-0.2 IU/mg under working conditions of pH 6 and 55 °C. Assays using other lignocellulosic substrates showed that the crude enzyme extracts also have high xylan degradation activity. Conclusion, significance and impact of study: Paenibacillus sp. are known to produce multiple enzymes for lignocellulolytic degradation and the present results suggest that isolates described in this study, MAEPY1 and MAEPY2, are excellent candidates deserving further study as potential producers of efficient cellulases for use in industries associated with cellulosic biomass

Stress Tensors of Multiparticle Collision Dynamics Fluids

Stress tensors are derived for the multiparticle collision dynamics algorithm, a particle-based mesoscale simulation method for fluctuating fluids, resembling those of atomistic or molecular systems. Systems with periodic boundary conditions as well as fluids confined in a slit are considered. For every case, two equivalent expressions for the tensor are provided, the internal stress tensor, which involves all degrees of freedom of a system, and the external stress, which only includes the interactions with the confining surfaces. In addition, stress tensors for a system with embedded particles are determined. Based on the derived stress tensors, analytical expressions are calculated for the shear viscosity. Simulations illustrate the difference in fluctuations between the various derived expressions and yield very good agreement between the numerical results and the analytically derived expression for the viscosity

A Microcantilever-based Gas Flow Sensor for Flow Rate and Direction Detection

The purpose of this paper is to apply characteristics of residual stress that causes cantilever beams to bend for manufacturing a micro-structured gas flow sensor. This study uses a silicon wafer deposited silicon nitride layers, reassembled the gas flow sensor with four cantilever beams that perpendicular to each other and manufactured piezoresistive structure on each micro-cantilever by MEMS technologies, respectively. When the cantilever beams are formed after etching the silicon wafer, it bends up a little due to the released residual stress induced in the previous fabrication process. As air flows through the sensor upstream and downstream beam deformation was made, thus the airflow direction can be determined through comparing the resistance variation between different cantilever beams. The flow rate can also be measured by calculating the total resistance variations on the four cantilevers.Comment: Submitted on behalf of EDA Publishing Association (http://irevues.inist.fr/handle/2042/16838

Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector.

One of the major hurdles for the development of gene therapy for Fanconi anemia (FA) is the increased sensitivity of FA stem cells to free radical-induced DNA damage during ex vivo culture and manipulation. To minimize this damage, we have developed a brief transduction procedure for lentivirus vector-mediated transduction of hematopoietic progenitor cells from patients with Fanconi anemia complementation group A (FANCA). The lentiviral vector FancA-sW contains the phosphoglycerate kinase promoter, the FANCA cDNA, and a synthetic, safety-modified woodchuck post transcriptional regulatory element (sW). Bone marrow mononuclear cells or purified CD34(+) cells from patients with FANCA were transduced in an overnight culture on recombinant fibronectin peptide CH-296, in low (5%) oxygen, with the reducing agent, N-acetyl-L-cysteine (NAC), and a combination of growth factors, granulocyte colony-stimulating factor (G-CSF), Flt3 ligand, stem cell factor, and thrombopoietin. Transduced cells plated in methylcellulose in hypoxia with NAC showed increased colony formation compared with 21% oxygen without NAC (P<0.03), showed increased resistance to mitomycin C compared with green fluorescent protein (GFP) vector-transduced controls (P<0.007), and increased survival. Thus, combining short transduction and reducing oxidative stress may enhance the viability and engraftment of gene-corrected cells in patients with FANCA

Sum rule for the optical Hall angle

We consider the optical Hall conductivity of a general electronic medium and prove that the optical Hall angle obeys a new sum rule. This sum rule governs the response of an electronic fluid to a Lorentz electric field and can thought of as the transverse counterpart to the f-sum rule in optical conductivity. The physical meaning of this sum rule is discussed, giving a number of examples of its application to a variety of of electronic media.Comment: Four pages. Latex file with two postscript figure

Divergence and Shannon information in genomes

Shannon information (SI) and its special case, divergence, are defined for a DNA sequence in terms of probabilities of chemical words in the sequence and are computed for a set of complete genomes highly diverse in length and composition. We find the following: SI (but not divergence) is inversely proportional to sequence length for a random sequence but is length-independent for genomes; the genomic SI is always greater and, for shorter words and longer sequences, hundreds to thousands times greater than the SI in a random sequence whose length and composition match those of the genome; genomic SIs appear to have word-length dependent universal values. The universality is inferred to be an evolution footprint of a universal mode for genome growth.Comment: 4 pages, 3 tables, 2 figure

The BCS-like gap in superconductor SmFeAsO_0.85F_0.15

Since the discovery of superconductivity in the cuprates two decades ago, it has been firmly established that the CuO_2 plane is consequential for high T_C superconductivity and a host of other very unusual properties. A new family of superconductors with the general composition of LaFeAsO_(1-x)F_x has recently been discovered but with the conspicuous lacking of the CuO_2 planes, thus raising the tantalizing questions of the different pairing mechanisms in these oxypnictide superconductors. Intimately related to pairing in a superconductor are the superconducting gap, its value, structure, and temperature dependence. Here we report the observation of a single gap in the superconductor SmFeAsO_0.85F_0.15 with T_C = 42 K as measured by Andreev spectroscopy. The gap value of 2Delta = 13.34+/-0.3 meV gives 2Delta/k_BT_C = 3.68, close to the BCS prediction of 3.53. The gap decreases with temperature and vanishes at T_C in a manner consistent with the Bardeen-Cooper-Schrieffer (BCS) prediction but dramatically different from that of the pseudogap behavior in the cuprate superconductors. Our results clearly indicate a nodeless gap order parameter, which is nearly isotropic in size across different sections of the Fermi surface, and are not compatible with models involving antiferromagnetic fluctuations, strong correlations, t-J model, and the like, originally designed for cuprates.Comment: 8 pages, 3 figure

Asynchronous control with ATR for large robot teams

In this paper, we discuss and investigate the advantages of an asynchronous display, called "image queue", tested for an urban search and rescue foraging task. The image queue approach mines video data to present the operator with a relevant and comprehensive view of the environment by selecting a small number of images that together cover large portions of the area searched. This asynchronous approach allows operators to search through a large amount of data gathered by autonomous robot teams, and allows comprehensive and scalable displays to obtain a network-centric perspective for unmanned ground vehicles (UGVs). In the reported experiment automatic target recognition (ATR) was used to augment utilities based on visual coverage in selecting imagery for presentation to the operator. In the cued condition a box was drawn in the region in which a possible target was detected. In the no-cue condition no box was drawn although the target detection probability continued to play a role in the selection of imagery. We found that operators using the image queue displays missed fewer victims and relied on teleoperation less often than those using streaming video. Image queue users in the no-cue condition did better in avoiding false alarms and reported lower workload than those in the cued condition. Copyright 2011 by Human Factors and Ergonomics Society, Inc. All rights reserved