16,090 research outputs found
Internal Stresses and Formation of Switchable Nanowires at Thin Silica Film Edge
At vertical edges, thin films of silicon oxide (SiO_{2-x}) contain
semiconductive c-Si layered nanocrystals (Si NC) embedded in and supported by
an insulating g-SiO2 matrix. Tour et al. have shown that a trenched thin film
geometry enables the NC to form switchable nanowires (SNW) when trained by an
applied field. The field required to form SNW decreases rapidly within a few
cycles, or by annealing at 600 C in even fewer cycles, and is stable to 700C.
Here we describe the intrinsic evolution of Si NC and SNW in terms of the
competition between internal stresses and electro-osmosis. The analysis relies
heavily on experimental data from a wide range of thin film studies, and it
explains why a vertical edge across the planar Si-SiOx interface is necessary
to form SNW. The discussion also shows that the formation mechanisms of Si NC
and Si/SiO_{2-x} SNW are intrinsic and result from optimization of nanowire
conductivity in the presence of residual host misfit stresses
Development and application of a self-referencing glucose microsensor for the measurement of glucose consumption by pancreatic ?-cells
Glucose gradients generated by an artificial source and ?-cells were measured using an enzyme-based glucose microsensor, 8-?m tip diameter, as a self-referencing electrode. The technique is based on a difference measurement between two locations in a gradient and thus allows us to obtain real-time flux values with minimal impact of sensor drift or noise. Flux values were derived by incorporation of the measured differential current into Fick's first equation. In an artificial glucose gradient, a flux detection limit of 8.2 ± 0.4 pmol·cm-2·s-1 (mean ± SEM, n = 7) with a sensor sensitivity of 7.0 ± 0.4 pA/mM (mean ± SEM, n = 16) was demonstrated. Under biological conditions, the glucose sensor showed no oxygen dependence with 5 mM glucose in the bulk medium. The addition of catalase to the bulk medium was shown to ameliorate surface-dependent flux distortion close to specimens, suggesting an underlying local accumulation of hydrogen peroxide. Glucose flux from ?-cell clusters, measured in the presence of 5 mM glucose, was 61.7 ± 9.5 fmol·nL-1·s-1 (mean ± SEM, n = 9) and could be pharmacologically modulated. Glucose consumption in response to FCCP (1 ?M) transiently increased, subsequently decreasing to below basal by 93 ± 16 and 56 ± 6%, respectively (mean ± SEM, n = 5). Consumption was decreased after the application of 10 ?M rotenone by 74 ± 5% (mean ± SEM, n = 4). These results demonstrate that an enzyme-based amperometric microsensor can be applied in the self-referencing mode. Further, in obtaining glucose flux measurements from small clusters of cells, these are the first recordings of the real-time dynamic of glucose movements in a biological microenvironment. <br/
Glacial cycles drive variations in the production of oceanic crust
Glacial cycles redistribute water between oceans and continents causing
pressure changes in the upper mantle, with consequences for melting of Earth's
interior. Using Plio-Pleistocene sea-level variations as a forcing function,
theoretical models of mid-ocean ridge dynamics that include melt transport
predict temporal variations in crustal thickness of hundreds of meters. New
bathymetry from the Australian-Antarctic ridge shows significant spectral
energy near the Milankovitch periods of 23, 41, and 100 ky, consistent with
model predictions. These results suggest that abyssal hills, one of the most
common bathymetric features on Earth, record the magmatic response to changes
in sea level. The models and data support a link between glacial cycles at the
surface and mantle melting at depth, recorded in the bathymetric fabric of the
sea floor.Comment: 30 pages, 6 figures (including supplementary information).
Resubmitted to Science on 12 December 201
- …