6 research outputs found
Zwiekszenie swoistosci laboratoryjnej diagnostyki toksokarozy
The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concem: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (≤30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction
Human dirofilariosis in Poland: the first cases of autochthonous infections with Dirofilaria repens
Dirofilaria (Nochtiella) repens Railliet et Henry, 1911 (Nematoda: Onchocercidae) is a subcutaneous parasite of dogs and
other carnivorous animals, with human acting as incidental hosts. D. repens occurs endemically in warm climates on various
continents, in Europe mainly in Mediterranean countries. The aim of this study was to summarize information on human
dirofilariosis in Poland, taking into consideration parasitological and epidemiological data. Between April 2009 – December
2011, in the parasitological laboratories of Medical University in Warsaw and the National Institute of Public Health/National
Institute of Hygiene, fragments of affected human tissues and parasite specimens were examined microscopically. Molecular
methods were used to confirm the results from eight microscopic investigations. A literature review to summarize all data on
dirofilarial infections in humans in Poland was conducted. In these investigations, autochthonous dirofilariosis was found in
humans for the first time in Poland. During the last 3 years, 12 new cases of human D. repens dirofilariosis were recognized.
Since 2007, a total of 18 D. repens infection have been found in humans in Poland. Parasitic changes were located in various
parts of the body, in the form of subcutaneous nodules containing single nematodes surrounded by granulation tissue
(15 cases). In 3 cases, a subconjuctival localization was found. Seventeen of the 18 described cases were noted in central
Poland where dirofilariosis occured in dogs. In this area, autochtonous infection was identified in 3 women who had never
left Poland in their lives; the others were probably infected outside the country while staying in endemic regions. Data on
human and canine infection collected from central Poland during the last 5 years indicates that Dirofilaria repens has been
introduced into our country, and that the infection is successfully spreading, with the border of the endemic area currently
on 52°N, 21°E. To control the epidemiological situation it is necessary to identify D. repens hosts within local mosquito
populations, and to monitor dogs. Because of the increasing number of cases of human infections, whether introduced or
local, physicians should take dirofilariosis into consideration in differential diagnosis of skin and eye diseases