10 research outputs found

    Helicobacter canis colonization in sheep: a zoonotic link

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    Background: Helicobacter canis has been associated with hepatobiliary and gastrointestinal disease in dogs, cats, and humans. Infection has not been documented in other species. Materials and Methods: Sheep feces subjected to microaerobic culture. Isolates were characterized by genus-specific PCR, restriction fragment length polymorphism, biochemical profiling, and 16S rRNA sequence analysis. Results: Helicobacter canis was isolated from sheep feces and confirmed by the above methods. These isolates are distinct from other sheep-origin enterohepatic Helicobacter species previously isolated. Conclusions: This study identifies sheep as H. canis reservoirs potentially important in zoonotic or foodborne transmission. ©2014NIH (grant no. T32 RR007036)NIH (grant no. R01 OD011141)NIH (grant no. P01 CA028842)NIH (grant no. P30 ES02109

    Helicobacter canis colonization in sheep: A zoonotic link

    No full text
    Background: Helicobacter canis has been associated with hepatobiliary and gastrointestinal disease in dogs, cats, and humans. Infection has not been documented in other species. Materials and Methods: Sheep feces subjected to microaerobic culture. Isolates were characterized by genus-specific PCR, restriction fragment length polymorphism, biochemical profiling, and 16S rRNA sequence analysis. Results: Helicobacter canis was isolated from sheep feces and confirmed by the above methods. These isolates are distinct from other sheep-origin enterohepatic Helicobacter species previously isolated. Conclusions: This study identifies sheep as H. canis reservoirs potentially important in zoonotic or foodborne transmission. © 2013 John Wiley & Sons Ltd

    Development of Media for Isolation and Cultivation of Human Cancer Cells

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