The Lantern Vol. 15, No. 3, Summer 1947

• On Sleeping at Lectures • So You Want Security • Mild and Bitters • The Child April • Helgoland • His Majesty, Tabby • January Interval • A Friend or Two • Wish in June • The Search • Jack of 54 and Davey Jones • Song of the Earth • Donald Gay Baker • The Dilemma by the Horns • Psychologyhttps://digitalcommons.ursinus.edu/lantern/1042/thumbnail.jp

Single-Cell Sequencing Reveals Trajectory of Tumor-Infiltrating Lymphocyte States in Pancreatic Cancer

Pancreatic ductal adenocarcinoma (PDAC) has few effective treatments. Immunotherapy, an attractive alternative strategy, remains challenging with the lack of knowledge on the tumor-infiltrating lymphocyte (TIL) landscape in PDAC. To generate a reference of T-cell subpopulations, we profiled 80,000 T cells from 57 PDAC samples, 22 uninvolved/normal samples, and cultured TIL using single-cell transcriptomic and T-cell receptor analysis. These data revealed 20 cell states and heterogeneous distributions of TIL populations. The CD8+ TIL contained a putative transitional GZMK+ population based on T-cell receptor clonotype sharing, and cell-state trajectory analysis showed similarity to a GZMB+PRF1+ cytotoxic and a CXCL13+ dysfunctional population. Statistical analysis suggested that certain TIL states, such as dysfunctional and inhibitory populations, often occurred together. Finally, analysis of cultured TIL revealed that high-frequency clones from effector populations were preferentially expanded. These data provide a framework for understanding the PDAC TIL landscape for future TIL use in immunotherapy for PDAC

Permanent neonatal diabetes mellitus: A case report with plasma insulin studies

SCOPUS: ar.jinfo:eu-repo/semantics/publishe