Prox1-positive cells monitor and sustain the murine intestinal epithelial cholinergic niche

The enteric neurotransmitter acetylcholine governs important intestinal epithelial secretory and immune functions through its actions on epithelial muscarinic Gq-coupled receptors such as M3R. Its role in the regulation of intestinal stem cell function and differentiation, however, has not been clarified. Here, we find that nonselective muscarinic receptor antagonism in mice as well as epithelial-specific ablation of M3R induces a selective expansion of DCLK1-positive tuft cells, suggesting a model of feedback inhibition. Cholinergic blockade reduces Lgr5-positive intestinal stem cell tracing and cell number. In contrast, Prox1-positive endocrine cells appear as primary sensors of cholinergic blockade inducing the expansion of tuft cells, which adopt an enteroendocrine phenotype and contribute to increased mucosal levels of acetylcholine. This compensatory mechanism is lost with acute irradiation injury, resulting in a paucity of tuft cells and acetylcholine production. Thus, enteroendocrine tuft cells appear essential to maintain epithelial homeostasis following modifications of the cholinergic intestinal niche

The Impact of State Nursing Home Staffing Standards on Nurse Staffing Levels

This study investigated the impact of state nursing home staffing standards on nurse staffing levels for the year 2011. Specifically, the study attempted to measure state staffing standards at facility level (i.e., nurse staffing levels that each individual nursing home must retain by its state staffing standards) and analyzed the policy impact. The study findings indicated that state staffing standards for the categories of registered nurse, licensed nurse, or total nurse are positively related to registered nurse, licensed nurse, or total nurse staffing levels, respectively. Nursing homes more actively responded to licensed staffing requirements than total staffing requirements. However, nursing homes did not increase their staffing levels as much as those required by state staffing standards. It is possibly because the quality-oriented inspection allows flexibility in nursing homes\u27 control of nurse staffing levels

The Role of Temporary Agency Work in Different Industrial Relations Systems - a Comparison between Germany and the USA

Although temporary agency work has been growing over the last decade, its use differs widely in different industrial relations systems. Drawing on theoretical and empirical insights, propositions are developed on why companies deploy temporary agency work. These propositions are then contrasted with case-study evidence collected in Germany and the USA to analyse the deployment of agency work in two different industrial relations systems and the role that agency work plays in these diverse settings. The main conclusions of the research are that differences in the deployment of temporary agency workers exist with regard to legal regulation and employers' strategies of labour use while similarities exist regarding the supply of agency labour. Copyright Blackwell Publishing Ltd/London School of Economics 2007.