PHYTOCHEMICAL SCREENING AND ANTIAMEBIC STUDIES OF TAMARINDUS INDICA OF LEAVES EXTRACT

Objective: The present study deals with preliminary phytochemical screening of Tamarindus indica extracts and investigates its antiamebic effect against Entamoeba histolytica in vitro. Methods: E. histolytica was isolated from stools of patients with amebic dysentery and cultivated in lock-egg medium. The leaves extract was added to check its antiamebic activity. Results: The phytochemical screening shows that T. indica contains alkaloids, flavonoids, phenol, and tannins. T. indica extracts (aqueous and ethanolic) were added to culture media E. histolytica. It was observed that E. histolytica count reduced to zero after 72 h and 96 h when 15 mg/ml of aqueous and ethanolic extracts were added, respectively. It is also revealed that there is no cytotoxicity against erythrocytes even when high concentration of plant leaves extract is used. Conclusion: The in vitro sensitivity of T. indica leaves extract against the E. histolytica is established

ANTIOXIDANT EFFECT OF VITAMINS COMBINED A, C, AND E DOSE ON TYPE 2 DIABETIC PATIENTS

Objectives: This study is to investigate the effect of oral Vitamins combined (A, C, and E) with metformin on the levels of fasting blood sugar (FBS), postprandial blood sugar (PPBS), hemoglobin (HbA1c), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in Type 2 diabetic patients. Methods: A total of 32 patients with Type 2 diabetes were divided randomly into A and B groups, i.e., 16 per each group. Group A received metformin only twice a day while Group B received one tablet of Antox and metformin twice a day for 3 months. FBS, PPBS, HbA1c, TC, TG, LDL, and HDL were measured before and after the dose, and the results were analyzed statistically. Results: A significant decreases in FBS, PPBS, HbA1c, TC, TG, and LDL whereas results showed increasing significance in HDL level that seen in the patients of Group B which received Antox and metformin compared to the group which received metformin only. In conclusion, the results indicate that daily consumption of supplementary vitamins with metformin leads to improving blood glucose and lipids in patients with Type 2 diabetes and reducing the risk of complications. Hence, the dose can be used combined Vitamins A, C, and E with metformin in the treatment of Type 2 diabetes by maintaining good glycemic control