Multiscale Biofluidic and Nanobiotechnology Approaches for Treating Sepsis in Extracorporeal Circuits

Infectious diseases and their pandemics periodically attract public interests due to difficulty in treating the patients and the consequent high mortality. Sepsis caused by an imbalanced systemic inflammatory response to infection often leads to organ failure and death. The current therapeutic intervention mainly includes "the sepsis bundles," antibiotics (antibacterial, antiviral, and antifungal), intravenous fluids for resuscitation, and surgery, which have significantly improved the clinical outcomes in past decades; however, the patients with fulminant sepsis are still in desperate need of alternative therapeutic approaches. One of the potential supportive therapies, extracorporeal blood treatment, has emerged and been developed for improving the current therapeutic efficacy. Here, I overview how the treatment of infectious diseases has been assisted with the extracorporeal adjuvant therapy and the potential utility of various nanobiotechnology and microfluidic approaches for developing new auxiliary therapeutic methods

Influence of magnetic viscosity on domain wall dynamics under spin-polarized currents

We present a theoretical study of the influence of magnetic viscosity on current-driven domain wall dynamics. In particular we examine how domain wall depinning transitions, driven by thermal activation, are influenced by the adiabatic and nonadiabatic spin-torques. We find the Arrhenius law that describes the transition rate for activation over a single energy barrier remains applicable under currents but with a current-dependent barrier height. We show that the effective energy barrier is dominated by a linear current dependence under usual experimental conditions, with a variation that depends only on the nonadiabatic spin torque coefficient beta.Comment: 8 pages, 4 figure

Temperature dependence of nonlinear auto-oscillator linewidths: Application to spin-torque nano-oscillators

The temperature dependence of the generation linewidth for an auto-oscillator with a nonlinear frequency shift is calculated. It is shown that the frequency nonlinearity creates a finite correlation time, tau, for the phase fluctuations. In the low-temperature limit in which the spectral linewidth is smaller than 1/tau, the line shape is approximately Lorentzian and the linewidth is linear in temperature. In the opposite high-temperature limit in which the linewidth is larger than 1/tau, the nonlinearity leads to an apparent "inhomogeneous broadening" of the line, which becomes Gaussian in shape and has a square-root dependence on temperature. The results are illustrated for the spin-torque nano-oscillator.Comment: 4 pages, 1 figur

Local Hall effect in hybrid ferromagnetic/semiconductor devices

We have investigated the magnetoresistance of ferromagnet-semiconductor devices in an InAs two-dimensional electron gas system in which the magnetic field has a sinusoidal profile. The magnetoresistance of our device is large. The longitudinal resistance has an additional contribution which is odd in applied magnetic field. It becomes even negative at low temperature where the transport is ballistic. Based on the numerical analysis, we confirmed that our data can be explained in terms of the local Hall effect due to the profile of negative and positive field regions. This device may be useful for future spintronic applications.Comment: 4 pages with 4 fugures. Accepted for publication in Applied Physics Letter

Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris

The ability to engineer proteins by directed evolution requires functional expression of the target polypeptide in a recombinant host suitable for construction and screening libraries of enzyme variants. Bacteria and yeast are preferred, but eukaryotic proteins often fail to express in active form in these cells. We have attempted to resolve this problem by identifying mutations in the target gene that facilitate its functional expression in a given recombinant host. Here we examined expression of HRP in Saccharomyces cerevisiae. Through three rounds of directed evolution by random point mutagenesis and screening, we obtained a 40-fold increase in total HRP activity in the S.cerevisiae culture supernatant compared with wild-type, as measured on ABTS [2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)] (260 units/l/OD_(600)). Genes from wild-type and two high-activity clones were expressed in Pichia pastoris, where the total ABTS activity reached 600 units/l/OD_(600) in shake flasks. The mutants show up to 5.4-fold higher specific activity towards ABTS and 2.3-fold higher specific activity towards guaiacol

Tuning the proximity effect in a superconductor-graphene-superconductor junction

We have tuned in situ the proximity effect in a single graphene layer coupled to two Pt/Ta superconducting electrodes. An annealing current through the device changed the transmission coefficient of the electrode/graphene interface, increasing the probability of multiple Andreev reflections. Repeated annealing steps improved the contact sufficiently for a Josephson current to be induced in graphene.Comment: Accepted for publication in Phys. Rev.