7 research outputs found
Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS
No full textNonlinear Data Alignment for UPLCāMS and HPLCāMS Based Metabolomics: Quantitative Analysis of Endogenous and Exogenous Metabolites in Human Serum
No full textLCāMS in Metabonomics: Optimization of Experimental Conditions for the Analysis of Metabolites in Human Urine
No full textHybrid Feature Detection and Information Accumulation Using High-Resolution LCāMS Metabolomics Data
No full textAn approach for the development and selection of chromatographic methods for high-throughput metabolomic screening of urine by ultra pressure LC-ESI-ToF-MS
No full textSince the components of a sample for open metabolomic analysis are unknown a priori a pragmatic approach to method development has been taken in order to develop and select a chromatographic method suitable for high-throughput open metabolomic screening of urine by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). A total of 848 injections of diluted rat urine were made onto a UPLC-ESI-ToF-MS system using several different gradient profiles and run times to determine a suitable method for analysis of urine from male and female rats. Peak integral and multivariate data analysis were performed to investigate the quality of separation and information obtained from these multiple analyses. A suitable 8 min method was selected and is now used routinely for open profiling metabolomic analyses of urine. The use of a sample-relevant QC mix is also discussed. Ā© Springer Science+Business Media, LLC 2008
