Inhibition of cyclophosphomide mutagenicity using Glycyrrihza glabra root extract

The aims of the studying is using methanolic - water extract of the Glycyrrihza glabra root for inhibition the mutagenicity of anticancer drug by cytogenetic assays in vivo in three interaction between the drug and extract. The result shows that the extract is bioantimutagenesis. The treatment by extract after drug causes increase mitotic index and decrease chromosome aberration and sperm head abnormalities. The thin layer chromatography of the extract show that is contains different polar compounds

Cytotoxic and Genotoxic Effect of Aflatoxin B1

The aims of study is to determine cytogenetic and genotoxic effect of aflatoxin B1 in vivo, cytogenetic tests are used in studying include chromosome malformation, cell proliferation and plast index test in bone marrow cell in Wight albino rats, the results show that toxin causes Varity in chromosome malformation and increased the rate of it, so it increased in cell proliferation and plast index value compare with negative control

The Prevalence of Microalbuminuria in Type 2 Diabetes Mellitus Patients in Al-Husain Hospital in Karbala Province- Iraq

This study is performed to assessment of the long term glycaemic hemoglobin (HbA1c) to determine the prevalence of microalbuminuria and to find the risk factors for developing microalbuminuria and consequence nephropathy in patients with type 2 diabetes. The prevalence of microalbuminuria in our study is high (59%) and the percent of and the risk factors that accompanied microalbuminuria are high blood pressure, elevated fasting blood glucose and poor glycemic Diabetes mellitus (D.M.) is a group of metabolic diseases characterized by hyperglycemia poor glycemic control also high  (92.3%) with control

Total and differential leukocytes count in type 2 diabetes mellitus patients in Iraq

To find the correlation between total and differential leukocytes count and the presence of microalbuminuria in diabetic patients. The results show significant positive correlation between total leukocytes count and microalbuminuria in males and females also there is significant positive correlation between neutrophils count and microalbuminuria in males and females but there is no correlation with other types of leukocytes

Cell Cycle Response to Low Power Laser Irradiation in Jurkat E6.1 T-lymphocyte Cell Line

Low-power laser irradiation (LPLI) effects on cell cycle progression in Jurkat E6.1 T-lymphocyte leukemia (JETL) cells were examined in vitro at 635nm (visible) and 780nm (near infrared) wavelengths. The cells were exposed to an energy density of 9.174 J/cm2, and then examined 24, 48 and 72 hours post-irradiation. Cell cycle analysis by flow cytometry at 24 hours post-irradiation revealed that the three phases (G0/G1, S and G2/M) of cultured JETL cells showed different percentages in LPLI (635nm and 780nm) and unirradiated cultures, but S phase cells were observed with significant increased percentages (55.6 and 55.7%, respectively) compared to controls (37.3%). At 48 hours, again cells at S phase were observed with much higher percentages than control cells (48.2 and 51.5% vs. 29.9%, respectively), and the difference was significant (P ? 0.05). At 72 hours, the S phase cells were also observed with much higher percentages than control cells (33.1 and 32.6% vs. 21.3%, respectively), and the difference was also significant (P ? 0.05). Keywords: Cell cycle, Jurkat E6.1 T-lymphocyte leukemia cell line, Low-power laser irradiation

In vivo study of antimutagenic and antioxidant activity of Glycyrrihza glabra root extract

The studying was aimed to inhibition the mutagenecity and oxidation of genomic DNA by use methanol-water extract of Glycyrrihza glabra root (GL) against anticancer drug cyclophosphomide in rats by use DNA fragmentation test, the extract characterized by thin layer chromatography TLC, and antioxidant activity by use β- carotene spray, the experiment design to use tow single dose of GL and single dose of the drug in three interaction, extract before, with and after the drug to investigate the mechanism of which extract effect inside cell. Four multiple doses for drug and extract to investigate the accumulation effect in DNA the results show that extract is contain of different polar compound and have antioxidant activity, so the extract is bio-antimutagenesis and poor dismutagenesis activity, the extract can inhibit accumulation effect of mutagenecity and oxidation activity of the drug

The Cut-off Values of Triglycerides - Glucose Index for Metabolic Syndrome Associated with Type 2 Diabetes Mellitus

يؤدي حدوث متلازمة الأيض مع داء السكري من النوع 2 الى زيادة شدة المرض والوفيات المرتبطة بكل منهما. يوصى باستخدام مؤشر الجلوكوز الصائم للدهون الثلاثية (مؤشر TyG) كعلامة مفيدة للتنبؤ بمتلازمة التمثيل الغذائي. كان الهدف من هذه الدراسة هو تحديد القيم الحدية لنوع Triglyceride-Glucose لتشخيص متلازمة الأيض في داء السكري من النوع الثاني. تم جمع البيانات من مستشفيات بغداد في الفترة ما بين مايو وديسمبر 2019. وكان عدد المشاركين المؤهلين 424 حيث تم قياس الجلوكوز في الدم في وضع الصيام ، مستوى الدهون ، مستوى HbA1c. فضلا عن قياس ضغط الدم وتم حساب مؤشر Triglyceride-Glucose . تم الحصول على الموافقة الأخلاقية والموافقة المستنيرة .استخدم برنامج SPSS لمعالجة البيانات. أظهر مرضى السكري الذين يعانون من متلازمة الأيض زيادة مستوى مؤشر TyG. ازداد انتشار متلازمة الأيض مع زيادة مؤشر TyG. أظهر مؤشر TyG ارتباطًا كبيرًا مع جميع مكونات متلازمة الأيض. حيث ارتبط طرديا مع مستوى الكاوكوز في الدم ومحيط الخصر ,مستوى الدهون وضغط الدم الانبساطي بينما تناسب عكسيا مع مستوى الكولسترول النافع .مقدار قيمة القطع الأمثل للمؤشر كانت 9.14 ، 9.28 للذكور والإناث على التوالي.نستنج من الدراسة ان مؤشر TyG ملائم للكشف عن متلازمة الأيض في داء السكري من النوع الثاني       The co-occurrence of metabolic syndrome with type 2 diabetes mellitus (T2DM) will potentiate the morbidity and mortality that may be associated with each case. Fasting triglycerides-glucose index (TyG index) has been recommended as a useful marker to predict metabolic syndrome. Our study aimed to introduce gender-specific cut-off values of triglycerides- glucose index   for diagnosing metabolic syndrome associated with type 2 diabetes mellitus. The data were collected from Baghdad hospitals between May - December 2019. The number of eligible participants was 424. National cholesterol education program, Adult Treatment Panel III criteria were used to define metabolic syndrome. Measurement of fasting blood glucose, lipid profile, HbA1c level, blood pressure, and anthropometric were done and the triglyceride-glucose index was calculated. Ethical approval and informed consent were obtained .SPSS was used to analyze the data. Diabetic patients with metabolic syndrome showed an increased level of TyG Index. The prevalence of metabolic syndrome increased with increased TyG index quartiles. The TyG-Index showed significant correlations with all components of metabolic syndrome. The optimal cut-off value revealed 9.14, 9.28 for males and females respectively. In conclusion, TyG index is a good predictor of the presence of MetS in T2DM the TyG index, just measured in one laboratory test, is simple, informative and more suitable for the detection of metabolic syndrome in Iraqi type 2 diabetes mellitus

Effect of Pollutant Water on Some Organs and Blood Parameters in Rats

Water pollution causes dangerous problems in our life. The present study aims to determination effect of chemical polluted water on blood parameter and histological changes in rat organs using Wight albino rats. For this study Hilla fabric plant waste water is used as drinking water to the rats for 30 days. Results show that polluted water causes increased in W.B.Cs count and histopathological changes in liver and kidne

Distribution of some Heavy Metals Pollution Caused byAl- Daura Refinery in the Surrounding Region

Due to the higher increase in the oil industry activities in Iraq, since there are a little information of the environmental status of the areas around the refinery locations, and the concerns of a possible environmental pollution that will cause health and life threats to living organisms, this study was carried out.To understand the status of heavy metals pollution in areas inside and surrounding Al-Daura refinery activities, (17) testing locations were chosen, ten locationsoutsidethe refinery and sevenlocationsinside it.One additional location was chosen in a rural (control) area, in the University of Baghdad, to compare between the heavy metals concentration in the study area and a sample from the rural area not affected by the pollution. The soil samples have been taken from (5 and 60 cm) depth from the top surface of the soil. Three samples were taken from each depth for each location to take an average of results.All the samples of soil were taken during the period from Dec/2010 to Feb/2011.The experimental work has been includes the heavy metals concentrations, such as Zinc, Nickel, Lead and Cadmium, have been measured at each selected depth for each testing locations.The results indicate that the mean concentration of Zn and Ni is (62.4 µg/g) and (100.5 µg/g) respectively and this valuesexceeded the mean allowable value by (Alloway, 1995 [2]; Kabata-Pendias and Pendias, 1992 [6]).The most concentrations of Zn, Ni and Pb, with some exceptions, accumulate at the top soil and decrease with the depth except Cd

Inhibition the mutagenicity of vitamin a by using Olea europaea extract

This study aims to investigate the predisposition of the extraction of the extract of olive leaves (Olea europaea of the family Oleaceae, or the olive family) to frustrate the excretion of the inherent toxic caused by vitamin A (henceforth VA). After being sure that the extraction is inane of any negative effects on the living creature, the following tests are adopted, the mitotic index test of the medulla ossium of the bones, and the chromosomal aberration test. The results showed that toxic dose of vitamin A have cytogenetic effect. It cusses increased in bone marrow cell proliferation and increase in chromosome malformation, the olea leaf extract showed anti-mutagenic activity against the cytogenetic effect of vitamin A it decrees chromosome malformation and kept the Mitotic index in normal level