Effects of Saccharomyces cerevisiae supplementation and anhydrous ammonia treatment of wheat straw on in-situ degradability and, rumen fermentation and growth performance of yearling lambs

The effects of Saccharomyces cerevisiae supplementation (6.6×108 cfu) and anhydrous ammonia treatment (3%) of wheat straw (WS) were investigated on in-situ dry matter (DM) degradability, and on rumen fermentation and growth performance of lambs. Rumen-fistulated Menemen sheep fed a diet with and without live yeast were used to assess the DM degradability characteristics of WS and ammonia-treated wheat straw (WSNH3). Twenty-six yearling Menemen male lambs were fed in four groups. Lambs of control group (WS) received untreated WS without supplemental yeast, whereas other three groups were fed WS treated with anhydrous ammonia (WSNH3 group), untreated WS and yeast (WS+YEAST group) or WS treated with anhydrous ammonia and yeast (WSNH3+YEAST group). Supplemented live yeast (4 g/d) was added in the diet. Lambs were offered untreated or ammonia treated WS ad-libitum and concentrate was fed at 1% of live body weight. The degradability of the water-insoluble (fraction B) was significantly increased by all of the treatment groups. Potential degradability (A+B), effective DM degradability's (pe2, pe5, and pe8) and average daily weight gain increased only in WSNH3+YEAST group (p<0.05). Voluntary DM intake was not increased by the treatments (p>0.05), but voluntary metabolizable energy and crude protein intake were increased by WSNH3 and by WSNH3+YEAST (p<0.05). Average daily rumen pH was not affected by any of the treatments, but average daily NH3-N was significantly higher in the WSNH3 and WSNH3+YEAST groups, and total volatile fatty acids were significantly higher in the WS+YEAST and WSNH3+YEAST groups. In conclusion, the improvement of feed value of WS was better by the combination of ammonia-treatment and yeast supplementation compared to either treatment alone. Copyright © 2015 by Asian-Australasian Journal of Animal Sciences

Effects of anhydrous ammonia treatment and Saccharomyces cerevisiae upplementation on the neutral detergent fiber degradability and crude nutrient igestibility of wheat straw in sheep

This study was conducted to determine the effects of anhydrous ammonia treatment and live yeast (Saccharomyces cerevisiae, Yea-Sacc 1026 Powder, cfu/g = 6.6 x 108) supplementation to wheat straw in Menemen type sheep. In two trials, the wheat straw untreated (WS) and treated with 3% anhydrous ammonia (WSNH3) were used. In the first trial, in situ nylon bag method was used to determine the NDF degradation charecteristics of WS and WSNH3 using three rumen cannulated sheeps fed yeast free diet and with 4 g/sheep/day live yeast supplemented diet. To determine in situ NDF degradation charecteristics of wheat straw plus 4 g/sheep/day live yeast-supplemented (WS+Yeast) and treated with 3% anhydrous ammonia plus live yeast-supplemented (WSNH3+yeast) 15 days adaptation period was applied to the cannulated sheep. In the second trial, in vivo classic digestion method was used to determine the crude nutrient digestibility coefficients by using twelve sheep fed four different diets: basal diet (WS) not treated with anhydrous ammonia, treated with 3% anhydrous ammonia (WSNH3), basal diet plus 4 g/sheep/day live yeast-supplemented (WS+Yeast) and treated with 3% anhydrous ammonia plus 4 g/sheep/day live yeast-supplemented (WSNH3+Yeast). Results showed that the effective NDF degradability (pe8) of WS was significantly improved 2.74%, 4.6% and 6.4% by WS+Yeast, WSNH3 and WSNH3+Yeast, respectively. In vivo DM, OM, apparent CP and EE digestibility coefficients of WS were improved with the WS+Yeast, WSNH3 or WSNH3+Yeast (p<0.05). While NDF digestibility coefficient of WS was non-significantly improved by WS+Yeast and WSNH3 (0.44% and 7.43%, respectively), it was significantly improved 11.22% by WSNH3+Yeast