741 research outputs found
Crystal Structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates
The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. To explain the molecular basis of 3 ' flap substrate recognition and cleavage mechanism by Mus81-Eme1, we determined crystal structures of human Mus81-Eme1 bound to various flap DNA substrates. Mus81-Eme1 undergoes gross substrate-induced conformational changes that reveal two key features: (i) a hydrophobic wedge of Mus81 that separates pre- and post-nick duplex DNA and (ii) a 5 ' end binding pocket that hosts the 5 ' nicked end of post-nick DNA. These features are crucial for comprehensive protein-DNA interaction, sharp bending of the 3 ' flap DNA substrate, and incision strand placement at the active site. While Mus81-Eme1 unexpectedly shares several common features with members of the 5 ' flap nuclease family, the combined structural, biochemical, and biophysical analyses explain why Mus81-Eme1 preferentially cleaves 3 ' flap DNA substrates with 5 ' nicked ends.X11119Ysciescopu
ATP-dependent DNA binding, unwinding, and resection by the Mre11/Rad50 complex
ATP-dependent DNA end recognition and nucleolytic processing are central functions of the Mre11/Rad50 (MR) complex in DNA double-strand break repair. However, it is still unclear how ATP binding and hydrolysis primes the MR function and regulates repair pathway choice in cells. Here, Methanococcus jannaschii MR-ATP gamma S-DNA structure reveals that the partly deformed DNA runs symmetrically across central groove between two ATP gamma S-bound Rad50 nucleotide-binding domains. Duplex DNA cannot access the Mre11 active site in the ATP-free full-length MR complex. ATP hydrolysis drives rotation of the nucleotide-binding domain and induces the DNA melting so that the substrate DNA can access Mre11. Our findings suggest that the ATP hydrolysis-driven conformational changes in both DNA and the MR complex coordinate the melting and endonuclease activity.1120Ysciescopu
Hadronic Structure in the Decay
We report on a study of the invariant mass spectrum of the hadronic system in
the decay tau- -> pi- pi0 nu_tau. This study was performed with data obtained
with the CLEO II detector operating at the CESR e+ e- collider. We present fits
to phenomenological models in which resonance parameters associated with the
rho(770) and rho(1450) mesons are determined. The pi- pi0 spectral function
inferred from the invariant mass spectrum is compared with data on e+ e- -> pi+
pi- as a test of the Conserved Vector Current theorem. We also discuss the
implications of our data with regard to estimates of the hadronic contribution
to the muon anomalous magnetic moment.Comment: 39 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
Study of Charmless Hadronic B Meson Decays to Pseudoscalar-Vector Final States
We report results of searches for charmless hadronic B meson decays to
pseudoscalar(pi^+-,K^+-,Pi^0 or Ks^0)-vector(Rho, K* or Omega) final states.
Using 9.7 million BBbar pairs collected with the CLEO detector, we report first
observation of B^- --> Pi^-Rho^0, B^0 --> Pi^+-Rho^-+ and B^- --> Pi^-Omega,
which are expected to be dominated by hadronic b --> u transitions. The
measured branching fractions are (10.4+3.3-3.4+-2.1)x10^-6,
(27.6+8.4-7.4+-4.2)x10^-6 and (11.3+3.3-2.9+-1.4)x10^-6, respectively.
Branching fraction upper limits are set for all the other decay modes
investigated.Comment: 10 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
Observation of Radiative Leptonic Decay of the Tau Lepton
Using 4.68 fb^{-1} of e^+e^- annihilation data collected with the CLEO II
detector at the Cornell Electron Storage Ring (CESR) we have studied tau
radiative decays tau -> mu nu nu gamma and tau -> e nu nu gamma. For a 10 MeV
minimum photon energy in the tau rest frame, the branching fraction of
radiative tau decay to a muon or electron is measured to be
(3.61+-0.16+-0.35)*10^{-3} or (1.75+-0.06+-0.17)*10^{-2}, respectively. The
branching fractions are in agreement with the Standard Model theoretical
predictions.Comment: 11 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
A Measurement of the Decay Asymmetry Parameters in \Xi_{c}^{0}\to \X^{-}\pi^{+}
Using the CLEO II detector at the Cornell Electron Storage Ring we have
measured the decay asymmetry parameter in the decay . We find , using the world average value of
we obtain . The physically allowed range of a decay
asymmetry parameter is . Our result prefers a negative value:
is at the 90% CL. The central value occupies the
middle of the theoretically expected range but is not yet precise enough to
choose between models.Comment: 10 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
Limit on Tau Neutrino Mass from
From a data sample of 29058
decays observed in the CLEO detector we derive a 95% confidence upper limit on
the tau neutrino mass of 28 MeV.Comment: 17 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLN
Search for Lepton-Flavor-Violating Decays of B Mesons
We have searched a sample of 9.6 million BB-bar events for the
lepton-flavor-violating decays B --> h e^{+-} mu^{-+}, B^+ --> h^- e^+ e^+, B^+
--> h^- e^+ mu^+, and B^+ --> h^- mu^+ mu^+, where h is pi, K, rho, and
K*(892), a total of sixteen modes. We find no evidence for these decays, and
place 90% confidence level upper limits on their branching fractions that range
from 1.0 to 8.3 X 10^{-6}.Comment: 8 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLNS, PRD R
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