A Recombinant Influenza A Virus Expressing Domain III of West Nile Virus Induces Protective Immune Responses against Influenza and West Nile Virus

West Nile virus (WNV) continues to circulate in the USA and forms a threat to the rest of the Western hemisphere. Since methods for the treatment of WNV infections are not available, there is a need for the development of safe and effective vaccines. Here, we describe the construction of a recombinant influenza virus expressing domain III of the WNV glycoprotein E (Flu-NA-DIII) and its evaluation as a WNV vaccine candidate in a mouse model. FLU-NA-DIII-vaccinated mice were protected from severe body weight loss and mortality caused by WNV infection, whereas control mice succumbed to the infection. In addition, it was shown that one subcutaneous immunization with 105 TCID50 Flu-NA-DIII provided 100% protection against challenge. Adoptive transfer experiments demonstrated that protection was mediated by antibodies and CD4+T cells. Furthermore, mice vaccinated with FLU-NA-DIII developed protective influenza virus-specific antibody titers. It was concluded that this vector system might be an attractive platform for the development of bivalent WNV-influenza vaccines

An MILP model of post-combustion carbon capture based on detailed process simulation

This contribution presents an integer-linear model of a post-combustion carbon capture plant featuring discrete sizing, part load operation and dynamic behavior. In particular, the model covers a design space from 200 tCO2/h (size of Petra Nova) down to 7.5 tCO2/h, a part load operation range from 50% to 100%, and a CO2 concentration range from 7.5% to 12%. Starting with detailed, partly rate-based, models in Aspen Plus and deriving linear performance planes thereof allows to bring process model information into a linear system modeling domain for a reasonable range of design specifications. By applying this model to a low-emission energy system design optimization, its practicability and added value could be demonstrated. The simulations show that especially for systems with high non-dispatchable energy generation, the information about the carbon capture plant's dynamic behavior is essential. To fit the scope of this paper, the mathematical formulation of the model is reported in a condensed manner. However, all information required to formulate the model is provided

The Maximal Urethral Pressure at Rest and during Normal Bladder Filling Is Only Determined by the Activity of the Urethral Smooth Musculature in the Female

The aim of this opinion paper is to determine the entities that define the maximal urethral pressure (MUP) during rest and during bladder filling that is needed to guarantee continence in females. For the development of this opinion, the literature was searched for via the Pubmed database and historic sources. Animal studies indicate that the maximal urethral pressure is determined by the smooth muscle activity in the mid-urethra. Additionally, during increased smooth muscle tone development, the largest sympathetic responses are found in the middle part of the urethra. This could be confirmed in human studies that are unable to find striated EMG activity in this area. Moreover, the external urethral striated sphincter is situated at the distal urethra, which is not the area with the highest pressure. The external urethral sphincter only provides additional urethral pressure in situations of exertion and physical activity. From a physics point of view, the phasic pressure of the external striated sphincter at the distal urethra cannot be added to the tonic pressure generated by the smooth muscle in the mid-urethra. The assertion that mid-urethral pressure is the result of different pressure forces around the urethra, including that of the external striated sphincter, is not supported by basic research evidence combined with physical calculation and should therefore be considered a misconception in the field of functional urology

EAU guidelines on neurogenic lower urinary tract dysfunction

Context: Most patients with neurogenic lower urinary tract dysfunction (NLUTD) require life-long care to maintain their quality of life (QoL) and to maximise life expectancy. Objective: To provide a summary of the 2008 version of the European Association of Urology (EAU) guidelines on NLUTD and to assess the effectiveness of currently available diagnostic tools, particularly ultrasound imaging and urodynamics. Evidence acquisition: The recommendations provided in the 2008 EAU guidelines on NLUTD are based on a review of the literature, using online searches of Medline and other source documents published between 2004 and 2007. A level of evidence and/or a grade of recommendation have been assigned to the guidelines where possible. Evidence synthesis: NLUTD encompasses a wide spectrum of pathologies, and patients often require life-long, intensive medical care to maximise their life-expectancy and to maintain their QoL. Treatment must be tailored to the needs of the individual patient and, in many cases, involves a multidisciplinary team of experts. Timely diagnosis and treatment are essential if irreversible deterioration of both the upper and lower Urinary tracts are to be avoided. Therapeutic decisions are made on the basis of a comprehensive medical assessment, including urodynamics to identify the type of dysfunction. Advances in investigative technologies have facilitated the noninvasive and conservative management of patients who have NLUTD. Conclusions: The diagnosis and treatment of NLUTD, which is a highly specialised and complex field involving both urology and medicine, requires up-to-date expert advice to be readily available. The current guidelines are designed to fulfil this need. (C) 2009 European Association of Urology. Published by Elsevier B.V. All rights reserved

Efficient scheme for the shallow water equations on unstructured grids with application to the Continental Shelf

In this paper, a shallow-water flow solver is presented, based on the finite-volume method on unstructured grids The method is suitable for flows that occur in rivers, channels, sewer systems (1D), shallow seas, rivers, overland flow (2D), and estuaries, lakes and shelf breaks (3D). We present an outline of the numerical approach and show three 2D test cases and an application of tidal propagation on the Continental Shelf. The benefits of applying an unstructured grid were explored by creating an efficient model network that aims at keeping the number of grid cells per wavelength constant. The computational speed of our method was compared with that of WAQUA/ TRIWAQ and Delft3D (the commonly used structured shallow-flow solvers in The Netherlands), and comparable performance was found.Hydraulic EngineeringCivil Engineering and Geoscience