Technical Challenges in Evaluating Southern China’s Forage Germplasm Resources

The present status of the collection, conservation and utilisation of the pasture germplasm in tropical and subtropical zones in China is reviewed. The Tropical Pasture Research Centre (TPRC) of Chinese Academy of Tropical Agricultural Sciences (CATAS) has been engaged in this research since the 1940s. A low temperature gene bank, an in vitro plant library and a nursery station have been established. In total, 5890 indigenous fodder materials belonging to 478 species, 161 genera and 12 families have been surveyed and collected in South China; 1130 exotic materials belonging to 87 species, 42 genera of grasses and legumes have been introduced and conserved; 3769 materials from 301 species, 127 genera, 12 families have been conserved in the seed bank; 482 materials belonging to 6 species, 6 genera, 3 families have been propagated in vitro, and 388 materials belonging to 10 species, 8 genera, 3 families grown in the plant preservation nursery. Suggestions are made for developing and utilising southern Chinese grassland resources

360ORB-SLAM: A Visual SLAM System for Panoramic Images with Depth Completion Network

To enhance the performance and effect of AR/VR applications and visual assistance and inspection systems, visual simultaneous localization and mapping (vSLAM) is a fundamental task in computer vision and robotics. However, traditional vSLAM systems are limited by the camera's narrow field-of-view, resulting in challenges such as sparse feature distribution and lack of dense depth information. To overcome these limitations, this paper proposes a 360ORB-SLAM system for panoramic images that combines with a depth completion network. The system extracts feature points from the panoramic image, utilizes a panoramic triangulation module to generate sparse depth information, and employs a depth completion network to obtain a dense panoramic depth map. Experimental results on our novel panoramic dataset constructed based on Carla demonstrate that the proposed method achieves superior scale accuracy compared to existing monocular SLAM methods and effectively addresses the challenges of feature association and scale ambiguity. The integration of the depth completion network enhances system stability and mitigates the impact of dynamic elements on SLAM performance.Comment: 6 pages, 9 figure

Genetic Diversity in the Anthracnose Pathogen Infecting \u3ci\u3eStylosanthes\u3c/i\u3e in Brazil, India and China

This work aimed to determine the genetic diversity of Colletotrichum gloeosporioides infecting Stylosanthes spp. in Brazil, China and India. A total of 132 isolate originating from S. seabrana, S. macrocephala, S. capitata, S. scabra, and S. guianensis were used. Four major genetic groups were identified from an analysis of genetic diversity using selection-neutral DNA markers. Group 1 contained 20 isolates and this may represent a genotype that migrated from the center of diversity in Brazil and Colombia to Australia, Thailand and India. Group 2 consisted of 66 Brazilian isolates and group 3 had 19 isolates from Australia, Burundi, Brazil, China, Colombia, Ivory Coast and Peru. The 27 isolates in group 4 were very diverse with \u3e50% dissimilarity between some isolates. Genetic diversity in Brazil and China was more extensive than in the Indian pathogen population

Forage Grasses and Legumes with Broad Adaptation for Southeast Asia

A wide range of forages is currently being evaluated by farmers and researchers in seven countries in Southeast Asia. Broadly-adapted species have been identified for fodder banks, grazed systems, tree cropping, erosion control and improved fallows. The most promising accessions to date are Stylosanthes guianensis CIAT 184, Brachiaria decumbens cv Basilisk, Brachiaria humidicola cv Tully and CIAT 6133, CIAT 6780, Centrosema pubescens CIAT 15160 and Andropogon gayanus cv Kent and CIAT 621. Other forages that show promise are Paspalum atratum, Arachis pintoi, and Macroptilium gracile cv. Maldonado. Local seed supply and distribution systems are needed to ensure that these species reach their potential on farms

50 years publishing on tropical forages - from Tropical Grasslands and Pasturas Tropicales to Tropical Grasslands-Forrajes Tropicales

Preamble of the Volume 9, Issue

Dissection of crop metabolome responses to nitrogen, phosphorus, potassium, and other nutrient deficiencies

Crop growth and yield often face sophisticated environmental stresses, especially the low availability of mineral nutrients in soils, such as deficiencies of nitrogen, phosphorus, potassium, and others. Thus, it is of great importance to understand the mechanisms of crop response to mineral nutrient deficiencies, as a basis to contribute to genetic improvement and breeding of crop varieties with high nutrient efficiency for sustainable agriculture. With the advent of large-scale omics approaches, the metabolome based on mass spectrometry has been employed as a powerful and useful technique to dissect the biochemical, molecular, and genetic bases of metabolisms in many crops. Numerous metabolites have been demonstrated to play essential roles in plant growth and cellular stress response to nutrient limitations. Therefore, the purpose of this review was to summarize the recent advances in the dissection of crop metabolism responses to deficiencies of mineral nutrients, as well as the underlying adaptive mechanisms. This review is intended to provide insights into and perspectives on developing crop varieties with high nutrient efficiency through metabolite-based crop improvement

The Mechanism and Pathways of Dopamine and Dopamine Agonists in Prolactinomas

Dopamine agonists such as bromocriptine and cabergoline are the predominant treatment drugs for prolactinoma by inhibiting prolactin secretion and shrinking tumor size. However, the pathways of either dopamine or its agonists that lead to the death of cells are incompletely understood and some are even conflicting conclusions. The main aim of this paper is to review the different pathways of dopamine and its agonists in prolactinomas to help to gain a better understanding of their functions and drug resistance mechanisms

A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance

Tropical areas have a large distribution of saline soils and tidal flats with a high salinity level. Salinity stress is a key factor limiting the widespread use of tropical forage such as Stylosanthes guianensis (Aubl.) Sw. This study was designed to screen the salinity tolerance of 84 S. guianensis accessions; In a greenhouse experiment, plants were subjected to Hoagland solution or Hoagland solution with 200 mM NaCl for up to 15 days. Salinity tolerant accession CIAT11365 and salinity sensitive accession FM05-2 were obtained based on withered leaf rate (WLR). Further verification of salinity tolerance in CIAT11365 and FM05-2 with different salinity gradients showed that salinity stress increased WLR and decreased relative chlorophyll content (SPAD), maximum photochemical efficiency of photosystem II (Fv/Fm), and photosynthetic rate (Pn) in FM05-2, but CIAT11365 exhibited lower WLR and higher SPAD, Fv/Fm, and Pn. Leaf RNA-Seq revealed that Ca2+ signal transduction and Na+ transport ability, salinity tolerance-related transcription factors and antioxidant ability, an increase of auxin, and inhibition of cytokinin may play key roles in CIAT11365 response to salinity stress. The results of this study may contribute to our understanding of the molecular mechanism underlying the responses of S. guianensis to salinity stress and also provide important clues for further study and in-depth characterization of salinity resistance breeding candidate genes in S. guianensis

Selection of High Yielding and Anthracnose Resistant \u3ci\u3eStylosanthes\u3c/i\u3e for Brazil, India and China

Resistance to anthracnose, dry matter yield (DMY) and seed yield (SY) was assessed for germplasm and breeding lines of Stylosanthes in Brazil, India and China. Overall, Stylosanthes guianensis produced higher DMY than S. scabra, S. capitata and S. macrocephala at most sites in Brazil. Data from China suggest that there are high yielding anthracnose resistant S. guianensis lines that can reduce the reliance on CIAT 184. S. seabrana might also prove successful. S. seabrana in India produced the highest DMY and SY and it can form nodules with native Bradyrhizobium strains. Regional differences in resistance within accessions stress the importance of targeting germplasm to combat the suite of pathogen races present at a local level

SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis

Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Dsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo