[μ-3-(8-Quinol­yloxy)propanedi­yl]bis­[dicarbon­yl(η5-methoxy­carbonyl­cyclo­penta­dien­yl)molybdenum(III)]

The crystal structure of the title dimolybdenum complex, [Mo2(C12H9NO)(C7H7O2)2(CO)4], has a quasi-tetra­hedral Mo2C2 cluster core attached to one 3-(8-quinol­yloxy)propanediyl (L) and two methoxy­carbonyl­cyclo­penta­dienyl (Cp’) ligands which are coordinated to two Mo atoms: one Mo atom bonds two terminal CO ligands while the other links one terminal and one semi-bridging CO ligand. An intra­molecular C—H⋯N hydrogen bond results in the quinolyl plane of the L ligand approaching and being nearly perpendicular to one of the Cp’ rings [88.09 (12)°]. In the supra­molecular structure, a one-dimensional comb-shaped infinite chain is formed approximately along the crystallographic c axis by a combination of inter­molecular C—H⋯O hydrogen bonds and locally generates a C(6) motif. Finally, pairs of inversion-related comb-shaped chains associate into a new ladder-shaped infinite chain through weak π–π stacking inter­actions between neighbouring quinolyl systems (pyridyl centroid–centroid distance = 3.853 Å)

Research of the active reflector antenna using laser angle metrology system

Active reflector is one of the key technologies for constructing large telescopes, especially for the millimeter/sub-millimeter radio telescopes. This article introduces a new efficient laser angle metrology system for the active reflector antenna of the large radio telescopes, with a plenty of active reflector experiments mainly about the detecting precisions and the maintaining of the surface shape in real time, on the 65-meter radio telescope prototype constructed by Nanjing Institute of Astronomical Optics and Technology (NIAOT). The test results indicate that the accuracy of the surface shape segmenting and maintaining is up to micron dimension, and the time-response can be of the order of minutes. Therefore, it is proved to be workable for the sub-millimeter radio telescopes.Comment: 10 pages, 15 figure

Impacts of recent cultivation on genetic diversity pattern of a medicinal plant, Scutellaria baicalensis (Lamiaceae)

<p>Abstract</p> <p>Background</p> <p>Cultivation of medicinal plants is not only a means for meeting current and future demands for large volume production of plant-based drug and herbal remedies, but also a means of relieving harvest pressure on wild populations. <it>Scutellaria baicalensis </it>Georgi (Huang-qin or Chinese skullcap) is a very important medicinal plant in China. Over the past several decades, wild resource of this species has suffered rapid declines and large-scale cultivation was initiated to meet the increasing demand for its root. However, the genetic impacts of recent cultivation on <it>S. baicalensis </it>have never been evaluated. In this study, the genetic diversity and genetic structure of 28 wild and 22 cultivated populations were estimated using three polymorphic chloroplast fragments. The objectives of this study are to provide baseline data for preserving genetic resource of <it>S. baicalensis </it>and to evaluate the genetic impacts of recent cultivation on medicinal plants, which may be instructive to future cultivation projects of traditional Chinese medicinal plants.</p> <p>Results</p> <p>Thirty-two haplotypes of <it>S. baicalensis </it>(HapA-Y and Hap1-7) were identified when three chloroplast spacers were combined. These haplotypes constituted a shallow gene tree without obvious clusters for cultivated populations, suggesting multiple origins of cultivated <it>S. baicalensis</it>. Cultivated populations (<it>h</it>_T= 0.832) maintained comparable genetic variation with wild populations (<it>h</it>_T= 0.888), indicating a slight genetic bottleneck due to multiple origins of cultivation. However, a substantial amount of rare alleles (10 out of 25 haplotypes within wild populations) lost during the course of <it>S. baicalensis </it>cultivation. The genetic differentiation for cultivated group (<it>G</it>_ST= 0.220) was significantly lower than that of wild group (<it>G</it>_ST= 0.701). Isolation by distance analysis showed that the effect of geographical isolation on genetic structure was significant in wild populations (<it>r </it>= 0.4346, <it>P </it>< 0.0010), but not in cultivated populations (<it>r = </it>0.0599, <it>P </it>= 0.2710). These genetic distribution patterns suggest that a transient cultivation history and the extensive seed change among different geographical areas during the course of <it>S. baicalensis </it>cultivation.</p> <p>Conclusions</p> <p>Although cultivated <it>S. baicalensis </it>maintains comparable genetic diversity relative to wild populations, recent cultivation has still imposed profound impacts on genetic diversity patterns of the cultivated <it>S. baicalensis </it>populations, i.e., the loss of rare alleles and homogenization of cultivated populations. This study suggests that conservation-by-cultivation is an effective means for protecting genetic resources of <it>S. baicalensis</it>, however, the wild resources still need to be protected <it>in situ </it>and the evolutionary consequences of extensive seed exchange mediated by human being should be monitored carefully.</p

Gene Responses to Oxygen Availability in Kluyveromyces lactis: an Insight on the Evolution of the Oxygen-Responding System in Yeast

The whole-genome duplication (WGD) may provide a basis for the emergence of the very characteristic life style of Saccharomyces cerevisiae—its fermentation-oriented physiology and its capacity of growing in anaerobiosis. Indeed, we found an over-representation of oxygen-responding genes in the ohnologs of S. cerevisiae. Many of these duplicated genes are present as aerobic/hypoxic(anaerobic) pairs and form a specialized system responding to changing oxygen availability. HYP2/ANB1 and COX5A/COX5B are such gene pairs, and their unique orthologs in the ‘non-WGD’ Kluyveromyces lactis genome behaved like the aerobic versions of S. cerevisiae. ROX1 encodes a major oxygen-responding regulator in S. cerevisiae. The synteny, structural features and molecular function of putative KlROX1 were shown to be different from that of ROX1. The transition from the K. lactis-type ROX1 to the S. cerevisiae-type ROX1 could link up with the development of anaerobes in the yeast evolution. Bioinformatics and stochastic analyses of the Rox1p-binding site (YYYATTGTTCTC) in the upstream sequences of the S. cerevisiae Rox1p-mediated genes and of the K. lactis orthologs also indicated that K. lactis lacks the specific gene system responding to oxygen limiting environment, which is present in the ‘post-WGD’ genome of S. cerevisiae. These data suggested that the oxygen-responding system was born for the specialized physiology of S. cerevisiae

Biodegradation of tribenuron methyl that is mediated by microbial acidohydrolysis at cell-soil interface

a b s t r a c t Tribenuron methyl (TBM) is a member of the sulfonylurea herbicide family and is widely used in weed control. Due to its phytotoxicity to rotating-crops, concerns on TBM-pollution to soil have been raised. In this study, experimental results indicated that microbial activity played a key role in TBM removal from polluted soil. Twenty-six bacterial strains were isolated and their degradation of TBM was evaluated. Serratia sp. strain BW30 was selected and subjected to further investigation on its degradative mechanism. TBM degradation by strain BW30 was dependent on glucose that was converted into lactic or oxalic acids. HPLC-MS analysis revealed two end-products from TBM degradation, and they were identical to the products from TBM acidohydrolysis. Based on this observation, it is proposed that microbemediated acidohydrolysis of TBM was involved in TBM degradation in soil, and possible application of this observation in bioremediation of TBM-polluted soil is discussed