Construction and Characterization of a Novel Fusion Protein MG7-scFv/SEB against Gastric Cancer

Antibody-targeted superantigen has been developed into a new strategy to treat many malignant tumors. In this study, for specific targeting to gastric cancer cell, superantigen SEB (Staphylococcal Enterotoxin B) was genetically fused to the single-chain variable fragment of gastric carcinoma-associated antibody MG7(MG7-scFv) that recognizes the MG7 antigen frequently expressed in gastric cancer cell. The recombinant MG7-scFv/SEB fusion proteins are expressed in E. coli as inclusion bodies, and the purified MG7-scFv/SEB retains high binding affinity with gastric cancer cell SGC-7901 (positive MG7 antigen expression). When incubated with effector cell—peripheral blood mononuclear cells (PBMCs), MG7-scFv/SEB could effectively inhibit the proliferation and induce apoptosis of SGC-7901. After being treated with MG7-scFv/SEB, PBMCs remarkably increased the production of Th1 cytokines (IFN-gamma, IL-2), and slightly increased the production of Th2 cytokines (IL-4, IL-10) in vitro. It was observed that gastric-tumor-bearing rats administrated with MG7-scFv/SEB showed more inflammatory cell infiltration, more significant tumor inhibition, and longer survival time than those of rats treated with SEB or NS (Normal Saline). The data indicated that MG7-scFv/SEB fusion protein could specifically target gastric cancer cell, enhance the activity of T cells and induce tumor cell apoptosis to exert the antitumor effect on gastric cancer

Research progress of computer analysis method of SPECT-CT and PET-CT

多模态成像设备的发展是影像学发展的里程碑，继PET-CT后，美国通用电气公司推出了Discovery NM/CT 670，将CT与SPECT有机整合在一起，形成的SPECT/CT新型分子医学影像设备，随着SPECT-CT和PET-CT日益广泛地受到关注和应用，许多关于SPECT-CT和PET-CT图像分析的计算机方法应运而生，越来越受到临床和影像学科的重视。本文就SPECT-CT和PET-CT的计算机分析方法进行了详细的描述。The development of multi-modality imaging devices marks a milestone of medical imaging. Discovery NM/CT 670 was recently launched by Ge Corp in the United States after the development of PET-CT and it integrated CT and SPECT. The newly formed type of molecular imaging equipment of SPECT/CT is getting wide attention along with the application of SPECT-CT and PET-CT so that computer analysis of SPECT-CT and PET-CT image came into being, which was much valued by clinical and imaging studies. The paper described detailed information of SPECT-CT and PET-CT computer analysis

SPECT-CT和PET-CT计算机分析方法的探索研究

The development of multimodal imaging equipment is milestone in the development of imaging, after the PET-CT, American GE company launched a Discovery 670 NM/CT, CT and SPECT, the organic integration of the formation of SPECT-CT new molecular medical imaging equipment, with SPECT, CT and PET-CT is getting more and more widely attention and application, many of SPECT-CT and PET-CT image analysis computer method arises at the historic moment, getting increasing attention of the clinical and imaging science. The paper carried on the detailed description of the SPECT-CT and PET-CT computer analysis method.多模态成像设备的发展是影像学发展的里程碑，继PET-CT后，美国通用电气公司推出了Discovery NM/CT 670，将CT与SPECT有机整合在一起，形成的SPECT-CT新型分子医学影像设备，随着SPECT-CT和PET-CT日益广泛地受到关注和应用，许多关于SPECT-CT和PET-CT图像分析的计算机方法应运而生，越来越受到临床和影像学科的重视。本文就SPECT/CT和PET-CT的计算机分析方法进行了详细的描述

Robust Finite-Time Control for Spacecraft with Coupled Translation and Attitude Dynamics

Robust finite-time control for spacecraft with coupled translation and attitude dynamics is investigated in the paper. An error-based spacecraft motion model in six-degree-of-freedom is firstly developed. Then a finite-time controller based on nonsingular terminal sliding mode control technique is proposed to achieve translation and attitude maneuvers in the presence of model uncertainties and environmental perturbations. A finite-time observer is designed and a modified controller is then proposed to deal with uncertainties and perturbations and alleviate chattering. Numerical simulations are finally provided to illustrate the performance of the proposed controllers

Robust Finite-Time Control for Spacecraft with Coupled Translation and Attitude Dynamics

Robust finite-time control for spacecraft with coupled translation and attitude dynamics is investigated in the paper. An error-based spacecraft motion model in six-degree-of-freedom is firstly developed. Then a finite-time controller based on nonsingular terminal sliding mode control technique is proposed to achieve translation and attitude maneuvers in the presence of model uncertainties and environmental perturbations. A finite-time observer is designed and a modified controller is then proposed to deal with uncertainties and perturbations and alleviate chattering. Numerical simulations are finally provided to illustrate the performance of the proposed controllers