A bike sharing system based on Blockchain platform

Leakage of user privacy and vandalism of the sharing bike have been the most serious problem since sharing bike came on the scene. Accordingly, it is very urgent to rebuild the underlying trust mechanism. Most bike sharing systems are centralized, leading to overpressure on the central server. This paper proposes a bike sharing system based on blockchain service platform and a shared operation mode of C2C. The system uses the blockchain system as the trust guarantee. The extra chain payment - lightning network is used to improve the efficiency of the blockchain system and the smart contract is used to provide the rights and interests of the two parties

A bike sharing system based on Blockchain platform

Leakage of user privacy and vandalism of the sharing bike have been the most serious problem since sharing bike came on the scene. Accordingly, it is very urgent to rebuild the underlying trust mechanism. Most bike sharing systems are centralized, leading to overpressure on the central server. This paper proposes a bike sharing system based on blockchain service platform and a shared operation mode of C2C. The system uses the blockchain system as the trust guarantee. The extra chain payment - lightning network is used to improve the efficiency of the blockchain system and the smart contract is used to provide the rights and interests of the two parties

Preparation of Special Wettability Quartz Sand Filter Media and Its Synchronous Oil/Water Mixture Separation and Dye Adsorption

To efficiently and synchronously separate oil/water mixture and adsorbed dyes, corn-cob-covered quartz sand (CCQS) filter media with underwater superoleophobic qualities and underoil extremely hydrophobic qualities were fabricated by grafting a corn cob onto the surface of quartz sand using the dip-coating technique. Due to the introduction of more hydrogen bonds on the quartz surface and the construction of a rough structure, the underwater oil contact angles and underoil water contact angles of the CCQS were 150.3~154.6° and 132.2°~154.6°, respectively. A separator for oil/water separation was devised, and the CCQS-filled separator could synchronously separate the oil/water mixture and adsorb malachite green. The separation efficiency of the oil/water mixture was over 99.93%, the removal rate of MG was 99.73%, and the adsorption capacity was 7.28 mg/g. The CCQS could keep its wettability steady under challenging environmental circumstances. Therefore, the study offered a novel concept for the successful oil/water mixture separation, while synchronously adsorbing dye

Preparation of Special Wettability Quartz Sand Filter Media and Its Synchronous Oil/Water Mixture Separation and Dye Adsorption

To efficiently and synchronously separate oil/water mixture and adsorbed dyes, corn-cob-covered quartz sand (CCQS) filter media with underwater superoleophobic qualities and underoil extremely hydrophobic qualities were fabricated by grafting a corn cob onto the surface of quartz sand using the dip-coating technique. Due to the introduction of more hydrogen bonds on the quartz surface and the construction of a rough structure, the underwater oil contact angles and underoil water contact angles of the CCQS were 150.3~154.6° and 132.2°~154.6°, respectively. A separator for oil/water separation was devised, and the CCQS-filled separator could synchronously separate the oil/water mixture and adsorb malachite green. The separation efficiency of the oil/water mixture was over 99.93%, the removal rate of MG was 99.73%, and the adsorption capacity was 7.28 mg/g. The CCQS could keep its wettability steady under challenging environmental circumstances. Therefore, the study offered a novel concept for the successful oil/water mixture separation, while synchronously adsorbing dye

Active microbial population dynamics and life strategies drive the enhanced carbon use efficiency in high-organic matter soils

ABSTRACTMicrobial carbon use efficiency (CUE) is a critical parameter that controls carbon storage in soil, but many uncertainties remain concerning adaptations of microbial communities to long-term fertilization that impact CUE. Based on H218O quantitative stable isotope probing coupled with metagenomic sequencing, we disentangled the roles of active microbial population dynamics and life strategies for CUE in soils after a long-term (35 years) mineral or organic fertilization. We found that the soils rich in organic matter supported high microbial CUE, indicating a more efficient microbial biomass formation and a greater carbon sequestration potential. Organic fertilizers supported active microbial communities characterized by high diversity and a relative increase in net growth rate, as well as an anabolic-biased carbon cycling, which likely explains the observed enhanced CUE. Overall, these results highlight the role of population dynamics and life strategies in understanding and predicting microbial CUE and sequestration in soil.IMPORTANCEMicrobial CUE is a major determinant of global soil organic carbon storage. Understanding the microbial processes underlying CUE can help to maintain soil sustainable productivity and mitigate climate change. Our findings indicated that active microbial communities, adapted to long-term organic fertilization, exhibited a relative increase in net growth rate and a preference for anabolic carbon cycling when compared to those subjected to chemical fertilization. These shifts in population dynamics and life strategies led the active microbes to allocate more carbon to biomass production rather than cellular respiration. Consequently, the more fertile soils may harbor a greater microbially mediated carbon sequestration potential. This finding is of great importance for manipulating microorganisms to increase soil C sequestration

Reproductive organ and young tissues show constrained elemental composition in Arabidopsis thaliana

Background and Aims The identification of stoichiometric homeostasis is crucial for understanding plant adaptive strategies under a changing environment. However, current knowledge of plant stoichiometric homeostasis has mainly been obtained from mature leaves, with little from other organs across different developmental stages. Methods We conducted a greenhouse nitrogen (N) and phosphorus (P) addition experiment to evaluate the strength of stoichiometric homeostasis across different organs and developmental stages of Arabidopsis thaliana. Key Results Homeostatic regulation coefficients (H) for N (H-N), P (H-P) and N : P ratio (H-NP) were highest in reproductive tissue, followed by stem and leaf at the same stage. All H parameters in the same organ decreased significantly over the developmental stages. Leaf H-N, H-P and H-NP were highest at stage 1, followed by stages 2 and 3. Both stem and silique at stage 2 relative to stage 3 had higher H-N, H-P and H-NP. These results suggested that reproductive tissue relative to other organs and young tissue relative to old tissue showed more constrained elemental composition in response to nutrient availabilities, and such trends were also evidenced by stoichiometric scaling relationships. Conclusions Our findings highlight that stoichiometric homeostasis is tightly related to the ontogenesis of plant tissue. These results could have a strong implication for diagnosing relative availabilities of N and P in ecosystems, suggesting that the N and P stoichiometry of old tissues might be stronger indicators of nutrient status for plants, but further study is needed to test the generality across species with more distinguishable functional traits

A method for Absolute Protein Expression Quantity Measurement Employing Insulator RiboJ

Measuring the absolute protein expression quantity for a specific promoter is necessary in the fields of both molecular biology and synthetic biology. The strength of a promoter is traditionally characterized by measuring the fluorescent intensity of the fluorescent protein downstream of the promoter. Until now, measurement of the absolute protein expression quantity for a promoter, however, has been unsuccessful in synthetic biology. The fact that the protein coding sequence influences the expression level for different proteins, and the inconvenience of measuring the absolute protein expression level, present a challenge to absolute quantitative measurement. Here, we introduce a new method that combines the insulator RiboJ with the standard fluorescence curve in order to measure the absolute protein expression quantity quickly; this method has been validated by modeling verification. Using this method, we successfully measured nine constitutive promoters in the Anderson promoter family. Our method provides data with higher accuracy for pathway design and is a straightforward way to standardize the strength of different promoters. Keywords: RiboJ, Promoter measurement, Synthetic biolog

Reproductive organ and young tissues show constrained elemental composition in Arabidopsis thaliana

Background and Aims The identification of stoichiometric homeostasis is crucial for understanding plant adaptive strategies under a changing environment. However, current knowledge of plant stoichiometric homeostasis has mainly been obtained from mature leaves, with little from other organs across different developmental stages. Methods We conducted a greenhouse nitrogen (N) and phosphorus (P) addition experiment to evaluate the strength of stoichiometric homeostasis across different organs and developmental stages of Arabidopsis thaliana. Key Results Homeostatic regulation coefficients (H) for N (H-N), P (H-P) and N : P ratio (H-NP) were highest in reproductive tissue, followed by stem and leaf at the same stage. All H parameters in the same organ decreased significantly over the developmental stages. Leaf H-N, H-P and H-NP were highest at stage 1, followed by stages 2 and 3. Both stem and silique at stage 2 relative to stage 3 had higher H-N, H-P and H-NP. These results suggested that reproductive tissue relative to other organs and young tissue relative to old tissue showed more constrained elemental composition in response to nutrient availabilities, and such trends were also evidenced by stoichiometric scaling relationships. Conclusions Our findings highlight that stoichiometric homeostasis is tightly related to the ontogenesis of plant tissue. These results could have a strong implication for diagnosing relative availabilities of N and P in ecosystems, suggesting that the N and P stoichiometry of old tissues might be stronger indicators of nutrient status for plants, but further study is needed to test the generality across species with more distinguishable functional traits.National Natural Science Foundation of China [31321061, 31330012, 41173083]; National Science Foundation for Fostering Talents in Basic Research [J1103406, J0105]SCI(E)[email protected]

WGCNA Reveals Hub Genes and Key Gene Regulatory Pathways of the Response of Soybean to Infection by <i>Soybean mosaic virus</i>

Soybean mosaic virus (SMV) is one of the main pathogens that can negatively affect soybean production and quality. To study the gene regulatory network of soybeans in response to SMV SC15, the resistant line X149 and susceptible line X97 were subjected to transcriptome analysis at 0, 2, 8, 12, 24, and 48 h post-inoculation (hpi). Differential expression analysis revealed that 10,190 differentially expressed genes (DEGs) responded to SC15 infection. Weighted gene co-expression network analysis (WGCNA) was performed to identify highly related resistance gene modules; in total, eight modules, including 2256 DEGs, were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of 2256 DEGs revealed that the genes significantly clustered into resistance-related pathways, such as the plant–pathogen interaction pathway, mitogen-activated protein kinases (MAPK) signaling pathway, and plant hormone signal transduction pathway. Among these pathways, we found that the flg22, Ca2+, hydrogen peroxide (H2O2), and abscisic acid (ABA) regulatory pathways were fully covered by 36 DEGs. Among the 36 DEGs, the gene Glyma.01G225100 (protein phosphatase 2C, PP2C) in the ABA regulatory pathway, the gene Glyma.16G031900 (WRKY transcription factor 22, WRKY22) in Ca2+ and H2O2 regulatory pathways, and the gene Glyma.04G175300 (calcium-dependent protein kinase, CDPK) in Ca2+ regulatory pathways were highly connected hub genes. These results indicate that the resistance of X149 to SC15 may depend on the positive regulation of flg22, Ca2+, H2O2, and ABA regulatory pathways. Our study further showed that superoxide dismutase (SOD) activity, H2O2 content, and catalase (CAT) and peroxidase (POD) activities were significantly up-regulated in the resistant line X149 compared with those in 0 hpi. This finding indicates that the H2O2 regulatory pathway might be dependent on flg22- and Ca2+-pathway-induced ROS generation. In addition, two hub genes, Glyma.07G190100 (encoding F-box protein) and Glyma.12G185400 (encoding calmodulin-like proteins, CMLs), were also identified and they could positively regulate X149 resistance. This study provides pathways for further investigation of SMV resistance mechanisms in soybean