Research on basis of reverse genetics system of a Sindbis-like virus XJ-160

As a Sindbis-like virus (SINLV), XJ-160 virus was isolated from a pooled sample of Anopheles mosquitoes collected in Xinjiang, China, in 1990. Recombinant plasmid pBR-XJ160 is an infectious full-length cDNA clone of XJ-160 virus, from which rescued virus BR-XJ160 can be obtained by transcription in vitro and transfection. The BR-XJ160 virus raised in BHK-21 cells was indistinguishable from the XJ-160 virus in its biological properties, including its plaque morphology, growth kinetics and suckling mouse neurovirulence. On basis of pBR-XJ160, the effects of substitutions within nonstructural protein 1 (nsP1) or nsP2 on the infectivity and pathogenesis of Sindbis virus (SINV) have been investigated. We have also confirmed the essential role of E2 glycoprotein, especially the domain of 145-150 (amino acid) aa, in SINV infection through the interaction with cellular heparan sulfate (HS). In addition, we have developed XJ-160 virus-based vector system, including replicon vector, defective helper (DH) plasmids and the packaging cell lines (PCLs). Here we provide an update of main development in the field concerned with XJ-160 virus

Systematic study of elliptic flow parameter in the relativistic nuclear collisions at RHIC and LHC energies

We employed the new issue of a parton and hadron cascade model PACIAE 2.1 to systematically investigate the charged particle elliptic flow parameter $v_2$ in the relativistic nuclear collisions at RHIC and LHC energies. With randomly sampling the transverse momentum $x$ and $y$ components of the particles generated in string fragmentation on the circumference of an ellipse instead of circle originally, the calculated charged particle $v_2(\eta)$ and $v_2(p_T)$ fairly reproduce the corresponding experimental data in the Au+Au/Pb+Pb collisions at $\sqrt{s_{NN}}$=0.2/2.76 TeV. In addition, the charged particle $v_2(\eta)$ and $v_2(p_T)$ in the p+p collisions at $\sqrt s$=7 TeV as well as in the p+Au/p+Pb collisions at $\sqrt{s_{NN}}$=0.2/5.02 TeV are predicted.Comment: 7 pages, 5 figure

1-[5-(2-Chloro­phen­yl)-5-hy­droxy-3-methyl-4,5-dihydro-1H-pyrazol-1-yl]­ethanone

The title compound, C12H13ClN2O2, crystallizes with two independent but very similar mol­ecules (A and B) in the asymmetric unit. The pyrazole ring in each mol­ecule has an envelope conformation. The dihedral angle between the pyrazole ring mean plane and the benzene ring is 86.07 (14)° in A and 85.99 (14)° in B. In the crystal, the A and B mol­ecules are linked via a pair of O—H⋯O hydrogen bonds, forming dimers. These dimers are further linked via C—H⋯O inter­actions to form –A–B–A–B– chains propagating along the c-axis direction