Exposure to the complement C5b-9 complex sensitizes 661W photoreceptor cells to both apoptosis and necroptosis.

The loss of photoreceptors is the defining characteristic of many retinal degenerative diseases, but the mechanisms that regulate photoreceptor cell death are not fully understood. Here we have used the 661W cone photoreceptor cell line to ask whether exposure to the terminal complement complex C5b-9 induces cell death and/or modulates the sensitivity of these cells to other cellular stressors. 661W cone photoreceptors were exposed to complete normal human serum following antibody blockade of CD59. Apoptosis induction was assessed morphologically, by flow cytometry, and on western blotting by probing for cleaved PARP and activated caspase-3. Necroptosis was assessed by flow cytometry and Sirtuin 2 inhibition using 2-cyano-3-[5-(2,5-dichlorophenyl)-2-furyl]-N-5-quinolinylacrylamide (AGK2). The sensitivity of 661W cells to ionomycin, staurosporine, peroxide and chelerythrine was also investigated, with or without prior formation of C5b-9. 661W cells underwent apoptotic cell death following exposure to C5b-9, as judged by poly(ADP-ribose) polymerase 1 cleavage and activation of caspase-3. We also observed apoptotic cell death in response to staurosporine, but 661W cells were resistant to both ionomycin and peroxide. Interestingly, C5b-9 significantly increased 661W sensitivity to staurosporine-induced apoptosis and necroptosis. These studies show that low levels of C5b-9 on 661W cells can induce apoptosis, and that C5b-9 specifically sensitizes 661W cells to certain apoptotic and necroptotic pathways. Our observations provide new insight into the potential role of the complement system in photoreceptor loss, with implications for the molecular aetiology of retinal disease

Multi-gigabit microwave and millimeter-wave communications research at CSIRO

© 2014 IEEE. High speed and long range wireless backhauls are cost-effective alternatives to fibre networks and becoming more and more attractive as the demand for broadband wireless services grows rapidly in recent years. However, current commercially available wireless backhaul systems neither provide sufficiently high speed nor meet the requirements to achieve both high speed and long range at the same time with sufficiently low latency for targeted applications. Traditional microwave systems can achieve long transmission range, but the data rates are limited to a few hundred Mega bits per second only. Multi-Gigabit wireless communications can be achieved using millimetre-wave (mm-wave) frequency bands, especially the E-bands, but the practical transmission range is still a major weakness. In this paper, the state-of-the-art microwave and mm-wave technologies developed at the Commonwealth Scientific and Industrial Research Organization (CSIRO) are introduced to demonstrate CSIRO's technology leadership in multi-Gigabit wireless communications research and development. The technology trends in multi-Gigabit wireless communications are also discussed and various recently developed microwave and mm-wave systems are compared. It is hoped that this paper will stimulate further research interest and industry development

Unified out-of-band emission reduction with linear complexity for OFDM

© 2014 IEEE. This paper proposes a unified out-of-band emission (OOBE) reduction framework with linear complexity for orthogonal frequency-division multiplexing (OFDM) systems. Unlike conventional spectral precoding approaches which use orthogonal precoding matrixes, this framework composes cancellation signals from the linear combinations of data symbols and minimizes the average OOBE power with a general least-squares solution. A joint frequency domain cancellation subcarrier and data domain cancellation symbol allocation scheme is also proposed for discrete Fourier transform precoded OFDM, by which the overall signal processing complexity of the OFDM transceiver is further reduced without impact on other system performance. The advantages of the proposed scheme is verified both analytically and by simulation as compared with some well-known low-complexity OOBE reduction schemes

Joint transmitter and receiver I/Q imbalance estimation in presence of carrier frequency offset

© 2015 IEEE. This paper proposes a simple frequency domain joint transmitter and receiver I/Q imbalance estimation method which exploits the phase rotation introduced by carrier frequency offset. Using two frequency domain training sequences inserted in each transmission frame, the transmitter and receiver I/Q imbalances can be jointly estimated over multiple frames. The transmitter I/Q imbalance parameter can be fed back to the transmitter for I/Q imbalance pre-compensation, whereas the receiver I/Q imbalance can be compensated locally followed by conventional frequency domain equalization. Numerical simulation results show that the image rejection ratios for both transmitter and receiver after I/Q imbalance compensation can be improved to over 50 dB which is necessary for multichannel systems with high order modulation and wide transmission bandwidth

Massive hybrid antenna array for millimeter-wave cellular communications

© 2002-2012 IEEE. A massive hybrid array consists of multiple analog subarrays, with each subarray having its digital processing chain. It offers the potential advantage of balancing cost and performance for massive arrays and therefore serves as an attractive solution for future millimeter-wave (mm- Wave) cellular communications. On one hand, using beamforming analog subarrays such as phased arrays, the hybrid configuration can effectively collect or distribute signal energy in sparse mm-Wave channels. On the other hand, multiple digital chains in the configuration provide multiplexing capability and more beamforming flexibility to the system. In this article, we discuss several important issues and the state-of-the-art development for mm-Wave hybrid arrays, such as channel modeling, capacity characterization, applications of various smart antenna techniques for single-user and multiuser communications, and practical hardware design. We investigate how the hybrid array architecture and special mm-Wave channel property can be exploited to design suboptimal but practical massive antenna array schemes. We also compare two main types of hybrid arrays, interleaved and localized arrays, and recommend that the localized array is a better option in terms of overall performance and hardware feasibility

Energetics of intrinsic defects in NiO and the consequences for its resistive random access memory performance

Energetics for a variety of intrinsic defects in NiO are calculated using state-of-the-art ab initio hybrid density functional theory calculations. At the O-rich limit, Ni vacancies are the lowest cost defect for all Fermi energies within the gap, in agreement with the well-known p-type behaviour of NiO. However, the ability of the metal electrode in a resistive random access memory metal-oxide-metal setup to shift the oxygen chemical potential towards the O-poor limit results in unusual NiO behaviour and O vacancies dominating at lower Fermi energy levels. Calculated band diagrams show that O vacancies in NiO are positively charged at the operating Fermi energy giving it the advantage of not requiring a scavenger metal layer to maximise drift. Ni and O interstitials are generally found to be higher in energy than the respective vacancies suggesting that significant recombination of O vacancies and interstitials does not take place as proposed in some models of switching behaviour.The authors thank the Engineering and Physical Sciences Research Council for funding from EPSRC Grant No. EP/M009297.This is the accepted manuscript. The final version is available at http://scitation.aip.org/content/aip/journal/apl/107/12/10.1063/1.4931751

Population assessment of future trajectories in coronary heart disease mortality.

Background: Coronary heart disease (CHD) mortality rates have been decreasing in Iceland since the 1980s, largely reflecting improvements in cardiovascular risk factors. The purpose of this study was to predict future CHD mortality in Iceland based on potential risk factor trends. Methods and findings: The previously validated IMPACT model was used to predict changes in CHD mortality between 2010 and 2040 among the projected population of Iceland aged 25–74. Calculations were based on combining: i) data on population numbers and projections (Statistics Iceland), ii) population risk factor levels and projections (Refine Reykjavik study), and iii) effectiveness of specific risk factor reductions (published meta-analyses). Projections for three contrasting scenarios were compared: 1) If the historical risk factor trends of past 30 years were to continue, the declining death rates of past decades would level off, reflecting population ageing. 2) If recent trends in risk factors (past 5 years) continue, this would result in a death rate increasing from 49 to 70 per 100,000. This would reflect a recent plateau in previously falling cholesterol levels and recent rapid increases in obesity and diabetes prevalence. 3) Assuming that in 2040 the entire population enjoys optimal risk factor levels observed in low risk cohorts, this would prevent almost all premature CHD deaths before 2040. Conclusions: The potential increase in CHD deaths with recent trends in risk factor levels is alarming both for Iceland and probably for comparable Western populations. However, our results show considerable room for reducing CHD mortality. Achieving the best case scenario could eradicate premature CHD deaths by 2040. Public health policy interventions based on these predictions may provide a cost effective means of reducing CHD mortality in the future

Testing and comparing two self-care-related instruments among older Chinese adults

Objectives The study aimed to test and compare the reliability and validity, including sensitivity and specificity of the two self-care-related instruments, the Self-care Ability Scale for the Elderly (SASE), and the Appraisal of Self-care Agency Scale-Revised (ASAS-R), among older adults in the Chinese context. Methods A cross-sectional design was used to conduct this study. The sample consisted of 1152 older adults. Data were collected by a questionnaire including the Chinese version of SASE (SASE-CHI), the Chinese version of ASAS-R (ASAS-R-CHI) and the Exercise of Self-Care Agency scale (ESCA). Homogeneity and stability, content, construct and concurrent validity, and sensitivity and specificity were assessed. Results The Cronbach's alpha (α) of SASE-CHI was 0.89, the item-to-total correlations ranged from r = 0.15 to r = 0.81, and the test-retest correlation coefficient (intra-class correlation coefficient, ICC) was 0.99 (95% CI, 0.99±1.00; P<0.001). The Cronbach's α of ASAS-R-CHI was 0.78, the item-to-total correlations ranged from r = 0.20 to r = 0.65, and the test-retest ICC was 0.95 (95% CI, 0.92±0.96; P<0.001). The content validity index (CVI) of SASE-CHI and ASAS-R-CHI was 0.96 and 0.97, respectively. The findings of exploratory and confirmatory factor analyses (EFA and CFA) confirmed a good construct validity of SASE-CHI and ASAS-R-CHI. The Pearson's rank correlation coefficients, as a measure of concurrent validity, between total score of SASE-CHI and ESCA and ASAS-R-CHI and ESCA were assessed to 0.65 (P<0.001) and 0.62 (P<0.001), respectively. Regarding ESCA as the criterion, the area under the receiver operator characteristic (ROC) curve for the cut-point of SASE-CHI and ASAS-R-CHI were 0.93 (95% CI, 0.91±0.94) and 0.83 (95% CI, 0.80±0.86), respectively. Conclusion There is no significant difference between the two instruments. Each has its own characteristics, but SASE-CHI is more suitable for older adults. The key point is that the users can choose the most appropriate scale according to the specific situation.publishedVersionNivå

Operator theory and function theory in Drury-Arveson space and its quotients

The Drury-Arveson space $H^2_d$, also known as symmetric Fock space or the $d$-shift space, is a Hilbert function space that has a natural $d$-tuple of operators acting on it, which gives it the structure of a Hilbert module. This survey aims to introduce the Drury-Arveson space, to give a panoramic view of the main operator theoretic and function theoretic aspects of this space, and to describe the universal role that it plays in multivariable operator theory and in Pick interpolation theory.Comment: Final version (to appear in Handbook of Operator Theory); 42 page

The design-by-adaptation approach to universal access: learning from videogame technology

This paper proposes an alternative approach to the design of universally accessible interfaces to that provided by formal design frameworks applied ab initio to the development of new software. This approach, design-byadaptation, involves the transfer of interface technology and/or design principles from one application domain to another, in situations where the recipient domain is similar to the host domain in terms of modelled systems, tasks and users. Using the example of interaction in 3D virtual environments, the paper explores how principles underlying the design of videogame interfaces may be applied to a broad family of visualization and analysis software which handles geographical data (virtual geographic environments, or VGEs). One of the motivations behind the current study is that VGE technology lags some way behind videogame technology in the modelling of 3D environments, and has a less-developed track record in providing the variety of interaction methods needed to undertake varied tasks in 3D virtual worlds by users with varied levels of experience. The current analysis extracted a set of interaction principles from videogames which were used to devise a set of 3D task interfaces that have been implemented in a prototype VGE for formal evaluation