Additional file 1: of High serum levels of pregenomic RNA reflect frequently failing reverse transcription in hepatitis B virus particles

Figures S1-S3. In order to study the size and polyadenylation of HBV RNA in serum, samples from four HBeAg-positive patients was analysed by different PCR strategies. (DOCX 1019 kb

Tenth, 25^th, 50^th, 75^th, and 90^th percentiles of HCV RNA level in relation to <i>IL28B</i>.

<p>Tenth, 25^th, 50^th, 75^th, and 90^th percentiles of HCV RNA level in relation to <i>IL28B</i>.</p

Study enrollment and disposition of patients.

<p>Study enrollment and disposition of patients.</p

Baseline characteristics of patients with a valid liver stiffness measurement.

<p>Values are expressed as n^a, median (IQR)^b or percentage^e</p>c<p>n = 318, n = 34, and n = 91 for genotype 1, 2, and 3 respectively</p>d<p>n = 152, n = 6, and n = 51 for genotype 1, 2, and 3 respectively</p

Frequency distribution of <i>IL28B</i> variants in relation to HCV genotypes 1-3 among treatment-naïve patients.

<p>Chi-squared (<i>χ</i>²)-test was used to compare differences in distribution.</p

Baseline characteristics according to HCV genotype and IL28 B genetic variations (<i>rs12979860</i>).

<p>Values are expressed as median (IQR)^a or n^b</p>c<p>n = 173 genotype 1, n = 58 genotype 3</p>d<p>n = 373 genotype 1, n = 109 genotype 3</p

Tenth, 25^th, 50^th, 75^th, and 90^th percentiles of APRI score in relation to <i>IL28B</i> variants for genotypes 1, 2 and 3.

<p>Tenth, 25^th, 50^th, 75^th, and 90^th percentiles of APRI score in relation to <i>IL28B</i> variants for genotypes 1, 2 and 3.</p

Scatter plot comparing the New index (NoLI) with liver stiffness measured by transient elastography.

<p>The Spearman correlation coefficient was 0.542 (p<0.001).</p

Baseline characteristics in the (A) exploratory set and (B) the validation set according to presence of cirrhosis in liver biopsy.

<p>All values are presented as median (interquartile range; IQR) or percentage.</p>a<p>n = 28.</p>b<p>n = 166.</p>c<p>n = 6.</p>d<p>n = 60.</p

Receiver operator characteristics curves plotting sensitivity against specificity for prediction of cirrhosis in the exploratory set (n = 278) for the new index (NoLI) in comparison with some other biochemical indices.

<p>The AUROC for NoLI was 0.91 (95% CI 0.86–0.96). The corresponding AUROC for the other indices in the exploratory set were for FIB4 0.81 (95% CI 0.75–0.87), Lok 0.79 (95% CI 0.71–0.87), APRI 0.81 (95% CI 0.74–0.88), and for GUCI 0.81 (95% CI 0.74–0.88).</p