Use of GnRH Agonist (Desloreline) in Combination with PGF(2 alpha) on the Termination of Pregnancy in Bitches

Ten bitches whose sexual cycle periods were determined with vaginal cytology to be mated at appropriate time were used in this study. Desloreline implant (Suprelorin (R)) was inserted under the dorsal skin of bitches that were determined to be pregnant using ultrasonography 20 days after the first day of mating. Ten days after the implant insertion, single dose PGF(2 alpha) (cloprostenol, 20 mu g/kg, IM) was administered. The pregnancy was terminated on all bitches that received this procedure. It was determined that the level of progesterone decreased from an average of 28.87 +/- 11.86 ng/ml on the day of implant insertion (day 20-21 of pregnancy) to 0.43 +/- 0.31 ng/ml when the abortion was completed. It was observed that after the implant insertion, the abortion was completed in an average of 3.9 +/- 0.7 days following PGF(2 alpha) administration. It has been observed that abortions was completed in an average of 2,3 +/- 0.48 days after the first vaginal discharge was seen. In conclusion, it was determined that Desloreline, a GnRH agonist, successfully could terminate pregnancy when it is combined with a single dose of PGF(2 alpha) administration in bitches

Administration of GnRH treatment prior to ovsynch protocol to stimulate ovarian cycle in cows with functional anoestrus

The study aimed to stimulate ovarian cycle and increase conception rates with administration of GnRH prior to the synchronisation of ovulation; by the use of Ovsynch protocol in cows (n=58) with anoestrus. For group I GnRH was administered one week prior to Ovsynch protocol. For group II, only Ovsynch protocol was applied. The synchronisation rate was statistically higher (P = 2.5. In conclusion, additional GnRH treatment one,week prior to Ovsynch protocol, positively affected the conception rate and ovarian cycling, and this programme Could be used for the treatment of functional anoestrus in cows

Effects of levamisole on pregnancy in ewes

The effects of levamisole, as an anthelmintic or immunomodulator, on pregnancy were investigated in ewes. Immunomodulatory and anthelmintic doses of levamisole and physiologic saline were injected on days 0 (oestrus), 7, 14, and 21 in group I (immunomodulatory dose; n=7), group II (anthelmintic dose; n=6), and group III (control; n=6), respectively. The first service pregnancy rate, tended to be lower (P<0.06) in group I (28.6%) compared to that of groups II (83.3%) and III (83.3%). Plasma progesterone (P-4) concentration was significantly higher (P<0.05) in group II compared to that of group III on day 3, and there was a treatment x day interaction (P<0.05). No effect of levamisole treatments on plasma P-4 concentrations was detected once pregnancy was established. There was a numeric decline in plasma cholesterol concentrations in group II, compared to group I or III on day 3. In conclusion, levamisole as an immunomodulator is detrimental for the establishment of pregnancy, possibly by stimulating general and intrauterine immunity

The effect of administration of testosterone antibody, beta-Carotene and vitamin E on multiple pregnancy and MDA (Malondialdehyde) in Tuj breed sheep in non-breeding season

In this study, we aimed an increase in Multiple pregnancy and a decrease in MDA (Malondialdehyde) levels in Tuj breed sheep which are in non-breeding season by administration of testosterone antibody, beta-carotene and vitamin E. Testosterone antibody was obtained from a castrated Tuj ram following injections of testosterone. Experiment was carried Out in 29 sheep divided into three groups. Group I (n=10) was administered testosterone antibody, beta-carotene and vitamin E one week prior to placement of vaginal sponges. Group II (n=9) was administered testosterone antibody alone and Group III (n=10) was control group. Oestrus rates were detected as 90%, 88.9% and 100% in Groups I,II and III, respectively. Pregnancy rates were found 40%, 33.3% and 50% in Groups I,II and III, respectively. Multiple pregnancy was seen in Groups I and II but not in control group. MDA levels were increased in all sheep regardless of pregnancy on day 28 after vaginal sponge application, but no significant difference was detected between the gropus((P > 0.05). It was concluded that administration of testosterone antibody and beta-carotene caused an increase in multiple pregnancy rate in sheep

Effect of L-Carnitine administration on energy metabolism during periparturient period in ewes

Two experiments were conducted to investigate the effect of L-Carnitine administration on energy metabolism during periparturient period in fat tailed ewes. In Experiment 1, L-Carnitine (I g/50 kg, Treatment 1, n=8) and physiologic saline (Treatment 11; n=8) were administered subcutaneously weekly until lambing for seven or eight weeks. In Experiment 11, L-Carnitine (0.5 g/50 kg, Treatment 1, n=6) and physiologic saline (Treatment 11; n=5) administered subcutaneously twice a week until lambing for at least three weeks. Blood samples were collected during treatments and one week after lambing to determine serum non esterified faty acid (NEFA), beta-hidroxybutiric acid (BHBA), total triglyceride and glucose concentrations. In experiment 1, NEFA concentrations significantly (P<0.01) increased until parturition followed by sudden decrease, and the concentrations were significantly lower (P<0.01) in L-Carnitine group. In Experiment 11, serum NEFA concentrations were significantly (P<0.01) lower at and one week prior to lambing in ewes treated with more than four weeks compared to those treated with four or less than four weeks prepartum. Serum concentrations gradually (P<0.01) increased until parturition followed by sudden decrease in all groups. However, serum NEFA concentrations did not differ in Experiment II. In conclusion, L-Carnitine administration during periparturient period decreased serum NEFA concentrations without any changes in serum BHBA, triglyceride and glucose concentrations

The effectiveness of intravaginal progesterone insert in Ovsynch and resynchronization protocols in cyclic and non-cyclic Holstein heifers

In the present study, it was aimed to compare of resynchronization with used progesterone (P4) releasing intravaginal device (PRID), with modified ovulation synchronization in cyclic and non-cyclic Holstein heifers. Animals were randomly divided into two groups; Group I (GI, n=87) and Group II (GII, n=88). Fixed-time artificial insemination (FTAI) following modified Ovsynch protocol with PRID insertion, was performed to all animals. In GI, used PRID was reinserted to the same animals, 15 days after the first FTAI (day 25) for 12 days. Pregnancies were detected by ultrasound in all groups on day 37. For resynchronization, prostaglandin (PG) F-2 alpha was applied to non-pregnant animals on day 37. In GI, following 48 hours from PGF(2 alpha) injection, the gonadotropin-releasing hormone was administrated, and after 18 hours the second FTAI was performed. No additional treatments were applied to the nonpregnant animals found in GII, and heifers were taken to the routine service program. In both groups, the second pregnancy diagnosis was performed 27 days after inseminations by transrectal ultrasonography. Pregnancy rates (PR) following the first and overall inseminations in cyclic and non-cyclic heifers (GI and GII) were detected as 66.4% and 52.3% (P>0.05); 76.3% and 59.1% (P>0.05), respectively. In conclusion, the usage of PRID in the Ovsynch and resynchronization protocol resulted in higher PRs of non-cyclic heifers than cyclic heifers. Moreover, this study reveals that the initial P4 levels of non-cyclic animals should be used as a determinant for pregnancy success in dairy heifers