Proteins and metabolites as indicators of flours quality and nutritional properties of two durum wheat varieties grown in different Italian locations

Durum wheat is an important food source in Mediterranean countries, and Italy is the major producer of durum wheat in Europe. The quality of durum wheat flours depends on the type and amount of gluten proteins and starch while flour nutritional value rests on metabolite contents such as polyphenols. In this work, two Italian cultivars, Iride and Svevo, were analyzed for two years (2016–2017) in four Italian regions to explore how the environment affects: (i) reserve proteome; (ii) starch content and composition; and (iii) free, conjugated, bound phenolics and antioxidant capacity. The impact of environmental and meteorological conditions was significant for many traits. Regardless of the cultivation site, in 2017, a year with less rainfall and a higher temperature during grain filling, there was an increase in low molecular weight glutenins, in the glutenin/gliadin ratio and in the A–type starch granules size, all parameters of higher technological quality. In the same year, the cultivars showed higher amounts of polyphenols and antioxidant capacity. In conclusion, the two wheat cultivars, selected for their medium to high yield and their good quality, had higher performances in 2017 regardless of their sowing locations

AN ACTIVE LEARNING PROCEDURE FOR THE INTERAURAL TIME DIFFERENCE DISCRIMINATION THRESHOLD

Measuring the auditory lateralization elicited by interaural time difference (ITD) cues involves the estimation of a psychometric function (PF). The shape of this function usually follows from the analysis of the subjective data and models the probability of correctly localizing the angular position of a sound source. The present study describes and evaluates a procedure for progressively fitting a PF, using Gaussian process classification of the subjective responses produced during a binary decision experiment. The process refines adaptively an approximated PF, following Bayesian inference. At each trial, it suggests the most informative auditory stimulus for function refinement according to Bayesian active learning by disagreement (BALD) mutual information. In this paper, the procedure was modified to accommodate two-alternative forced choice (2AFC) experimental methods and then was compared with a standard adaptive “three-down, one-up” staircase procedure. Our process approximates the average threshold ITD 79.4% correct level of lateralization with a mean accuracy increase of 8.9% over the Weibull function fitted on the data of the same test. The final accuracy for the Just Noticeable Difference (JND) in ITD is achieved with only 37.6% of the trials needed by a standard lateralization test

The Role of Haptics in Training and Games for Hearing-Impaired Individuals: A Systematic Review

Sensory substitution and augmentation are pivotal concepts in multi-modal perception, particularly when confronting the challenges associated with impaired or missing sense rehabilitation. The present systematic review investigates the role of haptics for the hearing impaired in training or gamified activities. We applied a set of keywords to the Scopus® and PubMed® databases, obtaining a collection of 35 manuscripts spanning 23 years. Each article has been categorized following a documented procedure and thoroughly analyzed. Our findings reveal a rising number of studies in this field in the last five years, mostly testing the effectiveness of the developed rehabilitative method (77.14%). Despite a wide variety in almost every category we analyzed, such as haptic devices, body location, and data collection, we report a constant difficulty in recruitment, reflected in the low number of hearing-impaired participants (mean of 8.31). This review found that in all six papers reporting statistically significant positive results, the vibrotactile device in use generated vibrations starting from a sound, suggesting that some perceptual aspects connected to sound are transmittable through touch. This fact provides evidence that haptics and vibrotactile devices could be viable solutions for hearing-impaired rehabilitation and training

Investigations using Csiro Hi triaxal cells for measuring the stress state of rock masses subject to mining extraction: numerical modelling of in-situ extracted core samples

The measurement of the stress state of rock, carried out in-situ using the overcoring CSIro hI Cell technique, provides valuable information about the rock mass geo-structural and stress conditions. this is particularly useful for calibrating the numerical model of natural slopes and excavations fronts and for assessing their static conditions. thus, it allows to improve workplace safety conditions in both open-pit and underground quarries. During an in-situ CSIro test, the stress release strains are measured by 12 strain gauges differently oriented in the space and the stress tensor and the material elastic parameters are then computed. the classic interpretative procedure of stress release test refers to analytical formulations that assume an extracted sample of regular cylindrical shape. however, during overcoring, it may happen that a discontinuity is intercepted, causing the extracted core to break and to assume an irregular shape. to address this challenge, in this work, a Finite Element numerical simulation of stress release was conducted basing on a 3D digital model of the irregular sample resulting from a Photogrammetric Survey. this allowed for the computation of the stress tensor for both irregularly shaped and ideal cylindrical samples. the research proceeded as it follows: i) three-dimensional modelling of the irregularly shaped core using Photogrammetric techniques and mesh Editing, which enabled the accurate representation of complex geometries; ii) numerical modelling of the irregularly shaped core containing the CSIro hI Cell through Finite Element Analysis, providing insights about stress and deformation distributions; iii) stress State of the rock calculation using a multiple Linear regression Procedure by using the coefficient matrix as determined by the core numerical modelling. the implementation of this procedure may facilitate the determination of stress state for irregularly shaped cores, and it enhances to understand how shape and rock elastic properties may influence the stress release behaviour. this comprehensive approach could allow to address challenges associated to stress assessment for irregular shaped rock cores and to improve the accuracy and applicability of geotechnical engineering methods

Differences in the distribution of stroke subtypes in a UK black stroke population - final results from the South London Ethnicity and Stroke Study.

BACKGROUND: Stroke incidence is increased in Black individuals but the reasons for this are poorly understood. Exploring the differences in aetiological stroke subtypes, and the extent to which they are explained by conventional and novel risk factors, is an important step in elucidating the underlying mechanisms for this increased stroke risk. METHODS: Between 1999 and 2010, 1200 black and 1200 white stroke patients were prospectively recruited from a contiguous geographical area in South London in the UK. The Trial of Org 10172 (TOAST) classification was used to classify stroke subtype. Age- and sex-adjusted comparisons of socio-demographics, traditional vascular risk factors and stroke subtypes were performed between black and white stroke patients and between Black Caribbean and Black African stroke patients using age-, sex-, and social deprivation-adjusted univariable and multivariable logistic regression analyses. RESULTS: Black stroke patients were younger than white stroke patients (mean (SD) 65.1 (13.7) vs. 74.8 (13.7) years). There were significant differences in the distribution of stroke subtypes. Small vessel disease stroke was increased in black patients versus white patients (27 % vs. 12 %; OR, 2.74; 95 % CI, 2.19-3.44), whereas large vessel and cardioembolic stroke was less frequent in black patients (OR, 0.59; 95 % CI, 0.45-0.78 and OR, 0.61; 95 % CI, 0.50-0.74, respectively). These associations remained after controlling for traditional vascular risk factors and socio-demographics. Black Caribbean patients appeared to have an intermediate risk factor and stroke subtype profile between that found in Black African and white stroke patients. Cardioembolic stroke was more strongly associated with Black Caribbean ethnicity versus Black African ethnicity (OR, 1.48; 95 % CI, 1.04-2.10), whereas intracranial large vessel disease was less frequent in Black Caribbean patients versus Black African subjects (OR, 0.44; 95 % CI, 0.24-0.83). CONCLUSIONS: Clear differences exist in stroke subtype distribution between black and white stroke patients, with a marked increase in small vessel stroke. These could not be explained by differences in the assessed traditional risk factors. Possible explanations for these differences might include variations in genetic susceptibility, differing rates of control of vascular risk factors, or as yet undetermined environmental risk factors.This work was supported by a Stroke Association (UK) Programme Grant (PROG 3) (www.stroke.org.uk) and the National Institute for Health Research (NIHR) Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust and King's College London (http://www.guysandstthomasbrc.nihr.ac.uk). The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health. Loes Rutten-Jacobs was supported by a British Heart Foundation Immediate Research Fellowship (FS/15/61/31626) (www.bhf.org.uk). Hugh Markus is supported by an NIHR Senior Investigator award (www.nihr.ac.uk) and his work is supported by the NIHR Cambridge University Hospital Trusts Comprehensive BRC (www.cambridge-brc.org.uk)

Preferential survival in models of complex ad hoc networks

There has been a rich interplay in recent years between (i) empirical investigations of real world dynamic networks, (ii) analytical modeling of the microscopic mechanisms that drive the emergence of such networks, and (iii) harnessing of these mechanisms to either manipulate existing networks, or engineer new networks for specific tasks. We continue in this vein, and study the deletion phenomenon in the web by following two different sets of web-sites (each comprising more than 150,000 pages) over a one-year period. Empirical data show that there is a significant deletion component in the underlying web networks, but the deletion process is not uniform. This motivates us to introduce a new mechanism of preferential survival (PS), where nodes are removed according to a degree-dependent deletion kernel. We use the mean-field rate equation approach to study a general dynamic model driven by Preferential Attachment (PA), Double PA (DPA), and a tunable PS, where c nodes (c<1) are deleted per node added to the network, and verify our predictions via large-scale simulations. One of our results shows that, unlike in the case of uniform deletion, the PS kernel when coupled with the standard PA mechanism, can lead to heavy-tailed power law networks even in the presence of extreme turnover in the network. Moreover, a weak DPA mechanism, coupled with PS, can help make the network even more heavy-tailed, especially in the limit when deletion and insertion rates are almost equal, and the overall network growth is minimal. The dynamics reported in this work can be used to design and engineer stable ad hoc networks and explain the stability of the power law exponents observed in real-world networks.Comment: 9 pages, 6 figure

Stress–Strain Investigation of the Rock Mass Based on Overcoring with CSIRO HI Cell Test and Numerical Modeling: A Case Study from an Italian Underground Marble Quarry

The present research illustrates the application of a methodological approach to studying the stress–strain distribution in a marble quarry of the Apuan Alps mining area (Italy). This study has been carried out in the framework of a project involving the University of Siena and the UOC Ingegneria Mineraria—USL Toscana Nord-Ovest, Tuscany Region. This stress–strain analysis aims foremost to monitor the slope stability conditions to guarantee a safe workplace for the personnel involved in mining activities, and to enable more sustainable long-term planning for excavation and production. The involved survey activities are as follows: (i) terrestrial laser scanning; (ii) engineering–geological data mapping; and (iii) in situ marble stress measuring through four CSIRO-type cell tests executed in different locations and at various depths within the underground excavation walls. The gathered data converged into numerical models of the quarry, both in 2D (DEM) and 3D (FEM), calibrated by in situ stress results through a rigorous back analysis assessment using least squares procedures. The created models represent a valuable tool for the identification and securing of risk areas and for future excavation planning in respect of the site efficiency and safety

The Rho GDI Rdi1 regulates Rho GTPases by distinct mechanisms

© 2008 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0).The small guanosine triphosphate (GTP)-binding proteins of the Rho family are implicated in various cell functions, including establishment and maintenance of cell polarity. Activity of Rho guanosine triphosphatases (GTPases) is not only regulated by guanine nucleotide exchange factors and GTPase-activating proteins but also by guanine nucleotide dissociation inhibitors (GDIs). These proteins have the ability to extract Rho proteins from membranes and keep them in an inactive cytosolic complex. Here, we show that Rdi1, the sole Rho GDI of the yeast Saccharomyces cerevisiae, contributes to pseudohyphal growth and mitotic exit. Rdi1 interacts only with Cdc42, Rho1, and Rho4, and it regulates these Rho GTPases by distinct mechanisms. Binding between Rdi1 and Cdc42 as well as Rho1 is modulated by the Cdc42 effector and p21-activated kinase Cla4. After membrane extraction mediated by Rdi1, Rho4 is degraded by a novel mechanism, which includes the glycogen synthase kinase 3β homologue Ygk3, vacuolar proteases, and the proteasome. Together, these results indicate that Rdi1 uses distinct modes of regulation for different Rho GTPases.Deutsche Forschungsgemeinschaf