Effects of non-steroidal antiinflammatory drugs on D-serine-induced oxidative stress in vitro

WOS: 000308936500007PubMed ID: 22486999Inflammation is deleterious for organs with reduced capacity of regeneration, such as the brain. Recently, studies have focused on investigating the therapeutic effects of nonsteroidal anti-inflammatory drugs (NSAIDs) in Alzheimer's disease, Parkinson's disease, Huntington's disease, and multiple sclerosis. Excitotoxicity is the pathological process when receptors for the excitatory neurotransmitter glutamate, such as the N-methyl-D-aspartate (NMDA), receptors are overactivated. This process may be involved in neurodegenerative diseases. D-serine is one of the coagonist of NMDA receptors, and increased levels of D-serine are associated with excitotoxicity. In our study, the potential neuroprotective effects of mefenamic acid, acetaminophen, and naproxen sodium were investigated against D-serine-induced oxidative stress in the rat brain in vitro. To show their potential neuroprotective properties, NSAIDs were incubated with D-serine and reactive oxygen species (ROS), malondialdehyde, and protein carbonyl content of the brain after different treatments were measured. Our results demostrate that NSAIDs used in the present study significantly reduced ROS production, lipid peroxidation, and protein oxidation against D-serine treatment.Turkish Scientific and Technological Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Ege University Scientific Research FoundationEge University [09/ECZ/019]G.A. acknowledges a scholarship for postgraduate students obtained from the Turkish Scientific and Technological Council (TUBITAK).; This study was supported by the Ege University Scientific Research Foundation (project no.: 09/ECZ/019)

Fecal calprotectin as a factor that supports the pathogenicity of Dientamoeba fragilis

ARMAGAN, GULIZ/0000-0001-6466-2263WOS: 000514245100013PubMed: 31730996Calprotectin is a protein that is mostly released from neutrophils, monocytes, macrophages and submucosal epithelial cells. Fecal calprotectin (f-CP) is a marker of intestinal inflammation. There are some discussions about the pathogenicity of D. fragilis in the gastrointestinal tract. in this study, we investigated whether f-CP level is a factor supporting the pathogenicity of D. fragilis. the f-CP levels were evaluated in patients with only D. fragilis positive in comparison with healthy controls. Moreover, the levels of f-CP were investigated in fecal samples of D. fragilis negative patients with gastrointestinal complaints. the fecal samples were collected from three groups. Three groups of fecal samples were examined directly microscopy, trichrome staining, cultivation, enzyme immunoassay (EIA) and real-time PCR assay. in the first group (Group 1, n = 34), patient stool samples with gastrointestinal symptoms (without other pathogens) found only with D. fragilis were included. in the second group (Group 2, n = 31), there were patients' stool samples with gastrointestinal symptoms that D. fragilis was negative (but there may be other pathogenic agents). in the control group (Group 3, n = 23), we used fecal samples collected from healthy volunteers without any infection or gastrointestinal complaints. the collected fecal samples were stored at -20 degrees C until analysis. Levels of f-CP were determined by using human calprotectin ELISA kits. Total of 88 patients were enrolled in three different groups. We obtained f-CP levels as follows: 33.40 ng/mg protein in the group 1, 15.99 ng/mg protein in the group 2 and 1.54 ng/mg protein in the group 3. Statistically significant difference in f-CP levels of the group 1 and the group 2 were obtained when compared with healthy controls (p 0.99). in conclusion, increased levels of f-CP are shown as a marker of an inflammatory disease of the lower gastrointestinal tract in infected humans. There is continues controversy about the pathogenicity of D. fragilis in symptomatic and asymptomatic patients. the findings of this study contribute to the ongoing debate about the pathogenicity of D. fragilis. in our study, the potential pathogenicity of D. fragilis is associated with increased f-CP concentrations with parasite detection in the fecal samples and therefore we assume that the parasite is not only a harmless commensal. in summary, higher levels of f-CP found in D. fragilis positive patients suggest the importance of researches that support the pathogenicity of indicated parasite.Scientific Research Projects Branch Directorate of Ege University, TurkeyEge University [13-TIP-092]This study was partly supported by the grant given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant number: 13-TIP-092)

Tidegluib provides neuroprotection in Parkinson's Disease model through Nrf2/are pathway

Conference of the Society-for-Free-Radical-Research-Europe (SFRR-E) -- JUN 08-11, 2016 -- Budapest, HUNGARYWOS: 000377617900060Soc Free Rad Res Europ

Modulation of c-fos gene expressions and glutathione levels by gamma-glutamylcysteine ethyl ester in kainic acid model of neurode-generation

European Meeting of the Society-for-Free-Radical-Research-Europe (SFRR-Europe) -- OCT 10-13, 2007 -- Vilamoura, PORTUGALWOS: 000252296300223Soc Free Rad Res Europe, Portuguese Grp Free Rad, Spanish Grp Free Ra