Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice

<p>Abstract</p> <p>Background</p> <p>Porcine rotavirus infection is a significant cause of morbidity and mortality in the swine industry necessitating the development of effective vaccines for the prevention of infection. Immune responses associated with protection are primarily mucosal in nature and induction of mucosal immunity is important for preventing porcine rotavirus infection.</p> <p>Results</p> <p><it>Lactobacillus casei </it>expressing the major protective antigen VP4 of porcine rotavirus (pPG612.1-VP4) or VP4-LTB (heat-labile toxin B subunit from <it>Echerichia coli</it>) (pPG612.1-VP4-LTB) fusion protein was used to immunize mice orally. The expression of recombinant pPG612.1-VP4 and pPG612.1-VP4-LTB was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on <it>L. casei </it>was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing <it>L. casei </it>produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with pPG612.1-VP4-LTB were higher than titters from pPG612.1-VP4-immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection.</p> <p>Conclusion</p> <p>These results demonstrated that VP4 administered in the context of an <it>L. casei </it>expression system is an effective method for stimulating mucosal immunity and that LTB served to further stimulate mucosal immunity suggesting that this strategy can be adapted for use in pigs.</p

Bio-Inspired 4D Printing of Dynamic Spider Silks

Spider silks exhibit excellent mechanical properties and have promising application prospects in engineering fields. Because natural spider silk fibers cannot be manufactured on a large scale, researchers have attempted to fabricate bio-inspired spider silks. However, the fabrication of bio-inspired spider silks with dynamically tunable mechanical properties and stimulation&ndash;response characteristics remains a challenge. Herein, the 4D printing of shape memory polyurethane is employed to produce dynamic bio-inspired spider silks. The bio-inspired spider silks have two types of energy-absorbing units that can be adjusted, one by means of 4D printing with predefined nodes, and the other through different stimulation methods to make the bio-inspired spider silks contract and undergo spiral deformation. The shape morphing behaviors of bio-inspired spider silks are programmed via pre-stress assemblies enabled by 4D printing. The energy-absorbing units of bio-inspired spider silks can be dynamically adjusted owing to stress release generated with the stimuli of temperature or humidity. Therefore, the mechanical properties of bio-inspired spider silks can be controlled to change dynamically. This can further help in developing applications of bio-inspired spider silks in engineering fields with dynamic changes of environment

Arbitrarily Shaped Video Object Coding by Wavelet

Video object coding is one of the most important functionalities proposed by MPEG4. In this paper, we propose a new wavelet method to encode the texture of an arbitrarily shaped object, both for the still and for video object. The method uses the shape adaptive wavelet transform (SA-DWT) in MPEG4 still object coding, but with a computationally more efficient lifting implementation

Traditional Classroom vs E-learning in Higher Education: Difference between Students' Behavioral Engagement

We discuss traditional classroom, e-learning, behavioral engagement and difference between behavioral engagements in two kind of instruction environment. Results from variance analyses suggest that there is no significant difference between engagements of active learning in different classroom conditions, and there exist significant differences on higher-level learning of innovative and critical thinking. Our findings highlight students' behavioral engagements in two environments have no significant advantage over each other, but e-learning facilitates higher-level learning better

Arbitrarily shaped video object coding by wavelet

Video object coding is one of the most important functionalities proposed by MPEG-4. In this paper, we propose a new wavelet method to encode the texture of an arbitrarily shaped object, both for the still and for the video object. The method uses the shape adaptive wavelet transform (SA-DWT) in MPEG-4 still object coding, but with a computationally more efficient lifting im-plementation. The transformed object coefficients are then quantized and entropy encoded with a partial bit-plane embedded coder, which greatly improves the coding efficiency. We denote the coding algorithm as the video object wavelet (VOW) coder. Experimental results show that VOW significantly outperforms MPEG-4 in still object coding, and achieves a comparable per-formance in video object coding in terms of PSNR. Moreover, the VOW decoded object looks better subjectively, with less annoying blocking artifacts than that of MPEG-4. ∗ This work was done while Dr. Guiwei Xing was an intern at Microsoft Research China

Superelasticity of Geometrically Graded NiTi Shape Memory Alloys

A stress plateau with a strain of 5–8% normally occurs during the stress-induced martensite transformation (SIM) of NiTi shape memory alloys. Within the stress plateau, the correlation between the stress and strain is lost, which limits their application in certain fields which require accurate control of inelastic deformation. In order to address this limitation, a series of step-like NiTi samples with graded cross-sectional area were designed and fabricated. Multiple stress plateaus were achieved by varying the sample width and adjusting the number of steps; for instance, two and three stress plateaus were obtained in the samples with two and three steps, respectively. Also, linear force–strain response was obtained by changing gradually the width of the samples. The functional stability of the geometrically graded samples was significantly improved by incomplete recrystallization annealing (600 °C) followed by low-temperature (250 °C) aging treatment. The incompletely recrystallized specimens contained many dislocations and grain and sub-grain boundaries, which promoted the uniform precipitation of Ni4Ti3 nanoparticles during aging treatment. The homogeneously and densely dispersed Ni4Ti3 nanoparticles were able to strengthen the matrix considerably and prevent plastic activities during stress-induced martensite transformation. As a result, the functional stability of the geometrically graded NiTi samples was much improved. After aging at 250 °C for 120 h, all the samples showed a small residual strain of <1.0% after 20 loading–unloading cycles

Instability Mechanism and Control Method of Surrounding Rock of Water-Rich Roadway Roof

Surrounding rock properties and occurrence stability of a coal seam roof are prerequisites for ensuring the safe and efficient operation of mines. In this study, the mechanisms and control of weakened water-rich roadway roof slabs were investigated regarding the engineering background of water-rich roadway roof slab destabilization in a coal mine in the western Qingyang mining area. The spatial and temporal evolution law of rock deformation and damage of such roadways during excavation were determined through field measurements. First, we tested the strength of the roof slab surrounding rock in water-rich roadways with different water contents and concluded that the primary and excavation-disturbing fissures of the coal-sedimentary rock body are the external conditions for the occurrence of water–rock interaction in water-rich coal seam roadways. Moreover, the rock mechanical damage phenomenon exhibited by clay minerals in contact with water is the key factor leading to the destabilization of the water-rich roof slab’s surrounding rock. Second, a technical approach for controlling the stability of the surrounding rock by adjusting the form of the roadway section and optimizing the support parameters was proposed, and the distribution law of the surrounding rock stress field and displacement field of each section was revealed via numerical calculation. It is considered that adjustment of the stress and displacement control of the surrounding rock of the roadway is more favorable for the straight wall circular arch section. Based on the results of the sensitive orthogonal numerical simulation test, the technical parameters and scheme of the roadway support optimization were proposed. Finally, the research results were applied in the field, and the deformations of the top and bottom slab and the two ribs of the roadway after optimizing the section and support parameters were calculated as 61% and 34% lower, respectively, than those before optimization, indicating that the proposed approach can effectively control the deformation of the water-rich roadway’s surrounding rock and achieve more economic and effective stability control of this type of roadway. The research results provide new ideas and methods for controlling the surrounding rock of water-rich soft rock roadways in the western mining areas of China, which has broad application value and prospects

Influence of Layer Thickness and Raster Angle on the Mechanical Properties of 3D-Printed PEEK and a Comparative Mechanical Study between PEEK and ABS

Fused deposition modeling (FDM) is a rapidly growing 3D printing technology. However, printing materials are restricted to acrylonitrile butadiene styrene (ABS) or poly (lactic acid) (PLA) in most Fused deposition modeling (FDM) equipment. Here, we report on a new high-performance printing material, polyether-ether-ketone (PEEK), which could surmount these shortcomings. This paper is devoted to studying the influence of layer thickness and raster angle on the mechanical properties of 3D-printed PEEK. Samples with three different layer thicknesses (200, 300 and 400 μm) and raster angles (0°, 30° and 45°) were built using a polyether-ether-ketone (PEEK) 3D printing system and their tensile, compressive and bending strengths were tested. The optimal mechanical properties of polyether-ether-ketone (PEEK) samples were found at a layer thickness of 300 μm and a raster angle of 0°. To evaluate the printing performance of polyether-ether-ketone (PEEK) samples, a comparison was made between the mechanical properties of 3D-printed polyether-ether-ketone (PEEK) and acrylonitrile butadiene styrene (ABS) parts. The results suggest that the average tensile strengths of polyether-ether-ketone (PEEK) parts were 108% higher than those for acrylonitrile butadiene styrene (ABS), and compressive strengths were 114% and bending strengths were 115%. However, the modulus of elasticity for both materials was similar. These results indicate that the mechanical properties of 3D-printed polyether-ether-ketone (PEEK) are superior to 3D-printed ABS

A Method of Soil Moisture Content Estimation at Various Soil Organic Matter Conditions Based on Soil Reflectance

Soil moisture is one of the most important components of all the soil properties affecting the global hydrologic cycle. Optical remote sensing technology is one of the main parts of soil moisture estimation. In this study, we promote a soil moisture-estimating method with applications regarding various soil organic matters. The results indicate that the soil organic matter had a significant spectral feature at wavelengths larger than 900 nm. The existence of soil organic matter would lead to darker soil, and this feature was similar to the soil moisture. Meanwhile, the effect of the soil organic matter on its reflectance overlaps with the effect of soil moisture on its reflected spectrum. This can lead to the underestimation of the soil moisture content, with an MRE of 21.87%. To reduce this effect, the absorption of the soil organic matter was considered based on the Lambert&ndash;Beer law. Then, we established an SMCg-estimating model based on the radiative transform theory while considering the effect of the soil organic matter. The results showed that the effect of the soil organic matter can be effectively reduced and the accuracy of the soil moisture estimation was increased, while MRE decreased from 21.87% to 6.53%