Adult males sampled weekly with BG Sentinel Traps.

<p>Each week, adult sampling was performed the day before the weekly release of OX3604C males. Significantly higher numbers of males were collected in treatment cages with respect to control cages (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002001#pntd.0002001.s010" target="_blank">Table S3</a>) starting from Week 2 post-release (PR), indicating that transgenic males were present in large numbers over time in release cages.</p

Progeny genotypes in treatment cages.

<p>A random sample of eggs from each treatment cage collected weekly was hatched and screened for the DsRed2 marker starting from Week 0 post-release. The number of screened larvae corresponded to 10% of the eggs produced weekly per cage or a minimum of two hundred, when available.</p

ANCOVA results for egg production dynamics in paired cages A, B, C, D, and E.

<p>ANCOVA results for egg production dynamics in paired cages A, B, C, D, and E.</p

Extinction time estimated per each treatment cage assuming the estimated fitness costs from Table 1.

1<p>Minimum extinction time.</p>2<p>Maximum extinction time.</p>3<p>Mean extinction time.</p>4<p>Standard Deviation.</p>5<p>Probability of observing extinction between weeks 18–28 PR, obtained from the outcomes of 1000 simulated experiments.</p

Egg production in treatment and control cages.

<p>Weekly egg production is shown for each control and treatment cage. Production numbers were stable in all cages by week 9 after population establishment. After OX3604C male release was initiated (vertical dashed line) in the treatment cages (week 16; week 0 PR, top time axis), egg production in the control cages continued to be stable and declined slightly in the treatment cages.</p

Summary of mating competitiveness experiments.

1<p>In experiment 6 the cage number is specified.</p>2<p>At the field site location, small cages were placed in the field laboratory.</p>3<p>Number of mixed batches with the hypothesis of 1∶1 ratio of OX3604C∶GDLS2 larvae not rejected by Chi Square Test. For statistical analysis, this number was included in the number of OX3604C matings because we assumed they came from matings with heterozygous males and not from occasional double matings.</p>(*)<p> = statistically significant results.</p

OX3604C fitness cost estimates during the field cage trial per treatment cage.

1<p>Sum of square errors associated with each estimate.</p>2<p>Standard deviation.</p