Results of the tests of molecular evolution in each sublineage of <i>RanaCyL1</i> and <i>RanaCyL2</i>.

<p>Results of the tests of molecular evolution in each sublineage of <i>RanaCyL1</i> and <i>RanaCyL2</i>.</p

Summary of phylogenetic relationships in the tribe Delphinieae (cf. [7]).

<p>The sampling ratio for each of the genera, subgenera, and subgroups from this tribe is shown (the size of each colored rectangle representing the approximate number of species in each). At the bottom right hand corner, the names of the species used for the characterization of <i>Cyc</i>-like genes are specified for each of the ten groups. Schematic shapes for the dorsal sepal and corolla are shown above the branches of the tree: the dorsal sepal can be spurred (sharp black shape) or hooded (round black shape); the corolla (in blue) can be composed of four petals (two spurred and two flat), two petals (both spurred), or a single spurred petal.</p

Phylogeny of <i>Cyc</i>-like sequences in Ranunculaceae.

<p>The phylogeny is inferred using Bayesian and maximum likelihood analysis, rooted in order to group together all the <i>RanaCyL1</i> sequences in one clade, and all the <i>RanaCyL2</i> sequences in another clade. The tree topology obtained by Bayesian inference is shown. Bayesian posterior probability ≥70 and bootstrap support from maximum likelihood analysis ≥50 are given (hyphen if inferior to those thresholds). The 381-position alignment was generated from 109 <i>RanaCyL</i> (from 48 species including 27 Delphinieae) and five other Ranunculales <i>Cyc</i>-like sequences (from three species). Portions of the tree are highlighted with colors according to the subfamilies of Ranunculaceae. The species sequenced for <i>RanaCyL</i> belong to: pink: Ranunculoideae, green: Thalictroideae, purple: Coptidoideae, and orange: Glaucidioideae. A: <i>Aconitum</i>, D: <i>Delphinium</i>, G: <i>Gymnaconitum</i>, S: <i>Staphisagria</i>. The <i>RanaCyL</i> branches tested in the molecular evolution analyses are 1a and 1b in <i>RanaCyL1</i>, and 2a and 2b in <i>RanaCyL2</i>.</p

Main types of perianth organization in the tribe Delphinieae.

<p>The four sketches are not at the same scale. (A) <i>Aconitum</i> type (applicable to groups 7, 8, and 9 from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095727#pone-0095727-g001" target="_blank">Figure 1</a>). (B) <i>Delphinium</i> type (applicable to groups 1, 5, 6, and 10 from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095727#pone-0095727-g001" target="_blank">Figure 1</a>). (C) <i>Consolida</i> type (applicable to groups 3 and 4 from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095727#pone-0095727-g001" target="_blank">Figure 1</a>). (D) <i>Aconitella</i> type (applicable to group 2 from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095727#pone-0095727-g001" target="_blank">Figure 1</a>). Sepals, dorsal petals, and lateral petals (or lateral lobes of the single petal in <i>Consolida</i> and <i>Aconitella</i>) are delineated by black, blue, and purple lines, respectively. The organs responsible for covering-protecting the nectar and the sexual organs are colored in light blue and purple, respectively. Nectar is shown in orange; it is secreted and concealed in the spur of the dorsal petal(s). Androecium and gynoecium are presented as a beige disk. The limb of the single petal has three lobes (two lateral lobes, in purple, and one upper lobe, in blue) in <i>Consolida</i>, and five (two lower lobes, in purple, and three lateral and upper lobes, in blue) in <i>Aconitella</i>. The expression level of each of the four <i>RanaCyL</i> paralogs is shown in the grids for <i>Aconitum carmichaelii</i> and <i>Consolida regalis</i>, in the following perianth compartments: petals (results were available only for <i>C. regalis</i>), dorsal (D), lateral (L), and ventral (V) sepals. Increasing expression level is shown with increasing levels of red. White: no expression detected. Grey: the paralog could not be amplified. The expression profiles are schematized based on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095727#pone-0095727-g003" target="_blank">Figure 3</a>.</p