Seasonal and spatial comparisons of microzooplankton grazing and phytoplankton growth in the Bohai Bay, China

The dilution experiment technique was used in two cruises in July-August (summer) and October-November (autumn) 2020, with a total of 14 stations. The grazing impact of microzooplankton on phytoplankton in the interior of Bohai Bay was comprehensively investigated. We compared phytoplankton growth rates (μ0) and microzooplankton grazing rates (m) spatially (distance between experimental stations and shore far vs. near) and seasonally (summer vs. autumn). Both m and μ0 values were significantly higher in summer than in autumn, and the phytoplankton growth rate μ0 was positively correlated to temperature. Offshore stations showed higher values. There is no significant spatial and seasonal differences in the ratio of microzooplankton grazing rate and phytoplankton growth rate (m/μ0) indicating that daily consumption of primary production by microzooplankton was similar in the two seasons. Therefore, our research showed a close coupling between microzooplankton grazing with phytoplankton growth in the Bohai Bay

Hypoxia in the Bohai Bay, China: distributions, causes and mechanisms

The Haihe River system is a major conduit of land-based pollution into the Bohai Sea in North China, This study presented the first-ever observation of hypoxia in the Haihe River estuary, providing valuable insights into the complex interplay between physical and biochemical factors influencing hypoxia in estuarine systems. The research found seasonal variations in DO distribution in the estuaries of the Haihe and Yongdingxin Rivers. Hypoxia emerged in summer at the bottom of the Haihe River estuary and dissipated in autumn, with the minimum DO concentration of 1.85 mg/L recorded at the bottom of the HH-2 station. Within the hypoxic zone, the average DO concentration was 4.02 mg/L, and the average of DO saturation was less than 0.4. This study also identified the primary drivers of summer hypoxia as a combination of physical and biochemical factors, with water stratification due to hydrodynamic factors limiting exchange and reoxygenation between different water layers. In addition, the decomposition of organic matter in the bottom consumed a significant amount of dissolved oxygen.The significantly higher DO concentration and saturation in autumn compared to summer suggested the possible existence of seasonal hypoxia in the Haihe estuary. This study of hypoxia in the Haihe River estuary contribute to a better understanding of the factors that influence seasonal hypoxia. The findings have important implications for the management and remediation of hypoxia in the Haihe estuary and other estuarine systems

Maternal Genetic Variants of IL4/IL13 Pathway Genes on IgE With “Western or Eastern Environments/Lifestyles”

Purpose: We investigated maternal genetic effects of four IL-4/IL-13 pathway genes as well as their interactions with the "Western or Eastern lifestyles/environments" on IgE in Karelian children. Methods: This study included 609 children and their mothers. Total IgE levels in children and mothers were measured and 10 single nucleotide polymorphisms (SNPs) in IL-4, IL-4Ra, IL-13, and STAT6 were genotyped in mothers and their children. Results: The maternal G allele of IL-13 130 (rs20541) was significantly (P=0.001) associated with decreased IgE in children in the Karelian population (Pooling Finnish and Russian children), as well as in Finnish (P=0.030) and Russian children (P=0.018). The IgE levels were significantly (P=0.001) higher in Russian children whose mothers were homozygous for the G allele of the IL-4Ra 50 (rs1805010) SNP than that in Russian children of mothers who were AG heterozygotes or AA homozygotes. After accounting for children's genotypes, we observed interactive effects on children's IgE for maternal IL-13 130 genotypes (P=0.014) and maternal IL-4Ra 50 genotypes (P=0.0003) with "Western or Eastern" lifestyles/environments. With the adjustment for multiple comparisons using a false discovery rate (FDR) of 0.05, the interactive effect of the maternal IL-4Ra50 SNP was significant. Conclusion: Maternal genetic variants in IL-4/IL-13 pathway genes, such as IL-13 130 and IL-4Ra50, influenced IgE levels in school children that were independent of the children's genetic effects. These effects differ in "Western or Eastern" environments

Influence of Sludge Initial pH on Bioleaching of Excess Sludge to Improve Dewatering Performance

pH has an important effect on the physiological activity of eosinophilic microorganisms. Therefore, this study used excess sludge produced by the mixed treatment of leachate and municipal sewage to explore the impact of different sludge initial pH on microbial biochemical reactions associated with the performance of excess sludge dehydration. Shake-flask tests were performed using inoculated microorganisms and fresh excess sludge in 500 mL Erlenmeyer flasks at a ratio of 1:4, with the addition of 2 g/L S0 and 6 g/L FeS2 as energy sources. Erlenmeyer flasks were shaken for 72 h at 180 rpm and 28 °C, in a reciprocating constant homeothermic oscillating water-bath. Results show that the specific resistance to filtration (SRF) of the bioleached excess sludge decreased from (1.45~6.68) × 1012 m/kg to (1.21~14.30) × 1011 m/kg and the sedimentation rate increased from 69.00~73.00% to 81.70~85.50%. The SRF decreased from 1.45 × 1012 m/kg to 1.21 × 1011 m/kg and the sedimentation rate increased from 69.00% to 85.00%, which both reached the highest level when the initial pH of the excess sludge was 5 and the bioleaching duration was 48 h. At this time, the rates of pH reduction and oxidative redox potential (ORP) reached the highest values (69.67% and 515 mV, respectively). Illumina HiSeq PE250 sequencing results show that the dominate microbial community members were Thiomonas (relative abundance 4.59~5.44%), which oxidize sulfur and ferrous iron, and Halothiobacillus (2.56~3.41%), which oxidizes sulfur. Thus, the acidic environment can promote microbial acidification and oxidation, which can help sludge dewatering. The presence of dominant sulfur oxidation bacteria is the essential reason for the deep dehydration of the bioleached sludge

Effects of Terrestrial Inputs on Mesozooplankton Community Structure in Bohai Bay, China

Zooplankton play a pivotal role in connecting primary producers and high trophic levels, and changes in their temporal and spatial distribution may affect the entire marine ecosystem. The spatial and seasonal taxonomic composition patterns of mesozooplankton in Bohai Bay were investigated in relation to a number of water parameters. Bohai Bay is a eutrophic semi-enclosed bay with dynamic physico-chemical conditions influenced by terrestrial inputs and seawater intrusion. The results showed that under the condition of terrigenous input, the diversity of mesozooplankton species near the eutrophic Haihe River Estuary and Jiyun River Estuary was lower than that in the central Bohai Bay, with gelatinous Oikopleura dioica as the dominant species. The mesozooplankton diversity was highest in the bay mouth affected by seawater intrusion, and the dominant oceanic species, mainly copepods Corycaeus affinis, Calanus sinicus, and Oithona similis, entered the inner bay from the bay mouth. Meanwhile, the abundance of mesozooplankton in summer was significantly higher than that in autumn. Compared with historical data, the dominant species in Bohai Bay has evolved from arrow worm Sagitta crassa to copepod Paracalanus parvus, probably due to global warming, indicating the effects of human activities on the succession of mesozooplankton community

CEPC Technical Design Report -- Accelerator

International audienceThe Circular Electron Positron Collider (CEPC) is a large scientific project initiated and hosted by China, fostered through extensive collaboration with international partners. The complex comprises four accelerators: a 30 GeV Linac, a 1.1 GeV Damping Ring, a Booster capable of achieving energies up to 180 GeV, and a Collider operating at varying energy modes (Z, W, H, and ttbar). The Linac and Damping Ring are situated on the surface, while the Booster and Collider are housed in a 100 km circumference underground tunnel, strategically accommodating future expansion with provisions for a Super Proton Proton Collider (SPPC). The CEPC primarily serves as a Higgs factory. In its baseline design with synchrotron radiation (SR) power of 30 MW per beam, it can achieve a luminosity of 5e34 /cm^2/s^1, resulting in an integrated luminosity of 13 /ab for two interaction points over a decade, producing 2.6 million Higgs bosons. Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons, facilitating precise measurements of Higgs coupling at sub-percent levels, exceeding the precision expected from the HL-LHC by an order of magnitude. This Technical Design Report (TDR) follows the Preliminary Conceptual Design Report (Pre-CDR, 2015) and the Conceptual Design Report (CDR, 2018), comprehensively detailing the machine's layout and performance, physical design and analysis, technical systems design, R&D and prototyping efforts, and associated civil engineering aspects. Additionally, it includes a cost estimate and a preliminary construction timeline, establishing a framework for forthcoming engineering design phase and site selection procedures. Construction is anticipated to begin around 2027-2028, pending government approval, with an estimated duration of 8 years. The commencement of experiments could potentially initiate in the mid-2030s