A reduced order model to assist welding parameter setup

This article demonstrates the time saving in industrial process setup using numerical reduced order modelling (ROM). The numerical simulations may supply useful information to design manufacturing processes but are often time consuming and then not suited with multi-query study such as inverse problem. ROM aims at replacing the original simulation (so-called high-fidelity (HF)) by a low rank model that will run fast according to the HF simulation time. Multi-query studies will use the ROM instead of the HF simulation to save time and deliver a solution consistent with industrial timeline. To validate the solution, the HF simulation will be used. In this paper, a problem of calibration of TIG welding parameter will illustrate this approach

Voltage-gated sodium channels: new targets in cancer therapy?

Early detection and treatment of cancers have increased survival and improved clinical outcome. The development of metastases is often associated with a poor prognostic of survival. Finding early markers of metastasis and developing new therapies against their development is a great challenge. Since a few years, there is more evidence that ionic channels are involved in the oncogenic process. Among these, voltage-gated sodium channels expressed in non-nervous or non-muscular organs are often associated with the metastatic behaviour of different cancers. The aim of this review is to describe the current knowledge on the functional expression of voltage-gated sodium channels and their biological roles in different cancers such as prostate, breast, lung (small cells and non-small cells) and leukaemia. In the conclusion, we develop conceptual approaches to understand how such channels can be involved in the metastatic process and conclude that blockers targeted toward these channels are promising new therapeutic solutions against metastatic cancers

Voltage-gated sodium channels potentiate the invasive capacities of human non-small-cell lung cancer cell lines.

Ionic channel activity is involved in fundamental cellular behaviour and participates in cancerous features such as proliferation, migration and invasion which in turn contribute to the metastatic process. In this study, we investigated the expression and role of voltage-gated sodium channels in non-small-cell lung cancer cell lines. Functional voltage-gated sodium channels expression was investigated in normal and non-small-cell lung cancer cell lines. The measurement, in patch-clamp conditions, of tetrodotoxin-inhibitable sodium currents indicated that the strongly metastatic cancerous cell lines H23, H460 and Calu-1 possess functional sodium channels while normal and weakly metastatic cell lines do not. While all the cell lines expressed mRNA for numerous sodium channel isoforms, only H23, H460 and Calu-1 cells had a 250 kDa protein corresponding to the functional channel. The other cell lines also had another protein of 230 kDa which is not addressed to the membrane and might act as a dominant negative isoform to prevent channel activation. At the membrane potential of these cells, channels are partially open. This leads to a continuous entry of sodium, disrupting sodium homeostasis and down-stream signaling pathways. Inhibition of the channels by tetrodotoxin was responsible for a 40-50% reduction of in vitro invasion. These experiments suggest that the functional expression of voltage-gated sodium channels might be an integral component of the metastatic process in non-small-cell lung cancer cells probably through its involvement in the regulation of intracellular sodium homeostasis. These channels could serve both as novel markers of the metastatic phenotype and as potential new therapeutic targets

Dietary long-chain omega-3 fatty acids of marine origin: a comparison of their protective effects on coronary heart disease and breast cancers.

The relationship between high fish consumption and low mortality following coronary heart disease (CHD) and low incidence of breast cancer was first mentioned 3 decades ago. The fishes of interest are rich in omega-3 long-chain polyunsaturated fatty acids (omega-3 LC-PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which could be the active nutrients. The current consensus about cardioprotection is that omega-3 LC-PUFAs would mainly exert antiarrhythmic effects. One of the proposed mechanisms is that circulating non-esterified LC-PUFAs partition into cardiac cells membrane phospholipids and exert a direct effect on ionic channels and/or modify intracellular calcium homeostasis. In another hypothesis, changes in the metabolism of phosphoinositides would be involved and lead to the differential activation of PKC isoforms. As compared to the mechanisms proposed for the cardioprotective effects of omega-3 LC-PUFAs, less is known about the molecular mechanisms involved in breast cancers prevention. Some proposed mechanisms such as the modulation of phosphoinositides metabolism and/or modulation of intracellular calcium homeostasis, are common to both pathologies. Other hypotheses involve the alteration of the cellular redox status induced by highly peroxidizable polyunsaturated fatty acids (FA), or the modulation of gene expression, both phenomena being tightly linked to apoptosis. In this review, we report and compare some proposed mechanisms for the involvement of omega-3 LC-PUFAs in both cardiac and breast cancer protection. Deliberately, we chose to discuss only the mechanisms, which are less described in other reviews such as ionic channels in cancer, calcium homeostasis, PKC activation or matrix metalloproteinases in both cancer and cardiac models. The leitmotiv along this review is that cardio- and cancero-protective effects use common pathways. Comparison of the cellular effects might therefore help to highlight the "protective" pathways

Model Order Reduction of Parametric Mechanical Problems Involving Contacts

In the context of manufacturing of engineering structures, unavoidable process variations can cause deviations in a manufactured structural part, resulting in “non-conformance” to the tolerance specifications. Prior to assembly, numerical simulations are often used to determine the effect of the non-conformances. Based on the analysis, parts with non-conformances are either accepted, rejected or reworked. However, the computational complexity of these numerical simulations delays the manufacturing schedule significantly. A real-time simulation tool, capable of evaluating the effect of non-conformances, is necessary to eliminate the bottleneck in the decision-making process. To accelerate the simulation of structural assemblies, we propose to apply model order reduction methods on mechanical problems involving non-linear contact interfaces. Reduction of contact problems involve a special challenge of solving a non-linear inequality constrained minimization problem, including a non-negativity condition on the contact pressure. Current approaches use mixed reduced bases for displacement and contact pressure fields, an orthogonal basis for displacement and a non-negative basis for contact pressure [1,2]. The contact pressure field is not linearly separable in nature. Hence, treating the contact problem as a low rank problem can reduce accuracy of the solution. We propose to resolve the linear inseparability using over-complete dictionaries along with sparse decomposition methods. The usage of sparse decomposition methods is inspired from the sparse behaviour observed in existing reduced approach. Sparse decomposition methods include methods like LASSO, Orthogonal Matching Projection, etc [3]. Sparse methods will be adapted to take into account inequality constraints, which are a crucial part of contact mechanics problems. REFERENCES [1] A. Benaceur, A. Ern and V. Ehrlacher, A reduced basis method for parametrized variational inequalities applied to contact mechanics. Intl. J. for Numerical Methods in Engg., 121(6), 1170–1197, 2019 [2] M. Balajewicz, D. Amsallem, & C. Farhat, Projection-based model reduction for contact problems. Intl. J. for Numerical Methods in Engineering, 106(8), 644–663, 2016 [3] J. Mairal, F. Bach, & J. Ponce, Sparse modeling for image and vision processing. Foundations and Trends in Computer Graphics and Vision, 8(2–3), 85–283, 201

Polymeric nanocapsules prevent oxidation of core-loaded molecules: evidence based on the effects of docosahexaenoic acid and neuroprostane on breast cancer cells proliferation

International audienceBackground:Nanocapsules, as a delivery system, are able to target drugs and other biologically sensitive moleculesto specific cells or organs. This system has been intensively investigated as a way to protect bioactives drugs frominactivation upon interaction with the body and to ensure the release to the target. However, the mechanism ofimproved activity of the nanoencapsulated molecules is far from being understood at the cellular and subcellularlevels. Epidemiological studies suggest that dietary polyunsaturated fatty acids (PUFA) can reduce the morbidityand mortality from breast cancer. This influence could be modulated by the oxidative status of the diet and it hasbeen suggested that the anti-proliferative properties of docosahexaenoic acid (DHA) are enhanced by pro-oxidantagents Methods:The effect of encapsulation of PUFA on breast cancer cell proliferation in different oxidative mediumwas evaluated in vitro. We compared the proliferation of the human breast cancer cell line MDA-MB-231 and ofthe non-cancer human mammary epithelial cell line MCF-10A in different experimental conditions. Results:DHA possessed anti-proliferative properties that were prevented by alpha-tocopherol (an antioxidant) andenhanced by the pro-oxidant hydrogen peroxide that confirmsthat DHA has to be oxidized to exert its anti-proliferativeproperties. We also evaluated the anti-proliferative effects of the 4(RS)-4-F4t-neuroprostane, a bioactive, non-enzymaticoxygenated metabolite of DHA known to play a major role inthe prevention of cardiovascular diseases. DHA-loadednanocapsules was less potent than non-encapsulated DHA while co-encapsulation of DHA with H2O2maintainedthe inhibition of proliferation. The nanocapsules slightly improves the anti-proliferative effect in the case of4(RS)-4-F4t-neuroprostane that is more hydrophilic than DHA. Conclusion:Overall, our findings suggest that the sensitivity of tumor cell lines to DHA involves oxidized metabolites.They also indicate that neuroprostane is a metabolite participating in the growth reducing effect of DHA, but it is not thesole. These results also suggest that NC seek to enhance the stability against degradation, enhance cellular availability,and control the release of bioactive fatty acids following their lipophilicities

Dynamique transitoire des treillis de poutres soumis à des chargements impulsionnels

This research is dedicated to the simulation of the transient response of beam trusses under impulse loads. The latter lead to the propagation of high-frequency waves in such built up structures. In the aerospace industry, that phenomenon may penalize the functioning of the structures or the equipments attached to them on account of the vibrational energy carried by the waves. It is also observed experimentally that high-frequency wave propagation evolves into a diffusive vibrational state at late times. The goal of this study is then to develop a robust model of high-frequency wave propagation within three-dimensional beam trusses in order to be able to recover, for example, this diffusion regime. On account of the small wavelengths and the high modal density, the modelling of high-frequency wave propagation is hardly feasible by classical finite elements or other methods describing the displacement fields directly. Thus, an approach dealing with the evolution of an estimator of the energy density of each propagating mode in a Timoshenko beam has been used. It provides information on the local behavior of the structures while avoiding some limitations related to the small wavelengths of high-frequency waves. After a comparison between some reduced-order beam kinematics and the Lamb model of wave propagation in a circular waveguide, the Timoshenko kinematics has been selected for the mechanical modelling of the beams. It may be shown that the energy densities of the propagating modes in a Timoshenko beam obey transport equations. Two groups of energy modes have been isolated: the longitudinal group that gathers the compressional and the bending energetic modes, and the transverse group that gathers the shear and torsional energetic modes. The reflection/transmission phenomena taking place at the junctions between beams have also been investigated. For this purpose, the power flow reflection/transmission operators have been derived from the continuity of the displacements and efforts at the junctions. Some characteristic features of a high-frequency behavior at beam junctions have been highlighted such as the decoupling between the rotational and translational motions. It is also observed that the energy densities are discontinuous at the junctions on account of the power flow reflection/transmission phenomena. Thus a discontinuous finite element method has been implemented, in order to solve the transport equations they satisfy. The numerical scheme has to be weakly dissipative and dispersive in order to exhibit the aforementioned diffusive regime arising at late times. That is the reason why spectral-like approximation functions for spatial discretization, and strong-stability preserving Runge-Kutta schemes for time integration have been used. Numerical simulations give satisfactory results because they indeed highlight the outbreak of such a diffusion state. The latter is characterized by the following: (i) the spatial spread of the energy over the truss, and (ii) the equipartition of the energy between the different modes. The last part of the thesis has been devoted to the development of a time reversal processing, that could be useful for future works on structural health monitoring of complex, multi-bay trusses.Ce travail de recherche est dédié à la simulation de la réponse transitoire des assemblages de poutres soumis à des chocs. De tels chargements entraînent la propagation d’ondes haute fréquence dans l’ensemble de la structure. L’énergie qu’elles transportent peut être dommageable pour son fonctionnement ou celui des équipements embarqués. Dans des études précédentes, il a été observé sur des structures expérimentales qu’un régime vibratoire diffusif tend à s’installer pour des temps longs. Le but de cette étude est donc de développer un modèle robuste de la réponse transitoire des assemblages de poutres soumis à des chocs permettant de simuler, entre autres, cet état diffusif. Les champs de déplacement étant très oscillants et la densité modale élevée, la simulation numérique de la réponse transitoire à des chocs peut difficilement être menée par une méthode d’éléments finis classique. Une approche utilisant un estimateur de la densité d’énergie de chaque mode de propagation a donc été mise en œuvre. Elle permet d’accéder à des informations locales sur les états vibratoires, et de contourner certaines limitations intrinsèques aux longueurs d’onde courtes. Après avoir comparé plusieurs modèles de réduction cinématique de poutre à un modèle de Lamb de propagation dans un guide d’ondes circulaire, la cinématique de Timoshenko a été retenue afin de modéliser le comportement mécanique haute fréquence des poutres. En utilisant ce modèle dans le cadre de l’approche énergétique évoquée plus haut, deux groupes de modes de propagation de la densité d’énergie vibratoire dans une poutre ont été isolés : des modes longitudinaux regroupant un mode de compression et des modes de flexion, et des modes transversaux regroupant des modes de cisaillement et un mode de torsion. Il peut être également montré que l’´evolution en temps des densités d’énergie associées obéit à des lois de transport. Pour des assemblages de poutres, les phénomènes de réflexion/transmission aux jonctions ont du être pris en compte. Les opérateurs permettant de les décrire en termes de flux d’´energie ont été obtenus grâce aux équations de continuité des déplacements et des efforts aux jonctions. Quelques caractéristiques typiques d’un régime haute fréquence ont été mises en évidence, tel que le découplage entre les modes de rotation et les modes de translation. En revanche, les champs de densité d’énergie sont quant à eux discontinus aux jonctions. Une méthode d’éléments finis discontinus a donc été développée afin de les simuler numériquement comme solutions d’´equations de transport. Si l’on souhaite atteindre le régime diffusif aux temps longs, le schéma numérique doit être peu dissipatif et peu dispersif. La discrétisation spatiale a été faite avec des fonctions d’approximation de type spectrales, et l’intégration temporelle avec des schémas de Runge-Kutta d’ordre élevé du type ”strong stability preserving”. Les simulations numériques ont donné des résultats concluants car elles permettent d’exhiber le régime de diffusion. Il a été remarqué qu’il existait en fait deux limites diffusives différentes : (i) la diffusion spatiale de l’´energie sur l’ensemble de la structure, et (ii) l’équirépartition des densités d’énergie entre les différents modes de propagation. Enfin, une technique de renversement temporel a été développée. Elle pourra être utile dans de futurs travaux sur le contrôle non destructif des assemblages complexes et de grandes tailles

Couplage excitation contraction de cellules isolees ventriculaires de cobaye: effets inotropes de la frequence de stimulation et de l'etirement a l'aide de fibres de carbone

Available from INIST (FR), Document Supply Service, under shelf-number : T 81428 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEFRFranc

Les tissus péri-implantaires (étude de l'espace biologique et intérêt du tissu kératinisé)

La muqueuse péri-implantaire est plus fragile que les tissus mous parodontaux face aux agressions exogènes. Cela souligne l'importance de la création d'une anatomie adéquate des tissus mous péri-implantaires et de facteurs prothétiques optimaux pour faciliter les précautions d'hygiène et diminuer le risque d'inflammation. L'obtention et le maintien d'un environnement tissulaire sain, tissus durs et mous, sont un préalable à la thérapeutique implantaire. Pour cela, il est important d'avoir une dimension minimale d'espace biologique péri-implantaire pour obtenir une bonne cicatrisation des tissus mous. Cette zone est de ce fait considérablement précieuse, elle est le témoin de la bonne santé des reconstructions implantaires. Il paraît aussi logique de se demander si la présence de tissu kératinisé intervient dans le pronostic favorable de la survie implantaire. En effet, bien qu'aucun consensus n'existe à ce sujet, un nombre croissant de chercheurs trouvent une amélioration des indices cliniques avec la présence d'une bande de tissu kératinisé adéquate.BORDEAUX2-BU Sci.Homme/Odontol. (330632102) / SudocSudocFranceF